Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex
Coenzyme Q10 is an important component of the mitochondrial electron transfer chain. A supercomplex of mitochondrial electron transfer system proteins exists. This complex also contains coenzyme Q10. The concentrations of coenzyme Q10 in tissues decrease with age and pathology. Coenzyme Q10 is given...
Gespeichert in:
| Veröffentlicht in: | Journal of Clinical Biochemistry and Nutrition 2023, Vol.72(3), pp.207-214 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 3 |
| container_start_page | 207 |
| container_title | Journal of Clinical Biochemistry and Nutrition |
| container_volume | 72 |
| creator | Sugawara, Kyousuke Sato, Seiji Tanaka, Yuto Nakamura, Akari Fujisawa, Akio Yamamoto, Yorihiro Kashiba, Misato |
| description | Coenzyme Q10 is an important component of the mitochondrial electron transfer chain. A supercomplex of mitochondrial electron transfer system proteins exists. This complex also contains coenzyme Q10. The concentrations of coenzyme Q10 in tissues decrease with age and pathology. Coenzyme Q10 is given as a supplement. It is unknown whether coenzyme Q10 is transported to the supercomplex. We develop a method for measuring coenzyme Q10 in the mitochondrial respiratory chain supercomplex in this study. Blue native electrophoresis was used to separate mitochondrial membranes. Electrophoresis gels were cut into 3 mm slices. Hexane was used to extract coenzyme Q10 from this slice, and HPLC-ECD was used to analyze coenzyme Q10. Coenzyme Q10 was found in the gel at the same site as the supercomplex. Coenzyme Q10 at this location was thought to be coenzyme Q10 in the supercomplex. We discovered that 4-nitrobenzoate, a coenzyme Q10 biosynthesis inhibitor, reduced the amount of coenzyme Q10 both within and outside the supercomplex. We also observed that the addition of coenzyme Q10 to cells increased the amount of coenzyme Q10 in the supercomplex. It is expected to analysis coenzyme Q10 level in supercomplex in various samples by using this novel method. |
| doi_str_mv | 10.3164/jcbn.22-137 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10209600</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2837253573</sourcerecordid><originalsourceid>FETCH-LOGICAL-c615t-57490b59776056ad31d0bcfc40d4e91587cfe8a27f3f4c33e9a2440c19cb5f523</originalsourceid><addsrcrecordid>eNpd0c2L1DAYBvAgijuunrxLwYsgXd8kTdOeZBn8ghUR9CaENH07zdAmNUkX9783Q9dBvSSE_HjIm4eQ5xSuOK2rN0fTuSvGSsrlA7KjTQOlgKZ-SHbQUlYCQHtBnsR4BKhqUVePyQWXTNC2Zjvy4zOm0ffF4EPRY0KTrDsUe_-VQmGd8WHxQSfrXT4VacRitsmb0bs-WD0VAeNiM_DhrjCjziauCwbj52XCX0_Jo0FPEZ_d75fk-_t33_Yfy5svHz7tr29KU1ORSiGrFjrRSlmDqHXPaQ-dGUwFfYUtFY00AzaayYEPleEcW82qCgxtTScGwfglebvlLms3Y2_QpaAntQQ763CnvLbq3xtnR3Xwt4oCg7YGyAmv7hOC_7liTGq20eA0aYd-jYo1GUqavzXTl__Ro1-Dy_NldfpYLiTP6vWmTPAxBhzOr6GgTrWpU22KMZVry_rF3wOc7Z-eMrjewDEmfcAz0CFZM-EWJpnip2ULPd_lXoJCx38D5bOsWA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2837253573</pqid></control><display><type>article</type><title>Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex</title><source>J-STAGE Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Sugawara, Kyousuke ; Sato, Seiji ; Tanaka, Yuto ; Nakamura, Akari ; Fujisawa, Akio ; Yamamoto, Yorihiro ; Kashiba, Misato</creator><creatorcontrib>Sugawara, Kyousuke ; Sato, Seiji ; Tanaka, Yuto ; Nakamura, Akari ; Fujisawa, Akio ; Yamamoto, Yorihiro ; Kashiba, Misato</creatorcontrib><description>Coenzyme Q10 is an important component of the mitochondrial electron transfer chain. A supercomplex of mitochondrial electron transfer system proteins exists. This complex also contains coenzyme Q10. The concentrations of coenzyme Q10 in tissues decrease with age and pathology. Coenzyme Q10 is given as a supplement. It is unknown whether coenzyme Q10 is transported to the supercomplex. We develop a method for measuring coenzyme Q10 in the mitochondrial respiratory chain supercomplex in this study. Blue native electrophoresis was used to separate mitochondrial membranes. Electrophoresis gels were cut into 3 mm slices. Hexane was used to extract coenzyme Q10 from this slice, and HPLC-ECD was used to analyze coenzyme Q10. Coenzyme Q10 was found in the gel at the same site as the supercomplex. Coenzyme Q10 at this location was thought to be coenzyme Q10 in the supercomplex. We discovered that 4-nitrobenzoate, a coenzyme Q10 biosynthesis inhibitor, reduced the amount of coenzyme Q10 both within and outside the supercomplex. We also observed that the addition of coenzyme Q10 to cells increased the amount of coenzyme Q10 in the supercomplex. It is expected to analysis coenzyme Q10 level in supercomplex in various samples by using this novel method.</description><identifier>ISSN: 0912-0009</identifier><identifier>EISSN: 1880-5086</identifier><identifier>DOI: 10.3164/jcbn.22-137</identifier><identifier>PMID: 37251962</identifier><language>eng</language><publisher>Japan: SOCIETY FOR FREE RADICAL RESEARCH JAPAN</publisher><subject>Biosynthesis ; blue native electrophoresis ; Coenzyme Q10 ; Electron transfer ; Electron transport ; Electrons ; Electrophoresis ; Gels ; Hexanes ; HPLC-ECD ; Measurement methods ; Mitochondria ; Original ; respiratory chain supercomplex</subject><ispartof>Journal of Clinical Biochemistry and Nutrition, 2023, Vol.72(3), pp.207-214</ispartof><rights>2023 JCBN</rights><rights>Copyright © 2023 JCBN.</rights><rights>2023. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2023 JCBN 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c615t-57490b59776056ad31d0bcfc40d4e91587cfe8a27f3f4c33e9a2440c19cb5f523</citedby><cites>FETCH-LOGICAL-c615t-57490b59776056ad31d0bcfc40d4e91587cfe8a27f3f4c33e9a2440c19cb5f523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209600/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209600/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37251962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugawara, Kyousuke</creatorcontrib><creatorcontrib>Sato, Seiji</creatorcontrib><creatorcontrib>Tanaka, Yuto</creatorcontrib><creatorcontrib>Nakamura, Akari</creatorcontrib><creatorcontrib>Fujisawa, Akio</creatorcontrib><creatorcontrib>Yamamoto, Yorihiro</creatorcontrib><creatorcontrib>Kashiba, Misato</creatorcontrib><title>Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex</title><title>Journal of Clinical Biochemistry and Nutrition</title><addtitle>J. Clin. Biochem. Nutr.</addtitle><description>Coenzyme Q10 is an important component of the mitochondrial electron transfer chain. A supercomplex of mitochondrial electron transfer system proteins exists. This complex also contains coenzyme Q10. The concentrations of coenzyme Q10 in tissues decrease with age and pathology. Coenzyme Q10 is given as a supplement. It is unknown whether coenzyme Q10 is transported to the supercomplex. We develop a method for measuring coenzyme Q10 in the mitochondrial respiratory chain supercomplex in this study. Blue native electrophoresis was used to separate mitochondrial membranes. Electrophoresis gels were cut into 3 mm slices. Hexane was used to extract coenzyme Q10 from this slice, and HPLC-ECD was used to analyze coenzyme Q10. Coenzyme Q10 was found in the gel at the same site as the supercomplex. Coenzyme Q10 at this location was thought to be coenzyme Q10 in the supercomplex. We discovered that 4-nitrobenzoate, a coenzyme Q10 biosynthesis inhibitor, reduced the amount of coenzyme Q10 both within and outside the supercomplex. We also observed that the addition of coenzyme Q10 to cells increased the amount of coenzyme Q10 in the supercomplex. It is expected to analysis coenzyme Q10 level in supercomplex in various samples by using this novel method.</description><subject>Biosynthesis</subject><subject>blue native electrophoresis</subject><subject>Coenzyme Q10</subject><subject>Electron transfer</subject><subject>Electron transport</subject><subject>Electrons</subject><subject>Electrophoresis</subject><subject>Gels</subject><subject>Hexanes</subject><subject>HPLC-ECD</subject><subject>Measurement methods</subject><subject>Mitochondria</subject><subject>Original</subject><subject>respiratory chain supercomplex</subject><issn>0912-0009</issn><issn>1880-5086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpd0c2L1DAYBvAgijuunrxLwYsgXd8kTdOeZBn8ghUR9CaENH07zdAmNUkX9783Q9dBvSSE_HjIm4eQ5xSuOK2rN0fTuSvGSsrlA7KjTQOlgKZ-SHbQUlYCQHtBnsR4BKhqUVePyQWXTNC2Zjvy4zOm0ffF4EPRY0KTrDsUe_-VQmGd8WHxQSfrXT4VacRitsmb0bs-WD0VAeNiM_DhrjCjziauCwbj52XCX0_Jo0FPEZ_d75fk-_t33_Yfy5svHz7tr29KU1ORSiGrFjrRSlmDqHXPaQ-dGUwFfYUtFY00AzaayYEPleEcW82qCgxtTScGwfglebvlLms3Y2_QpaAntQQ763CnvLbq3xtnR3Xwt4oCg7YGyAmv7hOC_7liTGq20eA0aYd-jYo1GUqavzXTl__Ro1-Dy_NldfpYLiTP6vWmTPAxBhzOr6GgTrWpU22KMZVry_rF3wOc7Z-eMrjewDEmfcAz0CFZM-EWJpnip2ULPd_lXoJCx38D5bOsWA</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Sugawara, Kyousuke</creator><creator>Sato, Seiji</creator><creator>Tanaka, Yuto</creator><creator>Nakamura, Akari</creator><creator>Fujisawa, Akio</creator><creator>Yamamoto, Yorihiro</creator><creator>Kashiba, Misato</creator><general>SOCIETY FOR FREE RADICAL RESEARCH JAPAN</general><general>Japan Science and Technology Agency</general><general>the Society for Free Radical Research Japan</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230101</creationdate><title>Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex</title><author>Sugawara, Kyousuke ; Sato, Seiji ; Tanaka, Yuto ; Nakamura, Akari ; Fujisawa, Akio ; Yamamoto, Yorihiro ; Kashiba, Misato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c615t-57490b59776056ad31d0bcfc40d4e91587cfe8a27f3f4c33e9a2440c19cb5f523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biosynthesis</topic><topic>blue native electrophoresis</topic><topic>Coenzyme Q10</topic><topic>Electron transfer</topic><topic>Electron transport</topic><topic>Electrons</topic><topic>Electrophoresis</topic><topic>Gels</topic><topic>Hexanes</topic><topic>HPLC-ECD</topic><topic>Measurement methods</topic><topic>Mitochondria</topic><topic>Original</topic><topic>respiratory chain supercomplex</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugawara, Kyousuke</creatorcontrib><creatorcontrib>Sato, Seiji</creatorcontrib><creatorcontrib>Tanaka, Yuto</creatorcontrib><creatorcontrib>Nakamura, Akari</creatorcontrib><creatorcontrib>Fujisawa, Akio</creatorcontrib><creatorcontrib>Yamamoto, Yorihiro</creatorcontrib><creatorcontrib>Kashiba, Misato</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Biochemistry and Nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugawara, Kyousuke</au><au>Sato, Seiji</au><au>Tanaka, Yuto</au><au>Nakamura, Akari</au><au>Fujisawa, Akio</au><au>Yamamoto, Yorihiro</au><au>Kashiba, Misato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex</atitle><jtitle>Journal of Clinical Biochemistry and Nutrition</jtitle><addtitle>J. Clin. Biochem. Nutr.</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>72</volume><issue>3</issue><spage>207</spage><epage>214</epage><pages>207-214</pages><artnum>22-137</artnum><issn>0912-0009</issn><eissn>1880-5086</eissn><abstract>Coenzyme Q10 is an important component of the mitochondrial electron transfer chain. A supercomplex of mitochondrial electron transfer system proteins exists. This complex also contains coenzyme Q10. The concentrations of coenzyme Q10 in tissues decrease with age and pathology. Coenzyme Q10 is given as a supplement. It is unknown whether coenzyme Q10 is transported to the supercomplex. We develop a method for measuring coenzyme Q10 in the mitochondrial respiratory chain supercomplex in this study. Blue native electrophoresis was used to separate mitochondrial membranes. Electrophoresis gels were cut into 3 mm slices. Hexane was used to extract coenzyme Q10 from this slice, and HPLC-ECD was used to analyze coenzyme Q10. Coenzyme Q10 was found in the gel at the same site as the supercomplex. Coenzyme Q10 at this location was thought to be coenzyme Q10 in the supercomplex. We discovered that 4-nitrobenzoate, a coenzyme Q10 biosynthesis inhibitor, reduced the amount of coenzyme Q10 both within and outside the supercomplex. We also observed that the addition of coenzyme Q10 to cells increased the amount of coenzyme Q10 in the supercomplex. It is expected to analysis coenzyme Q10 level in supercomplex in various samples by using this novel method.</abstract><cop>Japan</cop><pub>SOCIETY FOR FREE RADICAL RESEARCH JAPAN</pub><pmid>37251962</pmid><doi>10.3164/jcbn.22-137</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0912-0009 |
| ispartof | Journal of Clinical Biochemistry and Nutrition, 2023, Vol.72(3), pp.207-214 |
| issn | 0912-0009 1880-5086 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10209600 |
| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biosynthesis blue native electrophoresis Coenzyme Q10 Electron transfer Electron transport Electrons Electrophoresis Gels Hexanes HPLC-ECD Measurement methods Mitochondria Original respiratory chain supercomplex |
| title | Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T12%3A56%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Method%20for%20detecting%20CoQ10%20incorporation%20in%20the%20mitochondrial%20respiratory%20chain%20supercomplex&rft.jtitle=Journal%20of%20Clinical%20Biochemistry%20and%20Nutrition&rft.au=Sugawara,%20Kyousuke&rft.date=2023-01-01&rft.volume=72&rft.issue=3&rft.spage=207&rft.epage=214&rft.pages=207-214&rft.artnum=22-137&rft.issn=0912-0009&rft.eissn=1880-5086&rft_id=info:doi/10.3164/jcbn.22-137&rft_dat=%3Cproquest_pubme%3E2837253573%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2837253573&rft_id=info:pmid/37251962&rfr_iscdi=true |