Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line
Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance...
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| Veröffentlicht in: | Molecular breeding 2021-12, Vol.41 (12), p.75-75, Article 75 |
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| creator | Wu, Yunyu Xiao, Ning Li, Yuhong Gao, Qiang Ning, Yuese Yu, Ling Cai, Yue Pan, Cunhong Zhang, Xiaoxiang Huang, Niansheng Zhou, Changhai Ji, Hongjuan Liu, Jianju Shi, Wei Chen, Zichun Liang, Chengzhi Li, Aihong |
| description | Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing
Pigm
into C134S and showed significant differences in the panicle blast resistance. One F
2
population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL
qPBR10-1
on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore,
qPBR10-1
was verified using the BC
1
F
2
and BC
1
F
3
population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that
ORF1
and
ORF2
encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the
qPBR10-1
locus. |
| doi_str_mv | 10.1007/s11032-021-01268-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10236096</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2825503430</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-cdf160e54249ca98e8d2e83a89a28075679e6c1d1baf1d73f402eab20cf016773</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxSMEoqXwBTggS1w4YDq2E9s5oVJBqVTECsrZ8jqTxZVjb-2kEhc-O162lD8HTmNpfu-Nn17TPGXwigGo48IYCE6BMwqMS03FveaQdYrTXml9v76FBipUKw6aR6VcQRX1Uj5sDoQS0HesPWy-nw8YZz96Z2efIrFxIKOPSCa73fq4IWkk16s3nxhQ9pJYEtMNBhKSWwpxKc45hbDDtjZ6F5Csgy0zyVh8mW10SHwkK7-Z6A62Pu7Y1fHl2YfPpOrwcfNgtKHgk9t51Hx59_by9D29-Hh2fnpyQV2rupm6YWQSsGt52zvba9QDRy2s7i3XoDqpepSODWxtRzYoMbbA0a45uBGYVEocNa_3vttlPeHgauZsg9lmP9n8zSTrzd-b6L-aTboxDLiQ0Mvq8OLWIafrBctsJl8chmAjpqUYrnnXgWgFVPT5P-hVWnKs-QyX0MpOtZpViu8pl1MpGce73zAwu37Nvl9T-zU_-zWiip79meNO8qvQCog9UOoqbjD_vv0f2x9EIbDT</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2604657481</pqid></control><display><type>article</type><title>Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line</title><source>PubMed (Medline)</source><source>Springer Nature</source><creator>Wu, Yunyu ; Xiao, Ning ; Li, Yuhong ; Gao, Qiang ; Ning, Yuese ; Yu, Ling ; Cai, Yue ; Pan, Cunhong ; Zhang, Xiaoxiang ; Huang, Niansheng ; Zhou, Changhai ; Ji, Hongjuan ; Liu, Jianju ; Shi, Wei ; Chen, Zichun ; Liang, Chengzhi ; Li, Aihong</creator><creatorcontrib>Wu, Yunyu ; Xiao, Ning ; Li, Yuhong ; Gao, Qiang ; Ning, Yuese ; Yu, Ling ; Cai, Yue ; Pan, Cunhong ; Zhang, Xiaoxiang ; Huang, Niansheng ; Zhou, Changhai ; Ji, Hongjuan ; Liu, Jianju ; Shi, Wei ; Chen, Zichun ; Liang, Chengzhi ; Li, Aihong</creatorcontrib><description>Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing
Pigm
into C134S and showed significant differences in the panicle blast resistance. One F
2
population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL
qPBR10-1
on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore,
qPBR10-1
was verified using the BC
1
F
2
and BC
1
F
3
population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that
ORF1
and
ORF2
encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the
qPBR10-1
locus.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-021-01268-3</identifier><identifier>PMID: 37309514</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Chromosome 10 ; Crop production ; Disease resistance ; Exonuclease ; Gene mapping ; Genes ; Genotyping ; Inbreeding ; Life Sciences ; Mapping ; Molecular biology ; Nucleotides ; Phenotypic variations ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polymerase chain reaction ; Proteins ; Quantitative trait loci ; Rice blast ; Seedlings</subject><ispartof>Molecular breeding, 2021-12, Vol.41 (12), p.75-75, Article 75</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-cdf160e54249ca98e8d2e83a89a28075679e6c1d1baf1d73f402eab20cf016773</citedby><cites>FETCH-LOGICAL-c475t-cdf160e54249ca98e8d2e83a89a28075679e6c1d1baf1d73f402eab20cf016773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236096/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236096/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37309514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Yunyu</creatorcontrib><creatorcontrib>Xiao, Ning</creatorcontrib><creatorcontrib>Li, Yuhong</creatorcontrib><creatorcontrib>Gao, Qiang</creatorcontrib><creatorcontrib>Ning, Yuese</creatorcontrib><creatorcontrib>Yu, Ling</creatorcontrib><creatorcontrib>Cai, Yue</creatorcontrib><creatorcontrib>Pan, Cunhong</creatorcontrib><creatorcontrib>Zhang, Xiaoxiang</creatorcontrib><creatorcontrib>Huang, Niansheng</creatorcontrib><creatorcontrib>Zhou, Changhai</creatorcontrib><creatorcontrib>Ji, Hongjuan</creatorcontrib><creatorcontrib>Liu, Jianju</creatorcontrib><creatorcontrib>Shi, Wei</creatorcontrib><creatorcontrib>Chen, Zichun</creatorcontrib><creatorcontrib>Liang, Chengzhi</creatorcontrib><creatorcontrib>Li, Aihong</creatorcontrib><title>Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><addtitle>Mol Breed</addtitle><description>Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing
Pigm
into C134S and showed significant differences in the panicle blast resistance. One F
2
population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL
qPBR10-1
on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore,
qPBR10-1
was verified using the BC
1
F
2
and BC
1
F
3
population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that
ORF1
and
ORF2
encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the
qPBR10-1
locus.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chromosome 10</subject><subject>Crop production</subject><subject>Disease resistance</subject><subject>Exonuclease</subject><subject>Gene mapping</subject><subject>Genes</subject><subject>Genotyping</subject><subject>Inbreeding</subject><subject>Life Sciences</subject><subject>Mapping</subject><subject>Molecular biology</subject><subject>Nucleotides</subject><subject>Phenotypic variations</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Quantitative trait loci</subject><subject>Rice blast</subject><subject>Seedlings</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU9v1DAQxSMEoqXwBTggS1w4YDq2E9s5oVJBqVTECsrZ8jqTxZVjb-2kEhc-O162lD8HTmNpfu-Nn17TPGXwigGo48IYCE6BMwqMS03FveaQdYrTXml9v76FBipUKw6aR6VcQRX1Uj5sDoQS0HesPWy-nw8YZz96Z2efIrFxIKOPSCa73fq4IWkk16s3nxhQ9pJYEtMNBhKSWwpxKc45hbDDtjZ6F5Csgy0zyVh8mW10SHwkK7-Z6A62Pu7Y1fHl2YfPpOrwcfNgtKHgk9t51Hx59_by9D29-Hh2fnpyQV2rupm6YWQSsGt52zvba9QDRy2s7i3XoDqpepSODWxtRzYoMbbA0a45uBGYVEocNa_3vttlPeHgauZsg9lmP9n8zSTrzd-b6L-aTboxDLiQ0Mvq8OLWIafrBctsJl8chmAjpqUYrnnXgWgFVPT5P-hVWnKs-QyX0MpOtZpViu8pl1MpGce73zAwu37Nvl9T-zU_-zWiip79meNO8qvQCog9UOoqbjD_vv0f2x9EIbDT</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Wu, Yunyu</creator><creator>Xiao, Ning</creator><creator>Li, Yuhong</creator><creator>Gao, Qiang</creator><creator>Ning, Yuese</creator><creator>Yu, Ling</creator><creator>Cai, Yue</creator><creator>Pan, Cunhong</creator><creator>Zhang, Xiaoxiang</creator><creator>Huang, Niansheng</creator><creator>Zhou, Changhai</creator><creator>Ji, Hongjuan</creator><creator>Liu, Jianju</creator><creator>Shi, Wei</creator><creator>Chen, Zichun</creator><creator>Liang, Chengzhi</creator><creator>Li, Aihong</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20211201</creationdate><title>Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line</title><author>Wu, Yunyu ; Xiao, Ning ; Li, Yuhong ; Gao, Qiang ; Ning, Yuese ; Yu, Ling ; Cai, Yue ; Pan, Cunhong ; Zhang, Xiaoxiang ; Huang, Niansheng ; Zhou, Changhai ; Ji, Hongjuan ; Liu, Jianju ; Shi, Wei ; Chen, Zichun ; Liang, Chengzhi ; Li, Aihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-cdf160e54249ca98e8d2e83a89a28075679e6c1d1baf1d73f402eab20cf016773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Chromosome 10</topic><topic>Crop production</topic><topic>Disease resistance</topic><topic>Exonuclease</topic><topic>Gene mapping</topic><topic>Genes</topic><topic>Genotyping</topic><topic>Inbreeding</topic><topic>Life Sciences</topic><topic>Mapping</topic><topic>Molecular biology</topic><topic>Nucleotides</topic><topic>Phenotypic variations</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Quantitative trait loci</topic><topic>Rice blast</topic><topic>Seedlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Yunyu</creatorcontrib><creatorcontrib>Xiao, Ning</creatorcontrib><creatorcontrib>Li, Yuhong</creatorcontrib><creatorcontrib>Gao, Qiang</creatorcontrib><creatorcontrib>Ning, Yuese</creatorcontrib><creatorcontrib>Yu, Ling</creatorcontrib><creatorcontrib>Cai, Yue</creatorcontrib><creatorcontrib>Pan, Cunhong</creatorcontrib><creatorcontrib>Zhang, Xiaoxiang</creatorcontrib><creatorcontrib>Huang, Niansheng</creatorcontrib><creatorcontrib>Zhou, Changhai</creatorcontrib><creatorcontrib>Ji, Hongjuan</creatorcontrib><creatorcontrib>Liu, Jianju</creatorcontrib><creatorcontrib>Shi, Wei</creatorcontrib><creatorcontrib>Chen, Zichun</creatorcontrib><creatorcontrib>Liang, Chengzhi</creatorcontrib><creatorcontrib>Li, Aihong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Yunyu</au><au>Xiao, Ning</au><au>Li, Yuhong</au><au>Gao, Qiang</au><au>Ning, Yuese</au><au>Yu, Ling</au><au>Cai, Yue</au><au>Pan, Cunhong</au><au>Zhang, Xiaoxiang</au><au>Huang, Niansheng</au><au>Zhou, Changhai</au><au>Ji, Hongjuan</au><au>Liu, Jianju</au><au>Shi, Wei</au><au>Chen, Zichun</au><au>Liang, Chengzhi</au><au>Li, Aihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><addtitle>Mol Breed</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>41</volume><issue>12</issue><spage>75</spage><epage>75</epage><pages>75-75</pages><artnum>75</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing
Pigm
into C134S and showed significant differences in the panicle blast resistance. One F
2
population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL
qPBR10-1
on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore,
qPBR10-1
was verified using the BC
1
F
2
and BC
1
F
3
population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that
ORF1
and
ORF2
encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the
qPBR10-1
locus.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37309514</pmid><doi>10.1007/s11032-021-01268-3</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular breeding, 2021-12, Vol.41 (12), p.75-75, Article 75 |
| issn | 1380-3743 1572-9788 |
| language | eng |
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| source | PubMed (Medline); Springer Nature |
| subjects | Biomedical and Life Sciences Biotechnology Chromosome 10 Crop production Disease resistance Exonuclease Gene mapping Genes Genotyping Inbreeding Life Sciences Mapping Molecular biology Nucleotides Phenotypic variations Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polymerase chain reaction Proteins Quantitative trait loci Rice blast Seedlings |
| title | Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line |
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