GC/MS-Based Analysis of Fatty Acids and Amino Acids in H460 Cells Treated with Short-Chain and Polyunsaturated Fatty Acids: A Highly Sensitive Approach

The important metabolic characteristics of cancer cells include increased fat production and changes in amino acid metabolism. Based on the category of tumor, tumor cells are capable of synthesizing as much as 95% of saturated and monounsaturated fatty acids through de novo synthesis, even in the pr...

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Veröffentlicht in:Nutrients 2023-05, Vol.15 (10), p.2342
Hauptverfasser: Zhou, Tianxiao, Yang, Kaige, Ma, Yinjie, Huang, Jin, Fu, Wenchang, Yan, Chao, Li, Xinyan, Wang, Yan
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container_title Nutrients
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Yang, Kaige
Ma, Yinjie
Huang, Jin
Fu, Wenchang
Yan, Chao
Li, Xinyan
Wang, Yan
description The important metabolic characteristics of cancer cells include increased fat production and changes in amino acid metabolism. Based on the category of tumor, tumor cells are capable of synthesizing as much as 95% of saturated and monounsaturated fatty acids through de novo synthesis, even in the presence of sufficient dietary lipid intake. This fat transformation starts early when cell cancerization and further spread along with the tumor cells grow more malignant. In addition, local catabolism of tryptophan, a common feature, can weaken anti-tumor immunity in primary tumor lesions and TDLN. Arginine catabolism is likewise related with the inhibition of anti-tumor immunity. Due to the crucial role of amino acids in tumor growth, increasing tryptophan along with arginine catabolism will promote tumor growth. However, immune cells also require amino acids to expand and distinguish into effector cells that can kill tumor cells. Therefore, it is necessary to have a deeper understanding of the metabolism of amino acids and fatty acids within cells. In this study, we established a method for the simultaneous analysis of 64 metabolites consisting of fatty acids and amino acids, covering biosynthesis of unsaturated fatty acids, aminoacyl-tRNA biosynthesis, and fatty acid biosynthesis using the Agilent GC-MS system. We selected linoleic acid, linolenic acid, sodium acetate, and sodium butyrate to treat H460 cells to validate the current method. The differential metabolites observed in the four fatty acid groups in comparison with the control group indicate the metabolic effects of various fatty acids on H460 cells. These differential metabolites could potentially become biomarkers for the early diagnosis of lung cancer.
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In this study, we established a method for the simultaneous analysis of 64 metabolites consisting of fatty acids and amino acids, covering biosynthesis of unsaturated fatty acids, aminoacyl-tRNA biosynthesis, and fatty acid biosynthesis using the Agilent GC-MS system. We selected linoleic acid, linolenic acid, sodium acetate, and sodium butyrate to treat H460 cells to validate the current method. The differential metabolites observed in the four fatty acid groups in comparison with the control group indicate the metabolic effects of various fatty acids on H460 cells. 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subjects Acetic acid
Amino acid sequence
Amino Acids
Arginine
Biosynthesis
Catabolism
Dietary intake
Disease susceptibility
Effector cells
Energy
Esters
Fatty acids
Fatty Acids - metabolism
Fatty Acids, Unsaturated
Gas Chromatography-Mass Spectrometry
Immune system
Immunity
Laboratories
Linoleic acid
Linolenic acid
Linolenic acids
Lipids
Lung cancer
Mass spectrometry
Metabolism
Metabolites
Physiology
Polyunsaturated fatty acids
Sodium
Sodium acetate
Transfer RNA
tRNA
Tryptophan
Tumor cells
Tumors
title GC/MS-Based Analysis of Fatty Acids and Amino Acids in H460 Cells Treated with Short-Chain and Polyunsaturated Fatty Acids: A Highly Sensitive Approach
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