Nano-DMS-MaP allows isoform-specific RNA structure determination

Genome-wide measurements of RNA structure can be obtained using reagents that react with unpaired bases, leading to adducts that can be identified by mutational profiling on next-generation sequencing machines. One drawback of these experiments is that short sequencing reads can rarely be mapped to...

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Veröffentlicht in:	Nature methods 2023-06, Vol.20 (6), p.849-859
Hauptverfasser:	Bohn, Patrick, Gribling-Burrer, Anne-Sophie, Ambi, Uddhav B., Smyth, Redmond P.
Format:	Artikel
Sprache:	eng
Schlagworte:	5' Untranslated Regions 631/1647/514 631/337/2019 631/45/500 Accuracy Adducts Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Blurring Experiments Gene sequencing Genes Genomes HIV Human immunodeficiency virus Humans Immune system Isoforms Life Sciences Mutation Nanopores Next-generation sequencing Packaging Protein Isoforms - genetics Proteomics Reagents Ribonucleic acid RNA RNA - genetics RNA, Viral - chemistry RNA, Viral - genetics Sequence Analysis, RNA Signal to noise ratio
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