Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production
The type three secretion system (T3SS) is a major virulence system of ( ). The effector protein Exotoxin S (ExoS) produced by is secreted into the host cells via the T3SS. For the purpose of an experiment on inhibitors with regard to ExoS secretion, we developed a sandwich-type enzyme-linked immunos...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2023-04, Vol.33 (4), p.430-440 |
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| creator | Ahn, Hye In Jang, Hyun-Jae Kwon, Ok-Kyoung Kim, Jung-Hee Oh, Jae-Hoon Kim, Seung-Ho Oh, Sei-Ryang Han, Sang-Bae Ahn, Kyung-Seop Park, Ji-Won |
| description | The type three secretion system (T3SS) is a major virulence system of
(
). The effector protein Exotoxin S (ExoS) produced by
is secreted into the host cells via the T3SS. For the purpose of an experiment on inhibitors with regard to ExoS secretion, we developed a sandwich-type enzyme-linked immunosorbent assay (ELISA) system. Quercetin was selected because it has a prominent ExoS inhibition effect and also is known to have anti-inflammatory and antioxidant effects on mammalian cells. In this study, we investigated the effects of quercetin on the expression and secretion of ExoS using ELISA and Western blot analysis methods. The results showed that the secretion of ExoS was significantly decreased by 10 and 20 μM of quercetin. Also,
,
,
, and
which are composed of the T3SS needle, are reduced by quercetin at the mRNA level. We also confirmed the inhibitory effect of quercetin on cytokines (IL-6, IL-1β, and IL-18) in
-infected H292 cells by real-time polymerase chain reaction (PCR) and ELISA. Collectively, quercetin inhibits the secretion of ExoS by reducing both ExoS production and the expression of the needle protein of T3SS. Furthermore, these results suggest that quercetin has the potential to be used as an anti-toxic treatment for the inflammatory disease caused by
infection. |
| doi_str_mv | 10.4014/jmb.2208.08034 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10164726</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>36788468</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-e620b4a4a6efce13ee955322090b15d6425a8ec5525366658eff7a11e7d171023</originalsourceid><addsrcrecordid>eNpVkdtOGzEQhq0KVCjtbS-RX2BTH9aHXCEUQRMpFaC215Z3dzYYdu3I9kLzRjwmTmkRXM2MZv5vRvMj9JWSWU1o_e1ubGaMET0jmvD6AzqmiutKa8UOSk6oqpRm4gh9SumOEEmZlh_REZdK61rqY_R0M0FsITuPz3MGP9kMCedbwNcxdFObXfA49PuqWvl-sONoc4g7vNjlcO98GS7SJZszvJxG6_F68ht8sXUFMTg74AUMQ8JFCm2GDj-WBr5OMHVhDN4mbCFOG-dDsrjZ4R9l52DLNYXxJ_x8c8RndNjbIcGXf_EE_b68-LVYVuur76vF-bpq-ZzmCiQjTW1rK6FvgXKAuRC8fGhOGio6WTNhNbRCMMGllEJD3ytLKaiOKkoYP0FnL9zt1IzQteBztIPZRjfauDPBOvO-492t2YQHU54ta8VkIcxeCG0MKUXoX8WUmL1ppphm9qaZv6YVwenbla_j_13iz1NBl0Y</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Ahn, Hye In ; Jang, Hyun-Jae ; Kwon, Ok-Kyoung ; Kim, Jung-Hee ; Oh, Jae-Hoon ; Kim, Seung-Ho ; Oh, Sei-Ryang ; Han, Sang-Bae ; Ahn, Kyung-Seop ; Park, Ji-Won</creator><creatorcontrib>Ahn, Hye In ; Jang, Hyun-Jae ; Kwon, Ok-Kyoung ; Kim, Jung-Hee ; Oh, Jae-Hoon ; Kim, Seung-Ho ; Oh, Sei-Ryang ; Han, Sang-Bae ; Ahn, Kyung-Seop ; Park, Ji-Won</creatorcontrib><description>The type three secretion system (T3SS) is a major virulence system of
(
). The effector protein Exotoxin S (ExoS) produced by
is secreted into the host cells via the T3SS. For the purpose of an experiment on inhibitors with regard to ExoS secretion, we developed a sandwich-type enzyme-linked immunosorbent assay (ELISA) system. Quercetin was selected because it has a prominent ExoS inhibition effect and also is known to have anti-inflammatory and antioxidant effects on mammalian cells. In this study, we investigated the effects of quercetin on the expression and secretion of ExoS using ELISA and Western blot analysis methods. The results showed that the secretion of ExoS was significantly decreased by 10 and 20 μM of quercetin. Also,
,
,
, and
which are composed of the T3SS needle, are reduced by quercetin at the mRNA level. We also confirmed the inhibitory effect of quercetin on cytokines (IL-6, IL-1β, and IL-18) in
-infected H292 cells by real-time polymerase chain reaction (PCR) and ELISA. Collectively, quercetin inhibits the secretion of ExoS by reducing both ExoS production and the expression of the needle protein of T3SS. Furthermore, these results suggest that quercetin has the potential to be used as an anti-toxic treatment for the inflammatory disease caused by
infection.</description><identifier>ISSN: 1017-7825</identifier><identifier>EISSN: 1738-8872</identifier><identifier>DOI: 10.4014/jmb.2208.08034</identifier><identifier>PMID: 36788468</identifier><language>eng</language><publisher>Korea (South): The Korean Society for Microbiology and Biotechnology</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - genetics ; Cytokines - metabolism ; Epithelial Cells - metabolism ; Exotoxins ; Humans ; Lung - metabolism ; Mammals - metabolism ; Pseudomonas aeruginosa - genetics ; Pseudomonas Infections - drug therapy ; Quercetin - metabolism ; Quercetin - pharmacology ; Research article</subject><ispartof>Journal of microbiology and biotechnology, 2023-04, Vol.33 (4), p.430-440</ispartof><rights>Copyright © 2023 by the authors. Licensee KMB 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-e620b4a4a6efce13ee955322090b15d6425a8ec5525366658eff7a11e7d171023</citedby><cites>FETCH-LOGICAL-c391t-e620b4a4a6efce13ee955322090b15d6425a8ec5525366658eff7a11e7d171023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164726/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164726/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36788468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahn, Hye In</creatorcontrib><creatorcontrib>Jang, Hyun-Jae</creatorcontrib><creatorcontrib>Kwon, Ok-Kyoung</creatorcontrib><creatorcontrib>Kim, Jung-Hee</creatorcontrib><creatorcontrib>Oh, Jae-Hoon</creatorcontrib><creatorcontrib>Kim, Seung-Ho</creatorcontrib><creatorcontrib>Oh, Sei-Ryang</creatorcontrib><creatorcontrib>Han, Sang-Bae</creatorcontrib><creatorcontrib>Ahn, Kyung-Seop</creatorcontrib><creatorcontrib>Park, Ji-Won</creatorcontrib><title>Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production</title><title>Journal of microbiology and biotechnology</title><addtitle>J Microbiol Biotechnol</addtitle><description>The type three secretion system (T3SS) is a major virulence system of
(
). The effector protein Exotoxin S (ExoS) produced by
is secreted into the host cells via the T3SS. For the purpose of an experiment on inhibitors with regard to ExoS secretion, we developed a sandwich-type enzyme-linked immunosorbent assay (ELISA) system. Quercetin was selected because it has a prominent ExoS inhibition effect and also is known to have anti-inflammatory and antioxidant effects on mammalian cells. In this study, we investigated the effects of quercetin on the expression and secretion of ExoS using ELISA and Western blot analysis methods. The results showed that the secretion of ExoS was significantly decreased by 10 and 20 μM of quercetin. Also,
,
,
, and
which are composed of the T3SS needle, are reduced by quercetin at the mRNA level. We also confirmed the inhibitory effect of quercetin on cytokines (IL-6, IL-1β, and IL-18) in
-infected H292 cells by real-time polymerase chain reaction (PCR) and ELISA. Collectively, quercetin inhibits the secretion of ExoS by reducing both ExoS production and the expression of the needle protein of T3SS. Furthermore, these results suggest that quercetin has the potential to be used as an anti-toxic treatment for the inflammatory disease caused by
infection.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - genetics</subject><subject>Cytokines - metabolism</subject><subject>Epithelial Cells - metabolism</subject><subject>Exotoxins</subject><subject>Humans</subject><subject>Lung - metabolism</subject><subject>Mammals - metabolism</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas Infections - drug therapy</subject><subject>Quercetin - metabolism</subject><subject>Quercetin - pharmacology</subject><subject>Research article</subject><issn>1017-7825</issn><issn>1738-8872</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkdtOGzEQhq0KVCjtbS-RX2BTH9aHXCEUQRMpFaC215Z3dzYYdu3I9kLzRjwmTmkRXM2MZv5vRvMj9JWSWU1o_e1ubGaMET0jmvD6AzqmiutKa8UOSk6oqpRm4gh9SumOEEmZlh_REZdK61rqY_R0M0FsITuPz3MGP9kMCedbwNcxdFObXfA49PuqWvl-sONoc4g7vNjlcO98GS7SJZszvJxG6_F68ht8sXUFMTg74AUMQ8JFCm2GDj-WBr5OMHVhDN4mbCFOG-dDsrjZ4R9l52DLNYXxJ_x8c8RndNjbIcGXf_EE_b68-LVYVuur76vF-bpq-ZzmCiQjTW1rK6FvgXKAuRC8fGhOGio6WTNhNbRCMMGllEJD3ytLKaiOKkoYP0FnL9zt1IzQteBztIPZRjfauDPBOvO-492t2YQHU54ta8VkIcxeCG0MKUXoX8WUmL1ppphm9qaZv6YVwenbla_j_13iz1NBl0Y</recordid><startdate>20230428</startdate><enddate>20230428</enddate><creator>Ahn, Hye In</creator><creator>Jang, Hyun-Jae</creator><creator>Kwon, Ok-Kyoung</creator><creator>Kim, Jung-Hee</creator><creator>Oh, Jae-Hoon</creator><creator>Kim, Seung-Ho</creator><creator>Oh, Sei-Ryang</creator><creator>Han, Sang-Bae</creator><creator>Ahn, Kyung-Seop</creator><creator>Park, Ji-Won</creator><general>The Korean Society for Microbiology and Biotechnology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20230428</creationdate><title>Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production</title><author>Ahn, Hye In ; Jang, Hyun-Jae ; Kwon, Ok-Kyoung ; Kim, Jung-Hee ; Oh, Jae-Hoon ; Kim, Seung-Ho ; Oh, Sei-Ryang ; Han, Sang-Bae ; Ahn, Kyung-Seop ; Park, Ji-Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-e620b4a4a6efce13ee955322090b15d6425a8ec5525366658eff7a11e7d171023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Toxins - genetics</topic><topic>Cytokines - metabolism</topic><topic>Epithelial Cells - metabolism</topic><topic>Exotoxins</topic><topic>Humans</topic><topic>Lung - metabolism</topic><topic>Mammals - metabolism</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas Infections - drug therapy</topic><topic>Quercetin - metabolism</topic><topic>Quercetin - pharmacology</topic><topic>Research article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahn, Hye In</creatorcontrib><creatorcontrib>Jang, Hyun-Jae</creatorcontrib><creatorcontrib>Kwon, Ok-Kyoung</creatorcontrib><creatorcontrib>Kim, Jung-Hee</creatorcontrib><creatorcontrib>Oh, Jae-Hoon</creatorcontrib><creatorcontrib>Kim, Seung-Ho</creatorcontrib><creatorcontrib>Oh, Sei-Ryang</creatorcontrib><creatorcontrib>Han, Sang-Bae</creatorcontrib><creatorcontrib>Ahn, Kyung-Seop</creatorcontrib><creatorcontrib>Park, Ji-Won</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahn, Hye In</au><au>Jang, Hyun-Jae</au><au>Kwon, Ok-Kyoung</au><au>Kim, Jung-Hee</au><au>Oh, Jae-Hoon</au><au>Kim, Seung-Ho</au><au>Oh, Sei-Ryang</au><au>Han, Sang-Bae</au><au>Ahn, Kyung-Seop</au><au>Park, Ji-Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><addtitle>J Microbiol Biotechnol</addtitle><date>2023-04-28</date><risdate>2023</risdate><volume>33</volume><issue>4</issue><spage>430</spage><epage>440</epage><pages>430-440</pages><issn>1017-7825</issn><eissn>1738-8872</eissn><abstract>The type three secretion system (T3SS) is a major virulence system of
(
). The effector protein Exotoxin S (ExoS) produced by
is secreted into the host cells via the T3SS. For the purpose of an experiment on inhibitors with regard to ExoS secretion, we developed a sandwich-type enzyme-linked immunosorbent assay (ELISA) system. Quercetin was selected because it has a prominent ExoS inhibition effect and also is known to have anti-inflammatory and antioxidant effects on mammalian cells. In this study, we investigated the effects of quercetin on the expression and secretion of ExoS using ELISA and Western blot analysis methods. The results showed that the secretion of ExoS was significantly decreased by 10 and 20 μM of quercetin. Also,
,
,
, and
which are composed of the T3SS needle, are reduced by quercetin at the mRNA level. We also confirmed the inhibitory effect of quercetin on cytokines (IL-6, IL-1β, and IL-18) in
-infected H292 cells by real-time polymerase chain reaction (PCR) and ELISA. Collectively, quercetin inhibits the secretion of ExoS by reducing both ExoS production and the expression of the needle protein of T3SS. Furthermore, these results suggest that quercetin has the potential to be used as an anti-toxic treatment for the inflammatory disease caused by
infection.</abstract><cop>Korea (South)</cop><pub>The Korean Society for Microbiology and Biotechnology</pub><pmid>36788468</pmid><doi>10.4014/jmb.2208.08034</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1017-7825 1738-8872 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - genetics Cytokines - metabolism Epithelial Cells - metabolism Exotoxins Humans Lung - metabolism Mammals - metabolism Pseudomonas aeruginosa - genetics Pseudomonas Infections - drug therapy Quercetin - metabolism Quercetin - pharmacology Research article |
| title | Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production |
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