Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection
Foot-and-mouth disease (FMD) is endemic in India with a majority of outbreaks caused by FMD virus (FMDV) serotype O. In the present study a panel of eight (2F9, 2G10, 3B9, 3H5, 4C8, 4D6, 4G10 and 5B6) mouse monoclonal antibodies (MAbs) were developed against FMDV serotype O Indian vaccine strain, O/...
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| Veröffentlicht in: | Veterinary research communications 2023-12, Vol.47 (4), p.1915-1924 |
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| creator | Mallick, Smrutirekha Singh, Rabindra Prasad Biswal, Jitendra Kumar Mohapatra, Jajati Keshari Rout, Manoranjan Samanta, Reshma Khulape, Sagar Ashok Ranjan, Rajeev |
| description | Foot-and-mouth disease (FMD) is endemic in India with a majority of outbreaks caused by FMD virus (FMDV) serotype O. In the present study a panel of eight (2F9, 2G10, 3B9, 3H5, 4C8, 4D6, 4G10 and 5B6) mouse monoclonal antibodies (MAbs) were developed against FMDV serotype O Indian vaccine strain, O/IND/R2/75 via hybridoma systems. The MAbs generated were FMDV/O specific without cross-reactivity against FMDV type A and Asia 1. All the MAbs were identified as IgG1/kappa type. Out of eight, three MAbs (3B9, 3H5 and 4G10) demonstrated virus neutralizing activity. The reactivity of all MAbs increased with heat treated (@56
0
C) serotype O antigen compared to untreated antigen in sandwich ELISA indicating that their binding epitopes are linear. Six MAbs (except 2F9 and 4D6) reacted with recombinant P1 protein of homologous virus in an indirect ELISA among which only MAb 3B9 bound to VP1. MAb profiling of 37 serotype O field viruses isolated between the years 1962 and 2021 demonstrated antigenic similarity between field isolates and reference vaccine strain. MAbs 5B6 and 4C8 consistently reacted with all 37 isolates. In indirect immunofluorescence assay MAb 5B6 bound well with FMDV/O antigen. Finally, a sandwich ELISA was successfully developed using rabbit polyclonal anti-FMDV/O serum and MAb 5B6 for detection of FMDV/O antigen in clinical samples (
n
= 649). The new assay exhibited 100% and 98.89% diagnostic sensitivity and specificity respectively compared to traditional polyclonal antibody-based sandwich ELISA suggesting that the MAb-based ELISA developed here could be an effective method for detection of FMDV serotype O. |
| doi_str_mv | 10.1007/s11259-023-10143-9 |
| format | Article |
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0
C) serotype O antigen compared to untreated antigen in sandwich ELISA indicating that their binding epitopes are linear. Six MAbs (except 2F9 and 4D6) reacted with recombinant P1 protein of homologous virus in an indirect ELISA among which only MAb 3B9 bound to VP1. MAb profiling of 37 serotype O field viruses isolated between the years 1962 and 2021 demonstrated antigenic similarity between field isolates and reference vaccine strain. MAbs 5B6 and 4C8 consistently reacted with all 37 isolates. In indirect immunofluorescence assay MAb 5B6 bound well with FMDV/O antigen. Finally, a sandwich ELISA was successfully developed using rabbit polyclonal anti-FMDV/O serum and MAb 5B6 for detection of FMDV/O antigen in clinical samples (
n
= 649). The new assay exhibited 100% and 98.89% diagnostic sensitivity and specificity respectively compared to traditional polyclonal antibody-based sandwich ELISA suggesting that the MAb-based ELISA developed here could be an effective method for detection of FMDV serotype O.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-023-10143-9</identifier><identifier>PMID: 37222940</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Antibodies, Monoclonal ; Antibodies, Viral ; Antigens ; Biomedical and Life Sciences ; Cross-reactivity ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - veterinary ; Epitopes ; Foot & mouth disease ; Foot-and-Mouth Disease - diagnosis ; Foot-and-Mouth Disease Virus ; Immunofluorescence ; Immunoglobulin G ; Life Sciences ; Mice ; Monoclonal antibodies ; O antigen ; O Antigens ; p1 Protein ; Polyclonal antibodies ; Rabbits ; Serogroup ; Vaccines ; Veterinary Medicine/Veterinary Science ; Viruses ; VP1 protein ; Zoology</subject><ispartof>Veterinary research communications, 2023-12, Vol.47 (4), p.1915-1924</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-9bfcfc8edb9b95fb244fec5cdae58d996666cf9a4ce04c03cc8b0621e707c55b3</citedby><cites>FETCH-LOGICAL-c475t-9bfcfc8edb9b95fb244fec5cdae58d996666cf9a4ce04c03cc8b0621e707c55b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-023-10143-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-023-10143-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,315,782,786,887,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37222940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mallick, Smrutirekha</creatorcontrib><creatorcontrib>Singh, Rabindra Prasad</creatorcontrib><creatorcontrib>Biswal, Jitendra Kumar</creatorcontrib><creatorcontrib>Mohapatra, Jajati Keshari</creatorcontrib><creatorcontrib>Rout, Manoranjan</creatorcontrib><creatorcontrib>Samanta, Reshma</creatorcontrib><creatorcontrib>Khulape, Sagar Ashok</creatorcontrib><creatorcontrib>Ranjan, Rajeev</creatorcontrib><title>Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Foot-and-mouth disease (FMD) is endemic in India with a majority of outbreaks caused by FMD virus (FMDV) serotype O. In the present study a panel of eight (2F9, 2G10, 3B9, 3H5, 4C8, 4D6, 4G10 and 5B6) mouse monoclonal antibodies (MAbs) were developed against FMDV serotype O Indian vaccine strain, O/IND/R2/75 via hybridoma systems. The MAbs generated were FMDV/O specific without cross-reactivity against FMDV type A and Asia 1. All the MAbs were identified as IgG1/kappa type. Out of eight, three MAbs (3B9, 3H5 and 4G10) demonstrated virus neutralizing activity. The reactivity of all MAbs increased with heat treated (@56
0
C) serotype O antigen compared to untreated antigen in sandwich ELISA indicating that their binding epitopes are linear. Six MAbs (except 2F9 and 4D6) reacted with recombinant P1 protein of homologous virus in an indirect ELISA among which only MAb 3B9 bound to VP1. MAb profiling of 37 serotype O field viruses isolated between the years 1962 and 2021 demonstrated antigenic similarity between field isolates and reference vaccine strain. MAbs 5B6 and 4C8 consistently reacted with all 37 isolates. In indirect immunofluorescence assay MAb 5B6 bound well with FMDV/O antigen. Finally, a sandwich ELISA was successfully developed using rabbit polyclonal anti-FMDV/O serum and MAb 5B6 for detection of FMDV/O antigen in clinical samples (
n
= 649). The new assay exhibited 100% and 98.89% diagnostic sensitivity and specificity respectively compared to traditional polyclonal antibody-based sandwich ELISA suggesting that the MAb-based ELISA developed here could be an effective method for detection of FMDV serotype O.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibodies, Viral</subject><subject>Antigens</subject><subject>Biomedical and Life Sciences</subject><subject>Cross-reactivity</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Epitopes</subject><subject>Foot & mouth disease</subject><subject>Foot-and-Mouth Disease - diagnosis</subject><subject>Foot-and-Mouth Disease Virus</subject><subject>Immunofluorescence</subject><subject>Immunoglobulin G</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>O antigen</subject><subject>O Antigens</subject><subject>p1 Protein</subject><subject>Polyclonal antibodies</subject><subject>Rabbits</subject><subject>Serogroup</subject><subject>Vaccines</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Viruses</subject><subject>VP1 protein</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kk1v1DAQhiMEokvhD3BAlrhwMYw_so5PqKoKVFqpSMDZcpzJrqskXmxnUflF_Ey8H5SPA75Ymnned8aeqarnDF4zAPUmMcZrTYELyoBJQfWDasFqJaiScvmwWgBb1lSJBs6qJyndAoBuQDyuzoTinGsJi-rHxxi62WUfJmKnjriNjdZljP67PQRDT8YwBTeEyQ4Fyb4NncdE7Nr6KWXSh5BpkdIxzHlDOp_QJiQ7H-dEEsaQ77ZIbg7uHe5wCNsRp7w3tiSV6DfvNuRqdf3ponjFk3BfaI1TUWQ8dPe0etTbIeGz031efXl39fnyA13dvL--vFhRJ1WdqW5717sGu1a3uu5bLmWPrnadxbrptF6W43ptpUOQDoRzTQtLzlCBcnXdivPq7dF3O7cjdq60Gu1gttGPNt6ZYL35OzP5jVmHnWHAYSkkFIdXJ4cYvs6Yshl9cjgMdsIwJ8Mb1iipQPCCvvwHvQ1zLB-9p3RTZqm4KhQ_Ui6GlCL2990wMPtNMMdNMGUTzGETjC6iF3--417ya_QFEEcgldS0xvi79n9sfwLi08RX</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Mallick, Smrutirekha</creator><creator>Singh, Rabindra Prasad</creator><creator>Biswal, Jitendra Kumar</creator><creator>Mohapatra, Jajati Keshari</creator><creator>Rout, Manoranjan</creator><creator>Samanta, Reshma</creator><creator>Khulape, Sagar Ashok</creator><creator>Ranjan, Rajeev</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20231201</creationdate><title>Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection</title><author>Mallick, Smrutirekha ; Singh, Rabindra Prasad ; Biswal, Jitendra Kumar ; Mohapatra, Jajati Keshari ; Rout, Manoranjan ; Samanta, Reshma ; Khulape, Sagar Ashok ; Ranjan, Rajeev</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-9bfcfc8edb9b95fb244fec5cdae58d996666cf9a4ce04c03cc8b0621e707c55b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibodies, Viral</topic><topic>Antigens</topic><topic>Biomedical and Life Sciences</topic><topic>Cross-reactivity</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Epitopes</topic><topic>Foot & mouth disease</topic><topic>Foot-and-Mouth Disease - diagnosis</topic><topic>Foot-and-Mouth Disease Virus</topic><topic>Immunofluorescence</topic><topic>Immunoglobulin G</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>O antigen</topic><topic>O Antigens</topic><topic>p1 Protein</topic><topic>Polyclonal antibodies</topic><topic>Rabbits</topic><topic>Serogroup</topic><topic>Vaccines</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Viruses</topic><topic>VP1 protein</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mallick, Smrutirekha</creatorcontrib><creatorcontrib>Singh, Rabindra Prasad</creatorcontrib><creatorcontrib>Biswal, Jitendra Kumar</creatorcontrib><creatorcontrib>Mohapatra, Jajati Keshari</creatorcontrib><creatorcontrib>Rout, Manoranjan</creatorcontrib><creatorcontrib>Samanta, Reshma</creatorcontrib><creatorcontrib>Khulape, Sagar Ashok</creatorcontrib><creatorcontrib>Ranjan, Rajeev</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mallick, Smrutirekha</au><au>Singh, Rabindra Prasad</au><au>Biswal, Jitendra Kumar</au><au>Mohapatra, Jajati Keshari</au><au>Rout, Manoranjan</au><au>Samanta, Reshma</au><au>Khulape, Sagar Ashok</au><au>Ranjan, Rajeev</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>47</volume><issue>4</issue><spage>1915</spage><epage>1924</epage><pages>1915-1924</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>Foot-and-mouth disease (FMD) is endemic in India with a majority of outbreaks caused by FMD virus (FMDV) serotype O. In the present study a panel of eight (2F9, 2G10, 3B9, 3H5, 4C8, 4D6, 4G10 and 5B6) mouse monoclonal antibodies (MAbs) were developed against FMDV serotype O Indian vaccine strain, O/IND/R2/75 via hybridoma systems. The MAbs generated were FMDV/O specific without cross-reactivity against FMDV type A and Asia 1. All the MAbs were identified as IgG1/kappa type. Out of eight, three MAbs (3B9, 3H5 and 4G10) demonstrated virus neutralizing activity. The reactivity of all MAbs increased with heat treated (@56
0
C) serotype O antigen compared to untreated antigen in sandwich ELISA indicating that their binding epitopes are linear. Six MAbs (except 2F9 and 4D6) reacted with recombinant P1 protein of homologous virus in an indirect ELISA among which only MAb 3B9 bound to VP1. MAb profiling of 37 serotype O field viruses isolated between the years 1962 and 2021 demonstrated antigenic similarity between field isolates and reference vaccine strain. MAbs 5B6 and 4C8 consistently reacted with all 37 isolates. In indirect immunofluorescence assay MAb 5B6 bound well with FMDV/O antigen. Finally, a sandwich ELISA was successfully developed using rabbit polyclonal anti-FMDV/O serum and MAb 5B6 for detection of FMDV/O antigen in clinical samples (
n
= 649). The new assay exhibited 100% and 98.89% diagnostic sensitivity and specificity respectively compared to traditional polyclonal antibody-based sandwich ELISA suggesting that the MAb-based ELISA developed here could be an effective method for detection of FMDV serotype O.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37222940</pmid><doi>10.1007/s11259-023-10143-9</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-7380 |
| ispartof | Veterinary research communications, 2023-12, Vol.47 (4), p.1915-1924 |
| issn | 0165-7380 1573-7446 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10206340 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Antibodies, Monoclonal Antibodies, Viral Antigens Biomedical and Life Sciences Cross-reactivity Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - veterinary Epitopes Foot & mouth disease Foot-and-Mouth Disease - diagnosis Foot-and-Mouth Disease Virus Immunofluorescence Immunoglobulin G Life Sciences Mice Monoclonal antibodies O antigen O Antigens p1 Protein Polyclonal antibodies Rabbits Serogroup Vaccines Veterinary Medicine/Veterinary Science Viruses VP1 protein Zoology |
| title | Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection |
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