Screening Candidate Genes at the Co Locus Conferring to the Columnar Growth Habit in Apple ( Malus × Domestica Borkh.)
The columnar growth trait of apple ( × Borkh.) is genetically controlled by the Columnar ( ) locus on 10 chromosomes, including several candidate genes. Except for , other candidate genes at the locus are less elucidated. In this study, a strategy of step-by-step screening was adopted to select 11 c...
Gespeichert in:
| Veröffentlicht in: | Genes 2023-04, Vol.14 (5), p.964 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 5 |
| container_start_page | 964 |
| container_title | Genes |
| container_volume | 14 |
| creator | Guo, Jing Zhao, Yuan Chu, Yu Li, Yuru Song, Yuqi Pan, Qi Qiu, Zhannan Yu, Boyang Zhu, Yuandi |
| description | The columnar growth trait of apple (
×
Borkh.) is genetically controlled by the Columnar (
) locus on 10 chromosomes, including several candidate genes. Except for
, other candidate genes at the
locus are less elucidated. In this study, a strategy of step-by-step screening was adopted to select 11 candidate genes by experimental cloning, transient expression, and genetic transformation. There existed several SNPs in four genes by sequence alignment in columnar and non-columnar apples. Two genes were detected in the nucleus and three genes in the cell membrane, other genes were located in multiple cellular structures by subcellular location. Ectopic expression demonstrated that more branching occurred in
-OE by upregulating
and
and enlarged leaves in
-OE tobaccos by upregulating
. Transcripts of
and
were associated with the
genotypes in apples. The results indicate that
and
are involved in the columnar growth phenotype in apple, probably through altering polar auxin transport, active gibberellin levels, and strigolactone biosynthesis. |
| doi_str_mv | 10.3390/genes14050964 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10217585</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2820016384</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-e26cc9483224bf66d83554d8dd4496945decb3556e29a0e97aab9511acea9df93</originalsourceid><addsrcrecordid>eNpdkc1uEzEUhS0EolXpki2yxKYspvh3Ml6hEiBFCmIBrC2PfSdxmbGD7aHqk_SBeDEcNVQt3vjq3s9H9_gg9JKSc84VebuBAJkKIolqxRN0zMiCN0Iw-fRBfYROc74i9QjCCJHP0RFfMK44E8fo-ptNAMGHDV6a4LwzBfBqL4tNwWULeBnxOto51yIMkNIeLfEwGucpmIRXKV6XLb40vS_YB3yx242Az_AXM9aHf27xhzhBLt4a_D6mn9vzNy_Qs8GMGU4P9wn68enj9-Vls_66-ry8WDdW0LY0wFprleg4Y6If2tZ1XErhOueEUK0S0oHta6sFpgwBtTCmV5JSY8EoNyh-gt7d6e7mfgJnIZRkRr1LfjLpRkfj9eNJ8Fu9ib81JYwuZCerwtlBIcVfc3WhJ58tjKMJEOesWVc_lba8ExV9_R96FecUqr9K0botIaqrVHNH2RRzTjDcb0OJ3seqH8Va-VcPLdzT_0LkfwEqtJ48</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2819450098</pqid></control><display><type>article</type><title>Screening Candidate Genes at the Co Locus Conferring to the Columnar Growth Habit in Apple ( Malus × Domestica Borkh.)</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Guo, Jing ; Zhao, Yuan ; Chu, Yu ; Li, Yuru ; Song, Yuqi ; Pan, Qi ; Qiu, Zhannan ; Yu, Boyang ; Zhu, Yuandi</creator><creatorcontrib>Guo, Jing ; Zhao, Yuan ; Chu, Yu ; Li, Yuru ; Song, Yuqi ; Pan, Qi ; Qiu, Zhannan ; Yu, Boyang ; Zhu, Yuandi</creatorcontrib><description>The columnar growth trait of apple (
×
Borkh.) is genetically controlled by the Columnar (
) locus on 10 chromosomes, including several candidate genes. Except for
, other candidate genes at the
locus are less elucidated. In this study, a strategy of step-by-step screening was adopted to select 11 candidate genes by experimental cloning, transient expression, and genetic transformation. There existed several SNPs in four genes by sequence alignment in columnar and non-columnar apples. Two genes were detected in the nucleus and three genes in the cell membrane, other genes were located in multiple cellular structures by subcellular location. Ectopic expression demonstrated that more branching occurred in
-OE by upregulating
and
and enlarged leaves in
-OE tobaccos by upregulating
. Transcripts of
and
were associated with the
genotypes in apples. The results indicate that
and
are involved in the columnar growth phenotype in apple, probably through altering polar auxin transport, active gibberellin levels, and strigolactone biosynthesis.</description><identifier>ISSN: 2073-4425</identifier><identifier>EISSN: 2073-4425</identifier><identifier>DOI: 10.3390/genes14050964</identifier><identifier>PMID: 37239324</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Apples ; Artificial chromosomes ; Cell Membrane ; Cell membranes ; Cultivars ; Ectopic expression ; Gene expression ; Genetic transformation ; Genomes ; Genotypes ; Gibberellins ; Gibberellins - metabolism ; Leaves ; Localization ; Malus - metabolism ; Malus domestica ; Mutation ; Nucleotide sequence ; Phenotype ; Phenotypes ; Seeds ; Single-nucleotide polymorphism ; Sucrose ; Tobacco ; Trees</subject><ispartof>Genes, 2023-04, Vol.14 (5), p.964</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-e26cc9483224bf66d83554d8dd4496945decb3556e29a0e97aab9511acea9df93</citedby><cites>FETCH-LOGICAL-c416t-e26cc9483224bf66d83554d8dd4496945decb3556e29a0e97aab9511acea9df93</cites><orcidid>0000-0002-0093-5514</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217585/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217585/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37239324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Jing</creatorcontrib><creatorcontrib>Zhao, Yuan</creatorcontrib><creatorcontrib>Chu, Yu</creatorcontrib><creatorcontrib>Li, Yuru</creatorcontrib><creatorcontrib>Song, Yuqi</creatorcontrib><creatorcontrib>Pan, Qi</creatorcontrib><creatorcontrib>Qiu, Zhannan</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><creatorcontrib>Zhu, Yuandi</creatorcontrib><title>Screening Candidate Genes at the Co Locus Conferring to the Columnar Growth Habit in Apple ( Malus × Domestica Borkh.)</title><title>Genes</title><addtitle>Genes (Basel)</addtitle><description>The columnar growth trait of apple (
×
Borkh.) is genetically controlled by the Columnar (
) locus on 10 chromosomes, including several candidate genes. Except for
, other candidate genes at the
locus are less elucidated. In this study, a strategy of step-by-step screening was adopted to select 11 candidate genes by experimental cloning, transient expression, and genetic transformation. There existed several SNPs in four genes by sequence alignment in columnar and non-columnar apples. Two genes were detected in the nucleus and three genes in the cell membrane, other genes were located in multiple cellular structures by subcellular location. Ectopic expression demonstrated that more branching occurred in
-OE by upregulating
and
and enlarged leaves in
-OE tobaccos by upregulating
. Transcripts of
and
were associated with the
genotypes in apples. The results indicate that
and
are involved in the columnar growth phenotype in apple, probably through altering polar auxin transport, active gibberellin levels, and strigolactone biosynthesis.</description><subject>Apples</subject><subject>Artificial chromosomes</subject><subject>Cell Membrane</subject><subject>Cell membranes</subject><subject>Cultivars</subject><subject>Ectopic expression</subject><subject>Gene expression</subject><subject>Genetic transformation</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>Gibberellins</subject><subject>Gibberellins - metabolism</subject><subject>Leaves</subject><subject>Localization</subject><subject>Malus - metabolism</subject><subject>Malus domestica</subject><subject>Mutation</subject><subject>Nucleotide sequence</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Seeds</subject><subject>Single-nucleotide polymorphism</subject><subject>Sucrose</subject><subject>Tobacco</subject><subject>Trees</subject><issn>2073-4425</issn><issn>2073-4425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkc1uEzEUhS0EolXpki2yxKYspvh3Ml6hEiBFCmIBrC2PfSdxmbGD7aHqk_SBeDEcNVQt3vjq3s9H9_gg9JKSc84VebuBAJkKIolqxRN0zMiCN0Iw-fRBfYROc74i9QjCCJHP0RFfMK44E8fo-ptNAMGHDV6a4LwzBfBqL4tNwWULeBnxOto51yIMkNIeLfEwGucpmIRXKV6XLb40vS_YB3yx242Az_AXM9aHf27xhzhBLt4a_D6mn9vzNy_Qs8GMGU4P9wn68enj9-Vls_66-ry8WDdW0LY0wFprleg4Y6If2tZ1XErhOueEUK0S0oHta6sFpgwBtTCmV5JSY8EoNyh-gt7d6e7mfgJnIZRkRr1LfjLpRkfj9eNJ8Fu9ib81JYwuZCerwtlBIcVfc3WhJ58tjKMJEOesWVc_lba8ExV9_R96FecUqr9K0botIaqrVHNH2RRzTjDcb0OJ3seqH8Va-VcPLdzT_0LkfwEqtJ48</recordid><startdate>20230424</startdate><enddate>20230424</enddate><creator>Guo, Jing</creator><creator>Zhao, Yuan</creator><creator>Chu, Yu</creator><creator>Li, Yuru</creator><creator>Song, Yuqi</creator><creator>Pan, Qi</creator><creator>Qiu, Zhannan</creator><creator>Yu, Boyang</creator><creator>Zhu, Yuandi</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0093-5514</orcidid></search><sort><creationdate>20230424</creationdate><title>Screening Candidate Genes at the Co Locus Conferring to the Columnar Growth Habit in Apple ( Malus × Domestica Borkh.)</title><author>Guo, Jing ; Zhao, Yuan ; Chu, Yu ; Li, Yuru ; Song, Yuqi ; Pan, Qi ; Qiu, Zhannan ; Yu, Boyang ; Zhu, Yuandi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-e26cc9483224bf66d83554d8dd4496945decb3556e29a0e97aab9511acea9df93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Apples</topic><topic>Artificial chromosomes</topic><topic>Cell Membrane</topic><topic>Cell membranes</topic><topic>Cultivars</topic><topic>Ectopic expression</topic><topic>Gene expression</topic><topic>Genetic transformation</topic><topic>Genomes</topic><topic>Genotypes</topic><topic>Gibberellins</topic><topic>Gibberellins - metabolism</topic><topic>Leaves</topic><topic>Localization</topic><topic>Malus - metabolism</topic><topic>Malus domestica</topic><topic>Mutation</topic><topic>Nucleotide sequence</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Seeds</topic><topic>Single-nucleotide polymorphism</topic><topic>Sucrose</topic><topic>Tobacco</topic><topic>Trees</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Jing</creatorcontrib><creatorcontrib>Zhao, Yuan</creatorcontrib><creatorcontrib>Chu, Yu</creatorcontrib><creatorcontrib>Li, Yuru</creatorcontrib><creatorcontrib>Song, Yuqi</creatorcontrib><creatorcontrib>Pan, Qi</creatorcontrib><creatorcontrib>Qiu, Zhannan</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><creatorcontrib>Zhu, Yuandi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Jing</au><au>Zhao, Yuan</au><au>Chu, Yu</au><au>Li, Yuru</au><au>Song, Yuqi</au><au>Pan, Qi</au><au>Qiu, Zhannan</au><au>Yu, Boyang</au><au>Zhu, Yuandi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening Candidate Genes at the Co Locus Conferring to the Columnar Growth Habit in Apple ( Malus × Domestica Borkh.)</atitle><jtitle>Genes</jtitle><addtitle>Genes (Basel)</addtitle><date>2023-04-24</date><risdate>2023</risdate><volume>14</volume><issue>5</issue><spage>964</spage><pages>964-</pages><issn>2073-4425</issn><eissn>2073-4425</eissn><abstract>The columnar growth trait of apple (
×
Borkh.) is genetically controlled by the Columnar (
) locus on 10 chromosomes, including several candidate genes. Except for
, other candidate genes at the
locus are less elucidated. In this study, a strategy of step-by-step screening was adopted to select 11 candidate genes by experimental cloning, transient expression, and genetic transformation. There existed several SNPs in four genes by sequence alignment in columnar and non-columnar apples. Two genes were detected in the nucleus and three genes in the cell membrane, other genes were located in multiple cellular structures by subcellular location. Ectopic expression demonstrated that more branching occurred in
-OE by upregulating
and
and enlarged leaves in
-OE tobaccos by upregulating
. Transcripts of
and
were associated with the
genotypes in apples. The results indicate that
and
are involved in the columnar growth phenotype in apple, probably through altering polar auxin transport, active gibberellin levels, and strigolactone biosynthesis.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37239324</pmid><doi>10.3390/genes14050964</doi><orcidid>https://orcid.org/0000-0002-0093-5514</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2073-4425 |
| ispartof | Genes, 2023-04, Vol.14 (5), p.964 |
| issn | 2073-4425 2073-4425 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10217585 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Apples Artificial chromosomes Cell Membrane Cell membranes Cultivars Ectopic expression Gene expression Genetic transformation Genomes Genotypes Gibberellins Gibberellins - metabolism Leaves Localization Malus - metabolism Malus domestica Mutation Nucleotide sequence Phenotype Phenotypes Seeds Single-nucleotide polymorphism Sucrose Tobacco Trees |
| title | Screening Candidate Genes at the Co Locus Conferring to the Columnar Growth Habit in Apple ( Malus × Domestica Borkh.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T20%3A13%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Screening%20Candidate%20Genes%20at%20the%20Co%20Locus%20Conferring%20to%20the%20Columnar%20Growth%20Habit%20in%20Apple%20(%20Malus%20%C3%97%20Domestica%20Borkh.)&rft.jtitle=Genes&rft.au=Guo,%20Jing&rft.date=2023-04-24&rft.volume=14&rft.issue=5&rft.spage=964&rft.pages=964-&rft.issn=2073-4425&rft.eissn=2073-4425&rft_id=info:doi/10.3390/genes14050964&rft_dat=%3Cproquest_pubme%3E2820016384%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2819450098&rft_id=info:pmid/37239324&rfr_iscdi=true |