Biomimetic Hydroxyapatite Crystals Growth on Phosphorylated Chitosan Films by In Vitro Mineralization Used as Dental Substitute Materials
Chitosan (CS) films exhibit great potential as a substrate for the in vitro mineralization process. In this study, to mimic the formation of nanohydroxyapatite (HAP) as natural tissue, CS films coated with a porous calcium phosphate were investigated using scanning electron microscopy (SEM), Energy...
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| Veröffentlicht in: | Polymers 2023-05, Vol.15 (11), p.2470 |
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| creator | Rahmani, Fathia Larbi Bouamrane, Omar Ben Bouabdallah, Amina Atanase, Leonard I Hellal, Abdelkader Apintiliesei, Aurelian Nichita |
| description | Chitosan (CS) films exhibit great potential as a substrate for the in vitro mineralization process. In this study, to mimic the formation of nanohydroxyapatite (HAP) as natural tissue, CS films coated with a porous calcium phosphate were investigated using scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Fourier transforms infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS). Calcium phosphate coating deposited on phosphorylated derivatives of CS was obtained by a process based on phosphorylation, Ca(OH)
treatment and artificial saliva solution (ASS) immersion. The phosphorylated CS films (PCS) were obtained by partial hydrolysis of the PO
functionalities. It was demonstrated that this precursor phase could induce the growth and the nucleation of the porous calcium phosphate coating when immersed in ASS. Moreover, oriented crystals and qualitative control of calcium phosphate phases on CS matrices are obtained in a biomimetic mode. Furthermore, in vitro antimicrobial activity of PCS was evaluated against three species of oral bacteria and fungi. It revealed an increase in antimicrobial activity with minimum inhibition concentration (MIC) values of 0.10% (
), 0.05% (
and 0.025% (
) which proves their possible use as dental substitute materials. |
| doi_str_mv | 10.3390/polym15112470 |
| format | Article |
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treatment and artificial saliva solution (ASS) immersion. The phosphorylated CS films (PCS) were obtained by partial hydrolysis of the PO
functionalities. It was demonstrated that this precursor phase could induce the growth and the nucleation of the porous calcium phosphate coating when immersed in ASS. Moreover, oriented crystals and qualitative control of calcium phosphate phases on CS matrices are obtained in a biomimetic mode. Furthermore, in vitro antimicrobial activity of PCS was evaluated against three species of oral bacteria and fungi. It revealed an increase in antimicrobial activity with minimum inhibition concentration (MIC) values of 0.10% (
), 0.05% (
and 0.025% (
) which proves their possible use as dental substitute materials.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym15112470</identifier><identifier>PMID: 37299269</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antiinfectives and antibacterials ; Biomedical materials ; Biomimetics ; Biopolymers ; Calcium phosphates ; Chitosan ; Composite materials ; Crystal growth ; Dental materials ; Dentistry ; E coli ; Electron microscopes ; Fourier transforms ; Hydroxyapatite ; Infrared spectroscopy ; Mineralization ; Nanocrystals ; Nucleation ; Orthopedics ; Phosphate coatings ; Phosphorylation ; Photoelectrons ; Porous materials ; Scanning electron microscopy ; Slaked lime ; Substitutes ; Substrates ; Tissue engineering ; Transplants & implants ; X ray photoelectron spectroscopy</subject><ispartof>Polymers, 2023-05, Vol.15 (11), p.2470</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-f38f47deefcad07765d560002fac4f64653628859855617e7c5a0310f3130d6a3</citedby><cites>FETCH-LOGICAL-c416t-f38f47deefcad07765d560002fac4f64653628859855617e7c5a0310f3130d6a3</cites><orcidid>0009-0008-0863-4063 ; 0000-0002-3657-1492 ; 0009-0009-4298-1111</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255572/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255572/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37299269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahmani, Fathia</creatorcontrib><creatorcontrib>Larbi Bouamrane, Omar</creatorcontrib><creatorcontrib>Ben Bouabdallah, Amina</creatorcontrib><creatorcontrib>Atanase, Leonard I</creatorcontrib><creatorcontrib>Hellal, Abdelkader</creatorcontrib><creatorcontrib>Apintiliesei, Aurelian Nichita</creatorcontrib><title>Biomimetic Hydroxyapatite Crystals Growth on Phosphorylated Chitosan Films by In Vitro Mineralization Used as Dental Substitute Materials</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>Chitosan (CS) films exhibit great potential as a substrate for the in vitro mineralization process. In this study, to mimic the formation of nanohydroxyapatite (HAP) as natural tissue, CS films coated with a porous calcium phosphate were investigated using scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Fourier transforms infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS). Calcium phosphate coating deposited on phosphorylated derivatives of CS was obtained by a process based on phosphorylation, Ca(OH)
treatment and artificial saliva solution (ASS) immersion. The phosphorylated CS films (PCS) were obtained by partial hydrolysis of the PO
functionalities. It was demonstrated that this precursor phase could induce the growth and the nucleation of the porous calcium phosphate coating when immersed in ASS. Moreover, oriented crystals and qualitative control of calcium phosphate phases on CS matrices are obtained in a biomimetic mode. Furthermore, in vitro antimicrobial activity of PCS was evaluated against three species of oral bacteria and fungi. It revealed an increase in antimicrobial activity with minimum inhibition concentration (MIC) values of 0.10% (
), 0.05% (
and 0.025% (
) which proves their possible use as dental substitute materials.</description><subject>Antiinfectives and antibacterials</subject><subject>Biomedical materials</subject><subject>Biomimetics</subject><subject>Biopolymers</subject><subject>Calcium phosphates</subject><subject>Chitosan</subject><subject>Composite materials</subject><subject>Crystal growth</subject><subject>Dental materials</subject><subject>Dentistry</subject><subject>E coli</subject><subject>Electron microscopes</subject><subject>Fourier transforms</subject><subject>Hydroxyapatite</subject><subject>Infrared spectroscopy</subject><subject>Mineralization</subject><subject>Nanocrystals</subject><subject>Nucleation</subject><subject>Orthopedics</subject><subject>Phosphate coatings</subject><subject>Phosphorylation</subject><subject>Photoelectrons</subject><subject>Porous materials</subject><subject>Scanning electron microscopy</subject><subject>Slaked lime</subject><subject>Substitutes</subject><subject>Substrates</subject><subject>Tissue engineering</subject><subject>Transplants & implants</subject><subject>X ray photoelectron spectroscopy</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkU1PFTEUhhsiEYIs3ZImbtwM9rszK6OXzwSiieK26Z3pOCUz07HtAMM_8F979AIBu2mTPn3OeXsQekvJIecV-TCFfhmopJQJTbbQLiOaF4Ir8urZeQftp3RNYAmpFNWv0Q7XrKqYqnbR788-DH5w2df4bGliuFvsZLPPDq_ikrLtEz6N4TZ3OIz4axfS1IW49Da7Bq86n0OyIz7x_ZDwesHnI_7hcwz40o8u2t7fgwseXiXAbcJHbgQl_javE9SYocolmKKHMm_Qdgub23_Y99DVyfH31Vlx8eX0fPXpoqgFVbloedkK3TjX1rYhWivZSAXZWGtr0SqhJFesLGVVSglhna6lJZySllNOGmX5Hvq48U7zenBNDR1Bo2aKfrBxMcF68_Jm9J35GW4MJUxKqRkY3j8YYvg1u5TN4FPt-t6OLszJsJIJVcEfC0Df_YdehzmOkO8fRSjfCIsNVceQUnTtUzeUmL-DNi8GDfzB8whP9ONY-R8GJ6b6</recordid><startdate>20230526</startdate><enddate>20230526</enddate><creator>Rahmani, Fathia</creator><creator>Larbi Bouamrane, Omar</creator><creator>Ben Bouabdallah, Amina</creator><creator>Atanase, Leonard I</creator><creator>Hellal, Abdelkader</creator><creator>Apintiliesei, Aurelian Nichita</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0009-0008-0863-4063</orcidid><orcidid>https://orcid.org/0000-0002-3657-1492</orcidid><orcidid>https://orcid.org/0009-0009-4298-1111</orcidid></search><sort><creationdate>20230526</creationdate><title>Biomimetic Hydroxyapatite Crystals Growth on Phosphorylated Chitosan Films by In Vitro Mineralization Used as Dental Substitute Materials</title><author>Rahmani, Fathia ; Larbi Bouamrane, Omar ; Ben Bouabdallah, Amina ; Atanase, Leonard I ; Hellal, Abdelkader ; Apintiliesei, Aurelian Nichita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-f38f47deefcad07765d560002fac4f64653628859855617e7c5a0310f3130d6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Biomedical materials</topic><topic>Biomimetics</topic><topic>Biopolymers</topic><topic>Calcium phosphates</topic><topic>Chitosan</topic><topic>Composite materials</topic><topic>Crystal growth</topic><topic>Dental materials</topic><topic>Dentistry</topic><topic>E coli</topic><topic>Electron microscopes</topic><topic>Fourier transforms</topic><topic>Hydroxyapatite</topic><topic>Infrared spectroscopy</topic><topic>Mineralization</topic><topic>Nanocrystals</topic><topic>Nucleation</topic><topic>Orthopedics</topic><topic>Phosphate coatings</topic><topic>Phosphorylation</topic><topic>Photoelectrons</topic><topic>Porous materials</topic><topic>Scanning electron microscopy</topic><topic>Slaked lime</topic><topic>Substitutes</topic><topic>Substrates</topic><topic>Tissue engineering</topic><topic>Transplants & implants</topic><topic>X ray photoelectron spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahmani, Fathia</creatorcontrib><creatorcontrib>Larbi Bouamrane, Omar</creatorcontrib><creatorcontrib>Ben Bouabdallah, Amina</creatorcontrib><creatorcontrib>Atanase, Leonard I</creatorcontrib><creatorcontrib>Hellal, Abdelkader</creatorcontrib><creatorcontrib>Apintiliesei, Aurelian Nichita</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahmani, Fathia</au><au>Larbi Bouamrane, Omar</au><au>Ben Bouabdallah, Amina</au><au>Atanase, Leonard I</au><au>Hellal, Abdelkader</au><au>Apintiliesei, Aurelian Nichita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomimetic Hydroxyapatite Crystals Growth on Phosphorylated Chitosan Films by In Vitro Mineralization Used as Dental Substitute Materials</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2023-05-26</date><risdate>2023</risdate><volume>15</volume><issue>11</issue><spage>2470</spage><pages>2470-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>Chitosan (CS) films exhibit great potential as a substrate for the in vitro mineralization process. In this study, to mimic the formation of nanohydroxyapatite (HAP) as natural tissue, CS films coated with a porous calcium phosphate were investigated using scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Fourier transforms infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS). Calcium phosphate coating deposited on phosphorylated derivatives of CS was obtained by a process based on phosphorylation, Ca(OH)
treatment and artificial saliva solution (ASS) immersion. The phosphorylated CS films (PCS) were obtained by partial hydrolysis of the PO
functionalities. It was demonstrated that this precursor phase could induce the growth and the nucleation of the porous calcium phosphate coating when immersed in ASS. Moreover, oriented crystals and qualitative control of calcium phosphate phases on CS matrices are obtained in a biomimetic mode. Furthermore, in vitro antimicrobial activity of PCS was evaluated against three species of oral bacteria and fungi. It revealed an increase in antimicrobial activity with minimum inhibition concentration (MIC) values of 0.10% (
), 0.05% (
and 0.025% (
) which proves their possible use as dental substitute materials.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37299269</pmid><doi>10.3390/polym15112470</doi><orcidid>https://orcid.org/0009-0008-0863-4063</orcidid><orcidid>https://orcid.org/0000-0002-3657-1492</orcidid><orcidid>https://orcid.org/0009-0009-4298-1111</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Antiinfectives and antibacterials Biomedical materials Biomimetics Biopolymers Calcium phosphates Chitosan Composite materials Crystal growth Dental materials Dentistry E coli Electron microscopes Fourier transforms Hydroxyapatite Infrared spectroscopy Mineralization Nanocrystals Nucleation Orthopedics Phosphate coatings Phosphorylation Photoelectrons Porous materials Scanning electron microscopy Slaked lime Substitutes Substrates Tissue engineering Transplants & implants X ray photoelectron spectroscopy |
| title | Biomimetic Hydroxyapatite Crystals Growth on Phosphorylated Chitosan Films by In Vitro Mineralization Used as Dental Substitute Materials |
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