DNA/RNA helicase DHX36 is required for late stages of spermatogenesis
ABSTRACT Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly...
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| Veröffentlicht in: | Journal of molecular cell biology 2023-04, Vol.14 (11) |
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| container_title | Journal of molecular cell biology |
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| creator | Zhang, Kejia Zhang, Tianxin Zhang, Yujie Yuan, Jinyu Tang, Xinzhe Zhang, Chaobao Yin, Qianqian Zhang, Yonglian Tong, Ming-Han |
| description | ABSTRACT
Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis. |
| doi_str_mv | 10.1093/jmcb/mjac069 |
| format | Article |
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Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis.</description><identifier>ISSN: 1674-2788</identifier><identifier>EISSN: 1759-4685</identifier><identifier>DOI: 10.1093/jmcb/mjac069</identifier><identifier>PMID: 36484653</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Animals ; DNA - genetics ; DNA Helicases - genetics ; Male ; Meiosis ; Mice ; RNA ; RNA Helicases - genetics ; Spermatocytes ; Spermatogenesis - genetics</subject><ispartof>Journal of molecular cell biology, 2023-04, Vol.14 (11)</ispartof><rights>The Author(s) (2022). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology , CEMCS, CAS. 2022</rights><rights>The Author(s) (2022). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, CEMCS, CAS.</rights><rights>The Author(s) (2022). Published by Oxford University Press on behalf of , CEMCS, CAS. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b9d0d8006faddf02cb1e067642beebe800657c9c1193919a5cb717f1e8f09b9b3</citedby><cites>FETCH-LOGICAL-c417t-b9d0d8006faddf02cb1e067642beebe800657c9c1193919a5cb717f1e8f09b9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166722/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166722/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1604,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36484653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yao, Xuebiao</contributor><creatorcontrib>Zhang, Kejia</creatorcontrib><creatorcontrib>Zhang, Tianxin</creatorcontrib><creatorcontrib>Zhang, Yujie</creatorcontrib><creatorcontrib>Yuan, Jinyu</creatorcontrib><creatorcontrib>Tang, Xinzhe</creatorcontrib><creatorcontrib>Zhang, Chaobao</creatorcontrib><creatorcontrib>Yin, Qianqian</creatorcontrib><creatorcontrib>Zhang, Yonglian</creatorcontrib><creatorcontrib>Tong, Ming-Han</creatorcontrib><title>DNA/RNA helicase DHX36 is required for late stages of spermatogenesis</title><title>Journal of molecular cell biology</title><addtitle>J Mol Cell Biol</addtitle><description>ABSTRACT
Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis.</description><subject>Animals</subject><subject>DNA - genetics</subject><subject>DNA Helicases - genetics</subject><subject>Male</subject><subject>Meiosis</subject><subject>Mice</subject><subject>RNA</subject><subject>RNA Helicases - genetics</subject><subject>Spermatocytes</subject><subject>Spermatogenesis - genetics</subject><issn>1674-2788</issn><issn>1759-4685</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><sourceid>EIF</sourceid><recordid>eNp9kMFLwzAUh4MoTuZuniU3PViXNG3SnGRs0wljgih4C0n6OjvaZSat4H9vx-bQi-_yHryP33t8CF1QckuJZMNVbc2wXmlLuDxCZ1SkMkp4lh53MxdJFIss66FBCCvSFcsYy8gp6jGeZAlP2RmaThaj4fNihN-hKq0OgCezN8ZxGbCHj7b0kOPCeVzpBnBo9BICdgUOG_C1btwS1hDKcI5OCl0FGOx7H73eT1_Gs2j-9PA4Hs0jm1DRREbmJM8I4YXO84LE1lAgXPAkNgAGtptUWGkplUxSqVNrBBUFhawg0kjD-uhul7tpTQ25hXXjdaU2vqy1_1JOl-rvZl2-q6X7VJRQzkUcdwnX-wTvPloIjarLYKGq9BpcG1QsUhZLwRLRoTc71HoXgoficIcStbWvtvbV3n6HX_7-7QD_uO6Aqx3g2s3_Ud8Eio7h</recordid><startdate>20230406</startdate><enddate>20230406</enddate><creator>Zhang, Kejia</creator><creator>Zhang, Tianxin</creator><creator>Zhang, Yujie</creator><creator>Yuan, Jinyu</creator><creator>Tang, Xinzhe</creator><creator>Zhang, Chaobao</creator><creator>Yin, Qianqian</creator><creator>Zhang, Yonglian</creator><creator>Tong, Ming-Han</creator><general>Oxford University Press</general><scope>TOX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230406</creationdate><title>DNA/RNA helicase DHX36 is required for late stages of spermatogenesis</title><author>Zhang, Kejia ; Zhang, Tianxin ; Zhang, Yujie ; Yuan, Jinyu ; Tang, Xinzhe ; Zhang, Chaobao ; Yin, Qianqian ; Zhang, Yonglian ; Tong, Ming-Han</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b9d0d8006faddf02cb1e067642beebe800657c9c1193919a5cb717f1e8f09b9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>DNA - genetics</topic><topic>DNA Helicases - genetics</topic><topic>Male</topic><topic>Meiosis</topic><topic>Mice</topic><topic>RNA</topic><topic>RNA Helicases - genetics</topic><topic>Spermatocytes</topic><topic>Spermatogenesis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Kejia</creatorcontrib><creatorcontrib>Zhang, Tianxin</creatorcontrib><creatorcontrib>Zhang, Yujie</creatorcontrib><creatorcontrib>Yuan, Jinyu</creatorcontrib><creatorcontrib>Tang, Xinzhe</creatorcontrib><creatorcontrib>Zhang, Chaobao</creatorcontrib><creatorcontrib>Yin, Qianqian</creatorcontrib><creatorcontrib>Zhang, Yonglian</creatorcontrib><creatorcontrib>Tong, Ming-Han</creatorcontrib><collection>Oxford Journals Open Access Collection</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of molecular cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Kejia</au><au>Zhang, Tianxin</au><au>Zhang, Yujie</au><au>Yuan, Jinyu</au><au>Tang, Xinzhe</au><au>Zhang, Chaobao</au><au>Yin, Qianqian</au><au>Zhang, Yonglian</au><au>Tong, Ming-Han</au><au>Yao, Xuebiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA/RNA helicase DHX36 is required for late stages of spermatogenesis</atitle><jtitle>Journal of molecular cell biology</jtitle><addtitle>J Mol Cell Biol</addtitle><date>2023-04-06</date><risdate>2023</risdate><volume>14</volume><issue>11</issue><issn>1674-2788</issn><eissn>1759-4685</eissn><abstract>ABSTRACT
Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>36484653</pmid><doi>10.1093/jmcb/mjac069</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals DNA - genetics DNA Helicases - genetics Male Meiosis Mice RNA RNA Helicases - genetics Spermatocytes Spermatogenesis - genetics |
| title | DNA/RNA helicase DHX36 is required for late stages of spermatogenesis |
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