CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products
Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagene...
Gespeichert in:
| Veröffentlicht in: | Molecular breeding 2021-02, Vol.41 (2), p.11-11, Article 11 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11 |
|---|---|
| container_issue | 2 |
| container_start_page | 11 |
| container_title | Molecular breeding |
| container_volume | 41 |
| creator | Nuccio, Michael L Claeys, Hannes Heyndrickx, Ken S. |
| description | Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagenesis to confirm candidate genes identified using map-based methods. Researchers are also developing tools to more effectively apply CRISPR-Cas technologies to maize because successful application of CRISPR-Cas relies on target gene identification, guide RNA development, vector design and construction, CRISPR-Cas reagent delivery to maize tissues, and plant characterization, each contributing unique challenges to CRISPR-Cas efficacy. Recent advances continue to chip away at major barriers that prevent more widespread use of CRISPR-Cas technologies in maize, including germplasm-independent delivery of CRISPR-Cas reagents and production of high-resolution genomic data in relevant germplasm to facilitate CRISPR-Cas experimental design. This has led to the development of novel breeding tools to advance maize genetics and demonstrations of how CRISPR-Cas technologies might be used to enhance maize germplasm. |
| doi_str_mv | 10.1007/s11032-021-01200-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10236071</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2484425779</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-8a70a649edda2632a2c41f48306053496ff8f89f5396420635c72f30677b62b83</originalsourceid><addsrcrecordid>eNp9kUtv1DAQxy0EoqXwBTggS1y4pIwf8YMLQiselSoVFbhieZ1Jmm7W3tpJq_32ZNm2QA-cbOn_mBn9CHnJ4JgB6LeFMRC8As4qYBygso_IIas1r6w25vH8FwYqoaU4IM9KuYQ5ZJV6Sg6EFmClFofk5-L85NvX82rhCx0xXMQ0pG5L-0hDyvEd9TTiDV3hlo6JTnFIYUU7jDj2ga5iuhmw6ZD62NCQ0Y9IY7rGgW5yaqYwlufkSeuHgi9u3yPy49PH74sv1enZ55PFh9MqSF2PlfEavJIWm8ZzJbjnQbJWGgEKaiGtalvTGtvWwirJQYk6aN7OqtZLxZdGHJH3-97NtFxjEzCO2Q9uk_u1z1uXfO_-VWJ_4bp07RhwoUCzueHNbUNOVxOW0a37EnAYfMQ0FccNr2sQQuyGvX5gvUxTjvN9jksjJa-1trOL710hp1IytvfbMHA7fm7Pz8383G9-bhd69fcd95E7YLNB7A1llmKH-c_s_9T-AtRupG4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2484425779</pqid></control><display><type>article</type><title>CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products</title><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Nuccio, Michael L ; Claeys, Hannes ; Heyndrickx, Ken S.</creator><creatorcontrib>Nuccio, Michael L ; Claeys, Hannes ; Heyndrickx, Ken S.</creatorcontrib><description>Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagenesis to confirm candidate genes identified using map-based methods. Researchers are also developing tools to more effectively apply CRISPR-Cas technologies to maize because successful application of CRISPR-Cas relies on target gene identification, guide RNA development, vector design and construction, CRISPR-Cas reagent delivery to maize tissues, and plant characterization, each contributing unique challenges to CRISPR-Cas efficacy. Recent advances continue to chip away at major barriers that prevent more widespread use of CRISPR-Cas technologies in maize, including germplasm-independent delivery of CRISPR-Cas reagents and production of high-resolution genomic data in relevant germplasm to facilitate CRISPR-Cas experimental design. This has led to the development of novel breeding tools to advance maize genetics and demonstrations of how CRISPR-Cas technologies might be used to enhance maize germplasm.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-021-01200-9</identifier><identifier>PMID: 37309473</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Corn ; CRISPR ; Design of experiments ; Experimental design ; Genetics ; Genomics and Sustainable Improvement ; Germplasm ; Life Sciences ; Maize Genetics ; Molecular biology ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Reagents ; Ribonucleic acid ; RNA ; Site-directed mutagenesis ; Target recognition</subject><ispartof>Molecular breeding, 2021-02, Vol.41 (2), p.11-11, Article 11</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-8a70a649edda2632a2c41f48306053496ff8f89f5396420635c72f30677b62b83</citedby><cites>FETCH-LOGICAL-c475t-8a70a649edda2632a2c41f48306053496ff8f89f5396420635c72f30677b62b83</cites><orcidid>0000-0002-7238-8361</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236071/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236071/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37309473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nuccio, Michael L</creatorcontrib><creatorcontrib>Claeys, Hannes</creatorcontrib><creatorcontrib>Heyndrickx, Ken S.</creatorcontrib><title>CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><addtitle>Mol Breed</addtitle><description>Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagenesis to confirm candidate genes identified using map-based methods. Researchers are also developing tools to more effectively apply CRISPR-Cas technologies to maize because successful application of CRISPR-Cas relies on target gene identification, guide RNA development, vector design and construction, CRISPR-Cas reagent delivery to maize tissues, and plant characterization, each contributing unique challenges to CRISPR-Cas efficacy. Recent advances continue to chip away at major barriers that prevent more widespread use of CRISPR-Cas technologies in maize, including germplasm-independent delivery of CRISPR-Cas reagents and production of high-resolution genomic data in relevant germplasm to facilitate CRISPR-Cas experimental design. This has led to the development of novel breeding tools to advance maize genetics and demonstrations of how CRISPR-Cas technologies might be used to enhance maize germplasm.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Corn</subject><subject>CRISPR</subject><subject>Design of experiments</subject><subject>Experimental design</subject><subject>Genetics</subject><subject>Genomics and Sustainable Improvement</subject><subject>Germplasm</subject><subject>Life Sciences</subject><subject>Maize Genetics</subject><subject>Molecular biology</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Reagents</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Site-directed mutagenesis</subject><subject>Target recognition</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kUtv1DAQxy0EoqXwBTggS1y4pIwf8YMLQiselSoVFbhieZ1Jmm7W3tpJq_32ZNm2QA-cbOn_mBn9CHnJ4JgB6LeFMRC8As4qYBygso_IIas1r6w25vH8FwYqoaU4IM9KuYQ5ZJV6Sg6EFmClFofk5-L85NvX82rhCx0xXMQ0pG5L-0hDyvEd9TTiDV3hlo6JTnFIYUU7jDj2ga5iuhmw6ZD62NCQ0Y9IY7rGgW5yaqYwlufkSeuHgi9u3yPy49PH74sv1enZ55PFh9MqSF2PlfEavJIWm8ZzJbjnQbJWGgEKaiGtalvTGtvWwirJQYk6aN7OqtZLxZdGHJH3-97NtFxjEzCO2Q9uk_u1z1uXfO_-VWJ_4bp07RhwoUCzueHNbUNOVxOW0a37EnAYfMQ0FccNr2sQQuyGvX5gvUxTjvN9jksjJa-1trOL710hp1IytvfbMHA7fm7Pz8383G9-bhd69fcd95E7YLNB7A1llmKH-c_s_9T-AtRupG4</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Nuccio, Michael L</creator><creator>Claeys, Hannes</creator><creator>Heyndrickx, Ken S.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7238-8361</orcidid></search><sort><creationdate>20210201</creationdate><title>CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products</title><author>Nuccio, Michael L ; Claeys, Hannes ; Heyndrickx, Ken S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-8a70a649edda2632a2c41f48306053496ff8f89f5396420635c72f30677b62b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Corn</topic><topic>CRISPR</topic><topic>Design of experiments</topic><topic>Experimental design</topic><topic>Genetics</topic><topic>Genomics and Sustainable Improvement</topic><topic>Germplasm</topic><topic>Life Sciences</topic><topic>Maize Genetics</topic><topic>Molecular biology</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Reagents</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Site-directed mutagenesis</topic><topic>Target recognition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nuccio, Michael L</creatorcontrib><creatorcontrib>Claeys, Hannes</creatorcontrib><creatorcontrib>Heyndrickx, Ken S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nuccio, Michael L</au><au>Claeys, Hannes</au><au>Heyndrickx, Ken S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><addtitle>Mol Breed</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>41</volume><issue>2</issue><spage>11</spage><epage>11</epage><pages>11-11</pages><artnum>11</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagenesis to confirm candidate genes identified using map-based methods. Researchers are also developing tools to more effectively apply CRISPR-Cas technologies to maize because successful application of CRISPR-Cas relies on target gene identification, guide RNA development, vector design and construction, CRISPR-Cas reagent delivery to maize tissues, and plant characterization, each contributing unique challenges to CRISPR-Cas efficacy. Recent advances continue to chip away at major barriers that prevent more widespread use of CRISPR-Cas technologies in maize, including germplasm-independent delivery of CRISPR-Cas reagents and production of high-resolution genomic data in relevant germplasm to facilitate CRISPR-Cas experimental design. This has led to the development of novel breeding tools to advance maize genetics and demonstrations of how CRISPR-Cas technologies might be used to enhance maize germplasm.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37309473</pmid><doi>10.1007/s11032-021-01200-9</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7238-8361</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1380-3743 |
| ispartof | Molecular breeding, 2021-02, Vol.41 (2), p.11-11, Article 11 |
| issn | 1380-3743 1572-9788 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10236071 |
| source | PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Biotechnology Corn CRISPR Design of experiments Experimental design Genetics Genomics and Sustainable Improvement Germplasm Life Sciences Maize Genetics Molecular biology Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Reagents Ribonucleic acid RNA Site-directed mutagenesis Target recognition |
| title | CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A46%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CRISPR-Cas%20technology%20in%20corn:%20a%20new%20key%20to%20unlock%20genetic%20knowledge%20and%20create%20novel%20products&rft.jtitle=Molecular%20breeding&rft.au=Nuccio,%20Michael%20L&rft.date=2021-02-01&rft.volume=41&rft.issue=2&rft.spage=11&rft.epage=11&rft.pages=11-11&rft.artnum=11&rft.issn=1380-3743&rft.eissn=1572-9788&rft_id=info:doi/10.1007/s11032-021-01200-9&rft_dat=%3Cproquest_pubme%3E2484425779%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2484425779&rft_id=info:pmid/37309473&rfr_iscdi=true |