Treasure from trash - Is Ecoenzyme the new panacea in conservative dentistry and endodontics?
Context: Endodontic biofilm eradication is achieved by chemo-mechanical disinfection. The search for a safer, nontoxic irrigant led us to a natural product, Ecoenzyme. Aim: This study aims to analyze Ecoenzyme (EE) and explore its antimicrobial and biofilm disrupting activity against a 1-week mature...
Gespeichert in:
| Veröffentlicht in: | Journal of conservative dentistry 2023-03, Vol.26 (2), p.176-181 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 181 |
|---|---|
| container_issue | 2 |
| container_start_page | 176 |
| container_title | Journal of conservative dentistry |
| container_volume | 26 |
| creator | Sai, Shamini J. Abisha, V Mahalakshmi, Krishnan Veronica, Aruna Susila, Anand |
| description | Context: Endodontic biofilm eradication is achieved by chemo-mechanical disinfection. The search for a safer, nontoxic irrigant led us to a natural product, Ecoenzyme.
Aim: This study aims to analyze Ecoenzyme (EE) and explore its antimicrobial and biofilm disrupting activity against a 1-week mature multi-species biofilm.
Materials and Methods: Qualitative assessment of the phytochemicals present in EE was conducted. Minimal inhibitory concentration (MIC), minimum bactericidal concentration, and zone of inhibition (ZOI) were recorded. Multi-species biofilm of Streptococcus mutans (MTCC 497), Lactobacillus acidophilus (MTCC 10307), and Enterococcus faecalis (ATCC 29212) was grown and time-kill assay was performed to test biofilm disruption for EE, 3.5% sodium hypochlorite (NaOCl) (control). Student's t-test and one-way ANOVA with post hoc analysis were conducted for ZOI and time-kill assay, respectively. Statistical significance was set at P ≤ 0.05.
Results: EE contained secondary metabolites having antibacterial properties. MIC was 25% (S. mutans), 50% (E. faecalis), and >50% (L. acidophilus). EE disrupted ~90% of biofilm species in 5 min of exposure while NaOCl achieved ~99.9% reduction. Further reduction by EE progressed over 20 min after which no viable bacteria in the biofilm was cultivable.
Conclusions: Lemon peel Ecoenzyme (EE) is antimicrobial with effective biofilm-disrupting properties on a mature multi-species biofilm. However, its effects were slower than 3.5% sodium hypochlorite. |
| doi_str_mv | 10.4103/jcd.jcd_473_22 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10190087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A746003471</galeid><sourcerecordid>A746003471</sourcerecordid><originalsourceid>FETCH-LOGICAL-g365e-6ad4becc3eb6f0bdad8faf23cc5db2269b362fd410658ee023a9b79181fe253f3</originalsourceid><addsrcrecordid>eNptksFvFCEUxidGY2v16tGQmBgvszLAwMypNk3VJk281KMhDDx22M7ACjO7Wf96WdvqrjGEQB6_98H7eEXxusILVmH6YaXNIk_JBJWEPClOcStYWRNMn_7ekxILLE6KFymtMOaMtfh5cUIFwXXTstPi-20EleYIyMYwoimq1KMSXSd0pQP4n7sR0NQD8rBFa-WVBoWcRzr4BHGjJrcBZMBPLk1xh5Q3CLwJJuSITucvi2dWDQlePaxnxbdPV7eXX8qbr5-vLy9uyiXlNZRcGdaB1hQ6bnFnlGmssoRqXZuOEN52lBNrcsG8bgAwoartRFs1lQVSU0vPivN73fXcjWB0flBUg1xHN6q4k0E5eXziXS-XYSMrXLUYNyIrvH9QiOHHDGmSo0sahkF5CHOSpKm44KLmNKNv_0FXYY4-1yeJaBlrsu0H1FINIJ23IV-s96LyQjCOMWWiytTiP1QeBkaXTQbrcvwo4d1BQg9qmPoUhnly-UeOwTeHlvzx4vHrM_DxHtiGYYKY7oZ5C1Fm9s6HbXZG7htM7pvrb4PJSnD52DL0F6M1xxc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2794480973</pqid></control><display><type>article</type><title>Treasure from trash - Is Ecoenzyme the new panacea in conservative dentistry and endodontics?</title><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Sai, Shamini ; J. Abisha, V ; Mahalakshmi, Krishnan ; Veronica, Aruna ; Susila, Anand</creator><creatorcontrib>Sai, Shamini ; J. Abisha, V ; Mahalakshmi, Krishnan ; Veronica, Aruna ; Susila, Anand</creatorcontrib><description>Context: Endodontic biofilm eradication is achieved by chemo-mechanical disinfection. The search for a safer, nontoxic irrigant led us to a natural product, Ecoenzyme.
Aim: This study aims to analyze Ecoenzyme (EE) and explore its antimicrobial and biofilm disrupting activity against a 1-week mature multi-species biofilm.
Materials and Methods: Qualitative assessment of the phytochemicals present in EE was conducted. Minimal inhibitory concentration (MIC), minimum bactericidal concentration, and zone of inhibition (ZOI) were recorded. Multi-species biofilm of Streptococcus mutans (MTCC 497), Lactobacillus acidophilus (MTCC 10307), and Enterococcus faecalis (ATCC 29212) was grown and time-kill assay was performed to test biofilm disruption for EE, 3.5% sodium hypochlorite (NaOCl) (control). Student's t-test and one-way ANOVA with post hoc analysis were conducted for ZOI and time-kill assay, respectively. Statistical significance was set at P ≤ 0.05.
Results: EE contained secondary metabolites having antibacterial properties. MIC was 25% (S. mutans), 50% (E. faecalis), and >50% (L. acidophilus). EE disrupted ~90% of biofilm species in 5 min of exposure while NaOCl achieved ~99.9% reduction. Further reduction by EE progressed over 20 min after which no viable bacteria in the biofilm was cultivable.
Conclusions: Lemon peel Ecoenzyme (EE) is antimicrobial with effective biofilm-disrupting properties on a mature multi-species biofilm. However, its effects were slower than 3.5% sodium hypochlorite.</description><identifier>ISSN: 0972-0707</identifier><identifier>EISSN: 0974-5203</identifier><identifier>DOI: 10.4103/jcd.jcd_473_22</identifier><identifier>PMID: 37205894</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Bacterial infections ; Biofilms ; By-products ; Dentistry ; Disinfection ; Enzymes ; Fermentation ; Health aspects ; Methods ; Minimum inhibitory concentration ; Natural products ; Organic wastes ; Original ; Prevention ; Root canal therapy ; Secondary metabolites ; Sodium hypochlorite</subject><ispartof>Journal of conservative dentistry, 2023-03, Vol.26 (2), p.176-181</ispartof><rights>Copyright: © 2023 Journal of Conservative Dentistry.</rights><rights>COPYRIGHT 2023 Medknow Publications and Media Pvt. Ltd.</rights><rights>2023. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2023 Journal of Conservative Dentistry 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10190087/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10190087/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37205894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sai, Shamini</creatorcontrib><creatorcontrib>J. Abisha, V</creatorcontrib><creatorcontrib>Mahalakshmi, Krishnan</creatorcontrib><creatorcontrib>Veronica, Aruna</creatorcontrib><creatorcontrib>Susila, Anand</creatorcontrib><title>Treasure from trash - Is Ecoenzyme the new panacea in conservative dentistry and endodontics?</title><title>Journal of conservative dentistry</title><addtitle>J Conserv Dent</addtitle><description>Context: Endodontic biofilm eradication is achieved by chemo-mechanical disinfection. The search for a safer, nontoxic irrigant led us to a natural product, Ecoenzyme.
Aim: This study aims to analyze Ecoenzyme (EE) and explore its antimicrobial and biofilm disrupting activity against a 1-week mature multi-species biofilm.
Materials and Methods: Qualitative assessment of the phytochemicals present in EE was conducted. Minimal inhibitory concentration (MIC), minimum bactericidal concentration, and zone of inhibition (ZOI) were recorded. Multi-species biofilm of Streptococcus mutans (MTCC 497), Lactobacillus acidophilus (MTCC 10307), and Enterococcus faecalis (ATCC 29212) was grown and time-kill assay was performed to test biofilm disruption for EE, 3.5% sodium hypochlorite (NaOCl) (control). Student's t-test and one-way ANOVA with post hoc analysis were conducted for ZOI and time-kill assay, respectively. Statistical significance was set at P ≤ 0.05.
Results: EE contained secondary metabolites having antibacterial properties. MIC was 25% (S. mutans), 50% (E. faecalis), and >50% (L. acidophilus). EE disrupted ~90% of biofilm species in 5 min of exposure while NaOCl achieved ~99.9% reduction. Further reduction by EE progressed over 20 min after which no viable bacteria in the biofilm was cultivable.
Conclusions: Lemon peel Ecoenzyme (EE) is antimicrobial with effective biofilm-disrupting properties on a mature multi-species biofilm. However, its effects were slower than 3.5% sodium hypochlorite.</description><subject>Bacterial infections</subject><subject>Biofilms</subject><subject>By-products</subject><subject>Dentistry</subject><subject>Disinfection</subject><subject>Enzymes</subject><subject>Fermentation</subject><subject>Health aspects</subject><subject>Methods</subject><subject>Minimum inhibitory concentration</subject><subject>Natural products</subject><subject>Organic wastes</subject><subject>Original</subject><subject>Prevention</subject><subject>Root canal therapy</subject><subject>Secondary metabolites</subject><subject>Sodium hypochlorite</subject><issn>0972-0707</issn><issn>0974-5203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptksFvFCEUxidGY2v16tGQmBgvszLAwMypNk3VJk281KMhDDx22M7ACjO7Wf96WdvqrjGEQB6_98H7eEXxusILVmH6YaXNIk_JBJWEPClOcStYWRNMn_7ekxILLE6KFymtMOaMtfh5cUIFwXXTstPi-20EleYIyMYwoimq1KMSXSd0pQP4n7sR0NQD8rBFa-WVBoWcRzr4BHGjJrcBZMBPLk1xh5Q3CLwJJuSITucvi2dWDQlePaxnxbdPV7eXX8qbr5-vLy9uyiXlNZRcGdaB1hQ6bnFnlGmssoRqXZuOEN52lBNrcsG8bgAwoartRFs1lQVSU0vPivN73fXcjWB0flBUg1xHN6q4k0E5eXziXS-XYSMrXLUYNyIrvH9QiOHHDGmSo0sahkF5CHOSpKm44KLmNKNv_0FXYY4-1yeJaBlrsu0H1FINIJ23IV-s96LyQjCOMWWiytTiP1QeBkaXTQbrcvwo4d1BQg9qmPoUhnly-UeOwTeHlvzx4vHrM_DxHtiGYYKY7oZ5C1Fm9s6HbXZG7htM7pvrb4PJSnD52DL0F6M1xxc</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Sai, Shamini</creator><creator>J. Abisha, V</creator><creator>Mahalakshmi, Krishnan</creator><creator>Veronica, Aruna</creator><creator>Susila, Anand</creator><general>Wolters Kluwer India Pvt. Ltd</general><general>Medknow Publications and Media Pvt. Ltd</general><general>Medknow Publications & Media Pvt. Ltd</general><general>Wolters Kluwer - Medknow</general><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230301</creationdate><title>Treasure from trash - Is Ecoenzyme the new panacea in conservative dentistry and endodontics?</title><author>Sai, Shamini ; J. Abisha, V ; Mahalakshmi, Krishnan ; Veronica, Aruna ; Susila, Anand</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g365e-6ad4becc3eb6f0bdad8faf23cc5db2269b362fd410658ee023a9b79181fe253f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Bacterial infections</topic><topic>Biofilms</topic><topic>By-products</topic><topic>Dentistry</topic><topic>Disinfection</topic><topic>Enzymes</topic><topic>Fermentation</topic><topic>Health aspects</topic><topic>Methods</topic><topic>Minimum inhibitory concentration</topic><topic>Natural products</topic><topic>Organic wastes</topic><topic>Original</topic><topic>Prevention</topic><topic>Root canal therapy</topic><topic>Secondary metabolites</topic><topic>Sodium hypochlorite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sai, Shamini</creatorcontrib><creatorcontrib>J. Abisha, V</creatorcontrib><creatorcontrib>Mahalakshmi, Krishnan</creatorcontrib><creatorcontrib>Veronica, Aruna</creatorcontrib><creatorcontrib>Susila, Anand</creatorcontrib><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of conservative dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sai, Shamini</au><au>J. Abisha, V</au><au>Mahalakshmi, Krishnan</au><au>Veronica, Aruna</au><au>Susila, Anand</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treasure from trash - Is Ecoenzyme the new panacea in conservative dentistry and endodontics?</atitle><jtitle>Journal of conservative dentistry</jtitle><addtitle>J Conserv Dent</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>26</volume><issue>2</issue><spage>176</spage><epage>181</epage><pages>176-181</pages><issn>0972-0707</issn><eissn>0974-5203</eissn><abstract>Context: Endodontic biofilm eradication is achieved by chemo-mechanical disinfection. The search for a safer, nontoxic irrigant led us to a natural product, Ecoenzyme.
Aim: This study aims to analyze Ecoenzyme (EE) and explore its antimicrobial and biofilm disrupting activity against a 1-week mature multi-species biofilm.
Materials and Methods: Qualitative assessment of the phytochemicals present in EE was conducted. Minimal inhibitory concentration (MIC), minimum bactericidal concentration, and zone of inhibition (ZOI) were recorded. Multi-species biofilm of Streptococcus mutans (MTCC 497), Lactobacillus acidophilus (MTCC 10307), and Enterococcus faecalis (ATCC 29212) was grown and time-kill assay was performed to test biofilm disruption for EE, 3.5% sodium hypochlorite (NaOCl) (control). Student's t-test and one-way ANOVA with post hoc analysis were conducted for ZOI and time-kill assay, respectively. Statistical significance was set at P ≤ 0.05.
Results: EE contained secondary metabolites having antibacterial properties. MIC was 25% (S. mutans), 50% (E. faecalis), and >50% (L. acidophilus). EE disrupted ~90% of biofilm species in 5 min of exposure while NaOCl achieved ~99.9% reduction. Further reduction by EE progressed over 20 min after which no viable bacteria in the biofilm was cultivable.
Conclusions: Lemon peel Ecoenzyme (EE) is antimicrobial with effective biofilm-disrupting properties on a mature multi-species biofilm. However, its effects were slower than 3.5% sodium hypochlorite.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>37205894</pmid><doi>10.4103/jcd.jcd_473_22</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0972-0707 |
| ispartof | Journal of conservative dentistry, 2023-03, Vol.26 (2), p.176-181 |
| issn | 0972-0707 0974-5203 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10190087 |
| source | PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bacterial infections Biofilms By-products Dentistry Disinfection Enzymes Fermentation Health aspects Methods Minimum inhibitory concentration Natural products Organic wastes Original Prevention Root canal therapy Secondary metabolites Sodium hypochlorite |
| title | Treasure from trash - Is Ecoenzyme the new panacea in conservative dentistry and endodontics? |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T07%3A57%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Treasure%20from%20trash%20-%20Is%20Ecoenzyme%20the%20new%20panacea%20in%20conservative%20dentistry%20and%20endodontics?&rft.jtitle=Journal%20of%20conservative%20dentistry&rft.au=Sai,%20Shamini&rft.date=2023-03-01&rft.volume=26&rft.issue=2&rft.spage=176&rft.epage=181&rft.pages=176-181&rft.issn=0972-0707&rft.eissn=0974-5203&rft_id=info:doi/10.4103/jcd.jcd_473_22&rft_dat=%3Cgale_pubme%3EA746003471%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2794480973&rft_id=info:pmid/37205894&rft_galeid=A746003471&rfr_iscdi=true |