The GmXTH1 gene improves drought stress resistance of soybean seedlings
In order to study the role of GmXTH1 gene in alleviating drought stress, soybean seeds with GmXTH1 gene were transferred by T 4 treated with PEG6000 concentration of 0%, 5%, 10%, and 15% respectively. The germination potential, germination rate, germination index, and other indicators were measured....
Gespeichert in:
| Veröffentlicht in: | Molecular breeding 2022-01, Vol.42 (1), p.3-3, Article 3 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3 |
|---|---|
| container_issue | 1 |
| container_start_page | 3 |
| container_title | Molecular breeding |
| container_volume | 42 |
| creator | Zhang, Ye Zhang, Han-zhu Fu, Jia-yu Du, Ye-yao Qu, Jing Song, Yang Wang, Pi-wu |
| description | In order to study the role of
GmXTH1
gene in alleviating drought stress, soybean seeds with
GmXTH1
gene were transferred by T
4
treated with PEG6000 concentration of 0%, 5%, 10%, and 15% respectively. The germination potential, germination rate, germination index, and other indicators were measured. The results showed that the germination potential, germination rate, and germination index of OEA1 and OEA2 strains overexpressed in T
4
generation were significantly higher than those of the control material M18. After 0-day, 7-day, and 15-day drought stress, the analysis of seedling phenotypes and root-shoot of different T
4
generation transgenic soybean lines showed that under stress conditions, the growth of
GmXTH1
overexpression material was generally better than that of the control material M18. The growth of
GmXTH1
interference expression material was generally worse than that of the control material M18, with significant differences in plant phenotypes. The root system of
GmXTH1
overexpressed material was significantly developed compared with that of the control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of
GmXTH1
transgenic soybean material were significantly higher than those of the control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of
GmXTH1
gene in transgenic soybean was significantly increased after drought stress. The results showed that the overexpression of
GmXTH1
could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number, and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way, reducing cell membrane damage to improve drought resistance of soybean. |
| doi_str_mv | 10.1007/s11032-021-01258-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10248595</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2825502830</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-20c80d39db0ba57acd049e780c776fd5a6f188a861fb629d85d5c1c34eab8963</originalsourceid><addsrcrecordid>eNp9kU1v3CAQhlHVqkmT_IEeKku99OJmAGPgVFVRs6kUKZc99IYwjL2ObNiCHSn_PqSbpB-HXgBpnnmZ0UPIewqfKYA8z5QCZzUwWgNlQtXiFTmmQrJaS6VelzdXUHPZ8CPyLudbKE26bd-SIy456EbxY7LZ7rDazD-2V7QaMGA1zvsU7zBXPsV12C1VXhLmXJVjzIsNDqvYVzned2hDlRH9NIYhn5I3vZ0ynj3dJ2R7-W17cVVf32y-X3y9rl0jxVIzcAo8176DzgppnYdGo1TgpGx7L2zbU6WsamnftUx7Jbxw1PEGbad0y0_Il0Psfu1m9A7Dkuxk9mmcbbo30Y7m70oYd2aId4YCa5TQoiR8ekpI8eeKeTHzmB1Okw0Y12yYYkIAUxwK-vEf9DauKZT1DGtp02gOQAvFDpRLMeeE_cs0FMyjJ3PwZIon88uTeZziw597vLQ8iykAPwC5lMKA6fff_4l9ALg9nho</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2614493001</pqid></control><display><type>article</type><title>The GmXTH1 gene improves drought stress resistance of soybean seedlings</title><source>NCBI_PubMed Central（免费）</source><source>SpringerLink_现刊</source><creator>Zhang, Ye ; Zhang, Han-zhu ; Fu, Jia-yu ; Du, Ye-yao ; Qu, Jing ; Song, Yang ; Wang, Pi-wu</creator><creatorcontrib>Zhang, Ye ; Zhang, Han-zhu ; Fu, Jia-yu ; Du, Ye-yao ; Qu, Jing ; Song, Yang ; Wang, Pi-wu</creatorcontrib><description>In order to study the role of
GmXTH1
gene in alleviating drought stress, soybean seeds with
GmXTH1
gene were transferred by T
4
treated with PEG6000 concentration of 0%, 5%, 10%, and 15% respectively. The germination potential, germination rate, germination index, and other indicators were measured. The results showed that the germination potential, germination rate, and germination index of OEA1 and OEA2 strains overexpressed in T
4
generation were significantly higher than those of the control material M18. After 0-day, 7-day, and 15-day drought stress, the analysis of seedling phenotypes and root-shoot of different T
4
generation transgenic soybean lines showed that under stress conditions, the growth of
GmXTH1
overexpression material was generally better than that of the control material M18. The growth of
GmXTH1
interference expression material was generally worse than that of the control material M18, with significant differences in plant phenotypes. The root system of
GmXTH1
overexpressed material was significantly developed compared with that of the control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of
GmXTH1
transgenic soybean material were significantly higher than those of the control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of
GmXTH1
gene in transgenic soybean was significantly increased after drought stress. The results showed that the overexpression of
GmXTH1
could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number, and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way, reducing cell membrane damage to improve drought resistance of soybean.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-021-01258-5</identifier><identifier>PMID: 37309483</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Accumulation ; Antioxidants ; Area ; Biomedical and Life Sciences ; Biotechnology ; Cell membranes ; Diameters ; Drought ; Drought resistance ; Enzymes ; Fluorescence ; Gene expression ; Germination ; Life Sciences ; Malondialdehyde ; Moisture content ; Molecular biology ; Peroxidase ; Phenotypes ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Seedlings ; Seeds ; Soybeans ; Superoxide dismutase ; Transpiration ; Water content ; Water intake ; Water intakes</subject><ispartof>Molecular breeding, 2022-01, Vol.42 (1), p.3-3, Article 3</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-20c80d39db0ba57acd049e780c776fd5a6f188a861fb629d85d5c1c34eab8963</citedby><cites>FETCH-LOGICAL-c475t-20c80d39db0ba57acd049e780c776fd5a6f188a861fb629d85d5c1c34eab8963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248595/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10248595/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37309483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Ye</creatorcontrib><creatorcontrib>Zhang, Han-zhu</creatorcontrib><creatorcontrib>Fu, Jia-yu</creatorcontrib><creatorcontrib>Du, Ye-yao</creatorcontrib><creatorcontrib>Qu, Jing</creatorcontrib><creatorcontrib>Song, Yang</creatorcontrib><creatorcontrib>Wang, Pi-wu</creatorcontrib><title>The GmXTH1 gene improves drought stress resistance of soybean seedlings</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><addtitle>Mol Breed</addtitle><description>In order to study the role of
GmXTH1
gene in alleviating drought stress, soybean seeds with
GmXTH1
gene were transferred by T
4
treated with PEG6000 concentration of 0%, 5%, 10%, and 15% respectively. The germination potential, germination rate, germination index, and other indicators were measured. The results showed that the germination potential, germination rate, and germination index of OEA1 and OEA2 strains overexpressed in T
4
generation were significantly higher than those of the control material M18. After 0-day, 7-day, and 15-day drought stress, the analysis of seedling phenotypes and root-shoot of different T
4
generation transgenic soybean lines showed that under stress conditions, the growth of
GmXTH1
overexpression material was generally better than that of the control material M18. The growth of
GmXTH1
interference expression material was generally worse than that of the control material M18, with significant differences in plant phenotypes. The root system of
GmXTH1
overexpressed material was significantly developed compared with that of the control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of
GmXTH1
transgenic soybean material were significantly higher than those of the control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of
GmXTH1
gene in transgenic soybean was significantly increased after drought stress. The results showed that the overexpression of
GmXTH1
could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number, and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way, reducing cell membrane damage to improve drought resistance of soybean.</description><subject>Accumulation</subject><subject>Antioxidants</subject><subject>Area</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell membranes</subject><subject>Diameters</subject><subject>Drought</subject><subject>Drought resistance</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Germination</subject><subject>Life Sciences</subject><subject>Malondialdehyde</subject><subject>Moisture content</subject><subject>Molecular biology</subject><subject>Peroxidase</subject><subject>Phenotypes</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Soybeans</subject><subject>Superoxide dismutase</subject><subject>Transpiration</subject><subject>Water content</subject><subject>Water intake</subject><subject>Water intakes</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU1v3CAQhlHVqkmT_IEeKku99OJmAGPgVFVRs6kUKZc99IYwjL2ObNiCHSn_PqSbpB-HXgBpnnmZ0UPIewqfKYA8z5QCZzUwWgNlQtXiFTmmQrJaS6VelzdXUHPZ8CPyLudbKE26bd-SIy456EbxY7LZ7rDazD-2V7QaMGA1zvsU7zBXPsV12C1VXhLmXJVjzIsNDqvYVzned2hDlRH9NIYhn5I3vZ0ynj3dJ2R7-W17cVVf32y-X3y9rl0jxVIzcAo8176DzgppnYdGo1TgpGx7L2zbU6WsamnftUx7Jbxw1PEGbad0y0_Il0Psfu1m9A7Dkuxk9mmcbbo30Y7m70oYd2aId4YCa5TQoiR8ekpI8eeKeTHzmB1Okw0Y12yYYkIAUxwK-vEf9DauKZT1DGtp02gOQAvFDpRLMeeE_cs0FMyjJ3PwZIon88uTeZziw597vLQ8iykAPwC5lMKA6fff_4l9ALg9nho</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Zhang, Ye</creator><creator>Zhang, Han-zhu</creator><creator>Fu, Jia-yu</creator><creator>Du, Ye-yao</creator><creator>Qu, Jing</creator><creator>Song, Yang</creator><creator>Wang, Pi-wu</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220101</creationdate><title>The GmXTH1 gene improves drought stress resistance of soybean seedlings</title><author>Zhang, Ye ; Zhang, Han-zhu ; Fu, Jia-yu ; Du, Ye-yao ; Qu, Jing ; Song, Yang ; Wang, Pi-wu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-20c80d39db0ba57acd049e780c776fd5a6f188a861fb629d85d5c1c34eab8963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Accumulation</topic><topic>Antioxidants</topic><topic>Area</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell membranes</topic><topic>Diameters</topic><topic>Drought</topic><topic>Drought resistance</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Germination</topic><topic>Life Sciences</topic><topic>Malondialdehyde</topic><topic>Moisture content</topic><topic>Molecular biology</topic><topic>Peroxidase</topic><topic>Phenotypes</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Soybeans</topic><topic>Superoxide dismutase</topic><topic>Transpiration</topic><topic>Water content</topic><topic>Water intake</topic><topic>Water intakes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Ye</creatorcontrib><creatorcontrib>Zhang, Han-zhu</creatorcontrib><creatorcontrib>Fu, Jia-yu</creatorcontrib><creatorcontrib>Du, Ye-yao</creatorcontrib><creatorcontrib>Qu, Jing</creatorcontrib><creatorcontrib>Song, Yang</creatorcontrib><creatorcontrib>Wang, Pi-wu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Ye</au><au>Zhang, Han-zhu</au><au>Fu, Jia-yu</au><au>Du, Ye-yao</au><au>Qu, Jing</au><au>Song, Yang</au><au>Wang, Pi-wu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The GmXTH1 gene improves drought stress resistance of soybean seedlings</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><addtitle>Mol Breed</addtitle><date>2022-01-01</date><risdate>2022</risdate><volume>42</volume><issue>1</issue><spage>3</spage><epage>3</epage><pages>3-3</pages><artnum>3</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>In order to study the role of
GmXTH1
gene in alleviating drought stress, soybean seeds with
GmXTH1
gene were transferred by T
4
treated with PEG6000 concentration of 0%, 5%, 10%, and 15% respectively. The germination potential, germination rate, germination index, and other indicators were measured. The results showed that the germination potential, germination rate, and germination index of OEA1 and OEA2 strains overexpressed in T
4
generation were significantly higher than those of the control material M18. After 0-day, 7-day, and 15-day drought stress, the analysis of seedling phenotypes and root-shoot of different T
4
generation transgenic soybean lines showed that under stress conditions, the growth of
GmXTH1
overexpression material was generally better than that of the control material M18. The growth of
GmXTH1
interference expression material was generally worse than that of the control material M18, with significant differences in plant phenotypes. The root system of
GmXTH1
overexpressed material was significantly developed compared with that of the control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of
GmXTH1
transgenic soybean material were significantly higher than those of the control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of
GmXTH1
gene in transgenic soybean was significantly increased after drought stress. The results showed that the overexpression of
GmXTH1
could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number, and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way, reducing cell membrane damage to improve drought resistance of soybean.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37309483</pmid><doi>10.1007/s11032-021-01258-5</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1380-3743 |
| ispartof | Molecular breeding, 2022-01, Vol.42 (1), p.3-3, Article 3 |
| issn | 1380-3743 1572-9788 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10248595 |
| source | NCBI_PubMed Central（免费）; SpringerLink_现刊 |
| subjects | Accumulation Antioxidants Area Biomedical and Life Sciences Biotechnology Cell membranes Diameters Drought Drought resistance Enzymes Fluorescence Gene expression Germination Life Sciences Malondialdehyde Moisture content Molecular biology Peroxidase Phenotypes Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Seedlings Seeds Soybeans Superoxide dismutase Transpiration Water content Water intake Water intakes |
| title | The GmXTH1 gene improves drought stress resistance of soybean seedlings |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T15%3A10%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20GmXTH1%20gene%20improves%20drought%20stress%20resistance%20of%20soybean%20seedlings&rft.jtitle=Molecular%20breeding&rft.au=Zhang,%20Ye&rft.date=2022-01-01&rft.volume=42&rft.issue=1&rft.spage=3&rft.epage=3&rft.pages=3-3&rft.artnum=3&rft.issn=1380-3743&rft.eissn=1572-9788&rft_id=info:doi/10.1007/s11032-021-01258-5&rft_dat=%3Cproquest_pubme%3E2825502830%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2614493001&rft_id=info:pmid/37309483&rfr_iscdi=true |