Effect of ascorbic acid and epidermal growth factor in a rat tibia defect
Bone repair aims to restore the anatomical, biomechanical, and functional integrity of the affected structure. Here we study the effects of ascorbic acid (AA) and epidermal growth factor (EGF) applied in a single dose and in combination on the repair of a noncritical bone defect model. Twenty-four r...
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| Veröffentlicht in: | Acta Cirúrgica Brasileira 2023-01, Vol.38, p.e381623 |
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| container_title | Acta Cirúrgica Brasileira |
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| creator | Domínguez-Hernández, Victor Manuel Hernández-Flores, Cecília Delgado, Alfonso Valdez-Mijares, Rene Araujo-Monsalvo, Victor M Hernández-González, Olivia |
| description | Bone repair aims to restore the anatomical, biomechanical, and functional integrity of the affected structure. Here we study the effects of ascorbic acid (AA) and epidermal growth factor (EGF) applied in a single dose and in combination on the repair of a noncritical bone defect model.
Twenty-four rats were divided into four groups: an intact G-1 control group, and three groups that underwent a noncritical bone defect in the right tibia: G-2 treated with AA, G-3 treated with EGF, and G-4 treated with AA in combination with EGF. After 21 days of treatment, rats were sacrificed, the tibias were dissected and a destructive biomechanical analysis of three-point flexion test was performed in a universal testing machine; the values of stiffness, resistance, maximum energy, and energy at maximum load were statistically compared.
G-3 and G-4 recovered the biomechanical properties of strength and stiffness of an intact tibia 3 weeks after their application. Not so the energy and energy at maximum load. For G-2, only the stiffness of an intact tibia was recovered.
EGF and AA-EGF applied to a noncritical bone defect in the rat tibia favors the recovery of bone resistance and stiffness. |
| doi_str_mv | 10.1590/acb381623 |
| format | Article |
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Twenty-four rats were divided into four groups: an intact G-1 control group, and three groups that underwent a noncritical bone defect in the right tibia: G-2 treated with AA, G-3 treated with EGF, and G-4 treated with AA in combination with EGF. After 21 days of treatment, rats were sacrificed, the tibias were dissected and a destructive biomechanical analysis of three-point flexion test was performed in a universal testing machine; the values of stiffness, resistance, maximum energy, and energy at maximum load were statistically compared.
G-3 and G-4 recovered the biomechanical properties of strength and stiffness of an intact tibia 3 weeks after their application. Not so the energy and energy at maximum load. For G-2, only the stiffness of an intact tibia was recovered.
EGF and AA-EGF applied to a noncritical bone defect in the rat tibia favors the recovery of bone resistance and stiffness.</description><identifier>ISSN: 0102-8650</identifier><identifier>EISSN: 1678-2674</identifier><identifier>DOI: 10.1590/acb381623</identifier><identifier>PMID: 37132758</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia</publisher><subject>Animals ; Ascorbic Acid - pharmacology ; Ascorbic Acid - therapeutic use ; Biocompatible Materials ; Biomechanical Phenomena ; Epidermal Growth Factor - pharmacology ; Epidermal Growth Factor - therapeutic use ; Original ; Orthopedic Procedures ; Rats ; Tibia</subject><ispartof>Acta Cirúrgica Brasileira, 2023-01, Vol.38, p.e381623</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c441t-440929e356394280db9754116429c407b883a523e911103bd61b4d1d550dad293</cites><orcidid>0000-0003-1343-4732 ; 0000-0002-6969-0965 ; 0000-0001-6115-3287 ; 0000-0001-8922-7006 ; 0000-0002-5476-1425 ; 0000-0001-7920-9015</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159014/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10159014/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37132758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domínguez-Hernández, Victor Manuel</creatorcontrib><creatorcontrib>Hernández-Flores, Cecília</creatorcontrib><creatorcontrib>Delgado, Alfonso</creatorcontrib><creatorcontrib>Valdez-Mijares, Rene</creatorcontrib><creatorcontrib>Araujo-Monsalvo, Victor M</creatorcontrib><creatorcontrib>Hernández-González, Olivia</creatorcontrib><title>Effect of ascorbic acid and epidermal growth factor in a rat tibia defect</title><title>Acta Cirúrgica Brasileira</title><addtitle>Acta Cir Bras</addtitle><description>Bone repair aims to restore the anatomical, biomechanical, and functional integrity of the affected structure. Here we study the effects of ascorbic acid (AA) and epidermal growth factor (EGF) applied in a single dose and in combination on the repair of a noncritical bone defect model.
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G-3 and G-4 recovered the biomechanical properties of strength and stiffness of an intact tibia 3 weeks after their application. Not so the energy and energy at maximum load. For G-2, only the stiffness of an intact tibia was recovered.
EGF and AA-EGF applied to a noncritical bone defect in the rat tibia favors the recovery of bone resistance and stiffness.</description><subject>Animals</subject><subject>Ascorbic Acid - pharmacology</subject><subject>Ascorbic Acid - therapeutic use</subject><subject>Biocompatible Materials</subject><subject>Biomechanical Phenomena</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Epidermal Growth Factor - therapeutic use</subject><subject>Original</subject><subject>Orthopedic Procedures</subject><subject>Rats</subject><subject>Tibia</subject><issn>0102-8650</issn><issn>1678-2674</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNpVkc1LAzEQxYMoWqsH_wHJ1cNqJl-7OYlI1ULBi57D5GNrpG1KdlX87-1aLXoamPfmN_AeIWfALkEZdoXeiQY0F3tkBLpuKq5ruU9GDBivGq3YETnuulfGQGoQh-RI1CB4rZoRmU7aNvqe5pZi53NxyVP0KVBcBRrXKcSyxAWdl_zRv9AWfZ8LTSuKtGBP--QS0hAHxAk5aHHRxdOfOSbPd5On24dq9ng_vb2ZVV5K6CspmeEmCqWFkbxhwZlaSQAtufGS1a5pBCouogEAJlzQ4GSAoBQLGLgRYzLdckPGV7suaYnl02ZM9nuRy9xi6ZNfRGtcUCAk1KiMNFoiC6ZxSjsR27ARNqzrLWv95pYx-LjqCy7-Qf8rq_Ri5_ndAhuCh4FwsSX4kruuxHZ3DMwOHrsrZ-M9__tt5_xtQ3wB03CH9w</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Domínguez-Hernández, Victor Manuel</creator><creator>Hernández-Flores, Cecília</creator><creator>Delgado, Alfonso</creator><creator>Valdez-Mijares, Rene</creator><creator>Araujo-Monsalvo, Victor M</creator><creator>Hernández-González, Olivia</creator><general>Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1343-4732</orcidid><orcidid>https://orcid.org/0000-0002-6969-0965</orcidid><orcidid>https://orcid.org/0000-0001-6115-3287</orcidid><orcidid>https://orcid.org/0000-0001-8922-7006</orcidid><orcidid>https://orcid.org/0000-0002-5476-1425</orcidid><orcidid>https://orcid.org/0000-0001-7920-9015</orcidid></search><sort><creationdate>20230101</creationdate><title>Effect of ascorbic acid and epidermal growth factor in a rat tibia defect</title><author>Domínguez-Hernández, Victor Manuel ; Hernández-Flores, Cecília ; Delgado, Alfonso ; Valdez-Mijares, Rene ; Araujo-Monsalvo, Victor M ; Hernández-González, Olivia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-440929e356394280db9754116429c407b883a523e911103bd61b4d1d550dad293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Ascorbic Acid - pharmacology</topic><topic>Ascorbic Acid - therapeutic use</topic><topic>Biocompatible Materials</topic><topic>Biomechanical Phenomena</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Epidermal Growth Factor - therapeutic use</topic><topic>Original</topic><topic>Orthopedic Procedures</topic><topic>Rats</topic><topic>Tibia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domínguez-Hernández, Victor Manuel</creatorcontrib><creatorcontrib>Hernández-Flores, Cecília</creatorcontrib><creatorcontrib>Delgado, Alfonso</creatorcontrib><creatorcontrib>Valdez-Mijares, Rene</creatorcontrib><creatorcontrib>Araujo-Monsalvo, Victor M</creatorcontrib><creatorcontrib>Hernández-González, Olivia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Acta Cirúrgica Brasileira</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domínguez-Hernández, Victor Manuel</au><au>Hernández-Flores, Cecília</au><au>Delgado, Alfonso</au><au>Valdez-Mijares, Rene</au><au>Araujo-Monsalvo, Victor M</au><au>Hernández-González, Olivia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of ascorbic acid and epidermal growth factor in a rat tibia defect</atitle><jtitle>Acta Cirúrgica Brasileira</jtitle><addtitle>Acta Cir Bras</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>38</volume><spage>e381623</spage><pages>e381623-</pages><issn>0102-8650</issn><eissn>1678-2674</eissn><abstract>Bone repair aims to restore the anatomical, biomechanical, and functional integrity of the affected structure. Here we study the effects of ascorbic acid (AA) and epidermal growth factor (EGF) applied in a single dose and in combination on the repair of a noncritical bone defect model.
Twenty-four rats were divided into four groups: an intact G-1 control group, and three groups that underwent a noncritical bone defect in the right tibia: G-2 treated with AA, G-3 treated with EGF, and G-4 treated with AA in combination with EGF. After 21 days of treatment, rats were sacrificed, the tibias were dissected and a destructive biomechanical analysis of three-point flexion test was performed in a universal testing machine; the values of stiffness, resistance, maximum energy, and energy at maximum load were statistically compared.
G-3 and G-4 recovered the biomechanical properties of strength and stiffness of an intact tibia 3 weeks after their application. Not so the energy and energy at maximum load. For G-2, only the stiffness of an intact tibia was recovered.
EGF and AA-EGF applied to a noncritical bone defect in the rat tibia favors the recovery of bone resistance and stiffness.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia</pub><pmid>37132758</pmid><doi>10.1590/acb381623</doi><orcidid>https://orcid.org/0000-0003-1343-4732</orcidid><orcidid>https://orcid.org/0000-0002-6969-0965</orcidid><orcidid>https://orcid.org/0000-0001-6115-3287</orcidid><orcidid>https://orcid.org/0000-0001-8922-7006</orcidid><orcidid>https://orcid.org/0000-0002-5476-1425</orcidid><orcidid>https://orcid.org/0000-0001-7920-9015</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Animals Ascorbic Acid - pharmacology Ascorbic Acid - therapeutic use Biocompatible Materials Biomechanical Phenomena Epidermal Growth Factor - pharmacology Epidermal Growth Factor - therapeutic use Original Orthopedic Procedures Rats Tibia |
| title | Effect of ascorbic acid and epidermal growth factor in a rat tibia defect |
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