Role of Cryptosporidium spp in Development of Colorectal Cancer
Colorectal cancer is one of the most common malignancies in humans. About 20% of the cancer incidence was attributed to infectious agents highlighting the association between infectious agents and the development of cancers. It has been suspected that Cryptosporidium spp. infection may be correlated...
Gespeichert in:
| Veröffentlicht in: | Asian Pacific Journal of Cancer Prevention 2023-02, Vol.24 (2), p.667-674 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 674 |
|---|---|
| container_issue | 2 |
| container_start_page | 667 |
| container_title | Asian Pacific Journal of Cancer Prevention |
| container_volume | 24 |
| creator | Abd El-Latif, Naglaa Fathi Kandil, Noha Said Shamseya, Mohamed Elwany, Yasmine Nagy Ibrahim, Heba Said |
| description | Colorectal cancer is one of the most common malignancies in humans. About 20% of the cancer incidence was attributed to infectious agents highlighting the association between infectious agents and the development of cancers. It has been suspected that Cryptosporidium spp. infection may be correlated with colon adenocarcinoma. Aim: investigate the percentage of cryptosporidiosis among colorectal cancer patients.
100 patients were recruited from Medical Research Institute, Alexandria University.
Fresh stool specimens were collected, homogenized and examined using direct wet mount and by permanent staining of faecal smears using Modified ZN staining. Molecular detection by PCR amplification of Cryptosporidium COWP gene.
Significantly higher proportion of colorectal cancer patients (32.5%, 42.5%) tested positive by MZN and ELISA respectively compared to only 3.3% and 5% of positive MZN and ELISA among control group. Also, positive PCR was detected among higher proportion of colorectal cancer patients (47.5%) and only 5% of control group. Odds of colorectal cancer is 19 times among positive cases of Cryptosporidium by PCR than those without proven infection by PCR (OR 19.12; 95% CI 4.82-75.99). Comparison of the assessment of Cryptosporidium infection made by two techniques produces a kappa value of 0.770, and .759 respectively between NZN, ELISA and PCR as a gold standard, suggesting a good agreement between the two techniques and PCR. This value of kappa is significantly different from zero, K.770, p |
| doi_str_mv | 10.31557/APJCP.2023.24.2.667 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10162599</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>36853318</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3247-78f6ddb52763c26d248d597e4a128aec7d41dae2b6b3ee6dbc7e23c4d3aaf7ba3</originalsourceid><addsrcrecordid>eNpVkN1Kw0AQhRdRbK2-gUheoDE7-5delRL_KVhEwbtlszvRSJINm7bQtze2WurVDMw5Zw4fIZc0iRkVQl3PFk_ZIoYEWAw8hlhKdUSGwJUcKwnvxwf7gJx13VeScJEqcUoGTKaCMZoOyfTFVxj5IsrCpl36rvWhdOWqjrq2jcomusE1Vr6tsVluVb7yAe3SVFFmGovhnJwUpurw4neOyNvd7Wv2MJ4_3z9ms_nYsr7FWKWFdC4XoCSzIB3w1ImJQm4opAatcpw6g5DLnCFKl1uFwCx3zJhC5YaNyHSX267yGp3t-wRT6TaUtQkb7U2p_1-a8lN_-LWmCZUgJpM-ge8SbPBdF7DYm2mit0T1lqj-IaqBa9A90d52dfh4b_pDyL4BbeN0zQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Role of Cryptosporidium spp in Development of Colorectal Cancer</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free E- Journals</source><creator>Abd El-Latif, Naglaa Fathi ; Kandil, Noha Said ; Shamseya, Mohamed ; Elwany, Yasmine Nagy ; Ibrahim, Heba Said</creator><creatorcontrib>Abd El-Latif, Naglaa Fathi ; Kandil, Noha Said ; Shamseya, Mohamed ; Elwany, Yasmine Nagy ; Ibrahim, Heba Said</creatorcontrib><description>Colorectal cancer is one of the most common malignancies in humans. About 20% of the cancer incidence was attributed to infectious agents highlighting the association between infectious agents and the development of cancers. It has been suspected that Cryptosporidium spp. infection may be correlated with colon adenocarcinoma. Aim: investigate the percentage of cryptosporidiosis among colorectal cancer patients.
100 patients were recruited from Medical Research Institute, Alexandria University.
Fresh stool specimens were collected, homogenized and examined using direct wet mount and by permanent staining of faecal smears using Modified ZN staining. Molecular detection by PCR amplification of Cryptosporidium COWP gene.
Significantly higher proportion of colorectal cancer patients (32.5%, 42.5%) tested positive by MZN and ELISA respectively compared to only 3.3% and 5% of positive MZN and ELISA among control group. Also, positive PCR was detected among higher proportion of colorectal cancer patients (47.5%) and only 5% of control group. Odds of colorectal cancer is 19 times among positive cases of Cryptosporidium by PCR than those without proven infection by PCR (OR 19.12; 95% CI 4.82-75.99). Comparison of the assessment of Cryptosporidium infection made by two techniques produces a kappa value of 0.770, and .759 respectively between NZN, ELISA and PCR as a gold standard, suggesting a good agreement between the two techniques and PCR. This value of kappa is significantly different from zero, K.770, p<0.001 for MZN and K.759, p<.001 for ELISA. Specificity of MZN (100%) is higher than that of ELISA (96.2%) and both reported higher specificity than sensitivity denoting that both tests are good positive to rule in the presence of infection at 40% prevalence.
Cryptosporidium infection is significantly higher among cancer colon patients reinforcing that it might be considered as a likely risk factor for the development cancer colon.</description><identifier>ISSN: 2476-762X</identifier><identifier>ISSN: 1513-7368</identifier><identifier>EISSN: 2476-762X</identifier><identifier>DOI: 10.31557/APJCP.2023.24.2.667</identifier><identifier>PMID: 36853318</identifier><language>eng</language><publisher>Thailand: West Asia Organization for Cancer Prevention</publisher><subject>Adenocarcinoma ; Colonic Neoplasms ; Cryptosporidiosis - complications ; Cryptosporidiosis - epidemiology ; Cryptosporidium - genetics ; Humans ; Polymerase Chain Reaction</subject><ispartof>Asian Pacific Journal of Cancer Prevention, 2023-02, Vol.24 (2), p.667-674</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3247-78f6ddb52763c26d248d597e4a128aec7d41dae2b6b3ee6dbc7e23c4d3aaf7ba3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162599/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162599/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36853318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abd El-Latif, Naglaa Fathi</creatorcontrib><creatorcontrib>Kandil, Noha Said</creatorcontrib><creatorcontrib>Shamseya, Mohamed</creatorcontrib><creatorcontrib>Elwany, Yasmine Nagy</creatorcontrib><creatorcontrib>Ibrahim, Heba Said</creatorcontrib><title>Role of Cryptosporidium spp in Development of Colorectal Cancer</title><title>Asian Pacific Journal of Cancer Prevention</title><addtitle>Asian Pac J Cancer Prev</addtitle><description>Colorectal cancer is one of the most common malignancies in humans. About 20% of the cancer incidence was attributed to infectious agents highlighting the association between infectious agents and the development of cancers. It has been suspected that Cryptosporidium spp. infection may be correlated with colon adenocarcinoma. Aim: investigate the percentage of cryptosporidiosis among colorectal cancer patients.
100 patients were recruited from Medical Research Institute, Alexandria University.
Fresh stool specimens were collected, homogenized and examined using direct wet mount and by permanent staining of faecal smears using Modified ZN staining. Molecular detection by PCR amplification of Cryptosporidium COWP gene.
Significantly higher proportion of colorectal cancer patients (32.5%, 42.5%) tested positive by MZN and ELISA respectively compared to only 3.3% and 5% of positive MZN and ELISA among control group. Also, positive PCR was detected among higher proportion of colorectal cancer patients (47.5%) and only 5% of control group. Odds of colorectal cancer is 19 times among positive cases of Cryptosporidium by PCR than those without proven infection by PCR (OR 19.12; 95% CI 4.82-75.99). Comparison of the assessment of Cryptosporidium infection made by two techniques produces a kappa value of 0.770, and .759 respectively between NZN, ELISA and PCR as a gold standard, suggesting a good agreement between the two techniques and PCR. This value of kappa is significantly different from zero, K.770, p<0.001 for MZN and K.759, p<.001 for ELISA. Specificity of MZN (100%) is higher than that of ELISA (96.2%) and both reported higher specificity than sensitivity denoting that both tests are good positive to rule in the presence of infection at 40% prevalence.
Cryptosporidium infection is significantly higher among cancer colon patients reinforcing that it might be considered as a likely risk factor for the development cancer colon.</description><subject>Adenocarcinoma</subject><subject>Colonic Neoplasms</subject><subject>Cryptosporidiosis - complications</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidium - genetics</subject><subject>Humans</subject><subject>Polymerase Chain Reaction</subject><issn>2476-762X</issn><issn>1513-7368</issn><issn>2476-762X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkN1Kw0AQhRdRbK2-gUheoDE7-5delRL_KVhEwbtlszvRSJINm7bQtze2WurVDMw5Zw4fIZc0iRkVQl3PFk_ZIoYEWAw8hlhKdUSGwJUcKwnvxwf7gJx13VeScJEqcUoGTKaCMZoOyfTFVxj5IsrCpl36rvWhdOWqjrq2jcomusE1Vr6tsVluVb7yAe3SVFFmGovhnJwUpurw4neOyNvd7Wv2MJ4_3z9ms_nYsr7FWKWFdC4XoCSzIB3w1ImJQm4opAatcpw6g5DLnCFKl1uFwCx3zJhC5YaNyHSX267yGp3t-wRT6TaUtQkb7U2p_1-a8lN_-LWmCZUgJpM-ge8SbPBdF7DYm2mit0T1lqj-IaqBa9A90d52dfh4b_pDyL4BbeN0zQ</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Abd El-Latif, Naglaa Fathi</creator><creator>Kandil, Noha Said</creator><creator>Shamseya, Mohamed</creator><creator>Elwany, Yasmine Nagy</creator><creator>Ibrahim, Heba Said</creator><general>West Asia Organization for Cancer Prevention</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20230201</creationdate><title>Role of Cryptosporidium spp in Development of Colorectal Cancer</title><author>Abd El-Latif, Naglaa Fathi ; Kandil, Noha Said ; Shamseya, Mohamed ; Elwany, Yasmine Nagy ; Ibrahim, Heba Said</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3247-78f6ddb52763c26d248d597e4a128aec7d41dae2b6b3ee6dbc7e23c4d3aaf7ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adenocarcinoma</topic><topic>Colonic Neoplasms</topic><topic>Cryptosporidiosis - complications</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidium - genetics</topic><topic>Humans</topic><topic>Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abd El-Latif, Naglaa Fathi</creatorcontrib><creatorcontrib>Kandil, Noha Said</creatorcontrib><creatorcontrib>Shamseya, Mohamed</creatorcontrib><creatorcontrib>Elwany, Yasmine Nagy</creatorcontrib><creatorcontrib>Ibrahim, Heba Said</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Asian Pacific Journal of Cancer Prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abd El-Latif, Naglaa Fathi</au><au>Kandil, Noha Said</au><au>Shamseya, Mohamed</au><au>Elwany, Yasmine Nagy</au><au>Ibrahim, Heba Said</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Cryptosporidium spp in Development of Colorectal Cancer</atitle><jtitle>Asian Pacific Journal of Cancer Prevention</jtitle><addtitle>Asian Pac J Cancer Prev</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>24</volume><issue>2</issue><spage>667</spage><epage>674</epage><pages>667-674</pages><issn>2476-762X</issn><issn>1513-7368</issn><eissn>2476-762X</eissn><abstract>Colorectal cancer is one of the most common malignancies in humans. About 20% of the cancer incidence was attributed to infectious agents highlighting the association between infectious agents and the development of cancers. It has been suspected that Cryptosporidium spp. infection may be correlated with colon adenocarcinoma. Aim: investigate the percentage of cryptosporidiosis among colorectal cancer patients.
100 patients were recruited from Medical Research Institute, Alexandria University.
Fresh stool specimens were collected, homogenized and examined using direct wet mount and by permanent staining of faecal smears using Modified ZN staining. Molecular detection by PCR amplification of Cryptosporidium COWP gene.
Significantly higher proportion of colorectal cancer patients (32.5%, 42.5%) tested positive by MZN and ELISA respectively compared to only 3.3% and 5% of positive MZN and ELISA among control group. Also, positive PCR was detected among higher proportion of colorectal cancer patients (47.5%) and only 5% of control group. Odds of colorectal cancer is 19 times among positive cases of Cryptosporidium by PCR than those without proven infection by PCR (OR 19.12; 95% CI 4.82-75.99). Comparison of the assessment of Cryptosporidium infection made by two techniques produces a kappa value of 0.770, and .759 respectively between NZN, ELISA and PCR as a gold standard, suggesting a good agreement between the two techniques and PCR. This value of kappa is significantly different from zero, K.770, p<0.001 for MZN and K.759, p<.001 for ELISA. Specificity of MZN (100%) is higher than that of ELISA (96.2%) and both reported higher specificity than sensitivity denoting that both tests are good positive to rule in the presence of infection at 40% prevalence.
Cryptosporidium infection is significantly higher among cancer colon patients reinforcing that it might be considered as a likely risk factor for the development cancer colon.</abstract><cop>Thailand</cop><pub>West Asia Organization for Cancer Prevention</pub><pmid>36853318</pmid><doi>10.31557/APJCP.2023.24.2.667</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2476-762X |
| ispartof | Asian Pacific Journal of Cancer Prevention, 2023-02, Vol.24 (2), p.667-674 |
| issn | 2476-762X 1513-7368 2476-762X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10162599 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free E- Journals |
| subjects | Adenocarcinoma Colonic Neoplasms Cryptosporidiosis - complications Cryptosporidiosis - epidemiology Cryptosporidium - genetics Humans Polymerase Chain Reaction |
| title | Role of Cryptosporidium spp in Development of Colorectal Cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T23%3A15%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20Cryptosporidium%20spp%20in%20Development%20of%20Colorectal%20Cancer&rft.jtitle=Asian%20Pacific%20Journal%20of%20Cancer%20Prevention&rft.au=Abd%20El-Latif,%20Naglaa%20Fathi&rft.date=2023-02-01&rft.volume=24&rft.issue=2&rft.spage=667&rft.epage=674&rft.pages=667-674&rft.issn=2476-762X&rft.eissn=2476-762X&rft_id=info:doi/10.31557/APJCP.2023.24.2.667&rft_dat=%3Cpubmed_cross%3E36853318%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/36853318&rfr_iscdi=true |