Linking Pedobacter lusitanus NL19 volatile exometabolome with growth medium composition: what can we learn using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry?
Microbial metabolomics allows understanding and to comprehensively analyse metabolites, and their related cellular and metabolic processes, that are produced and released to the extracellular environment under specific conditions. In that regard, the main objective of this research is to understand...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2023-05, Vol.415 (13), p.2613-2627 |
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| creator | Figueiredo, Gonçalo Costa, Carina Pedrosa Lourenço, Joana Caetano, Tânia Rocha, Sílvia M. Mendo, Sónia |
| description | Microbial metabolomics allows understanding and to comprehensively analyse metabolites, and their related cellular and metabolic processes, that are produced and released to the extracellular environment under specific conditions. In that regard, the main objective of this research is to understand the impact of culture media changes in the metabolic profile of
Pedobacter lusitanus
NL19 (NL19) and
Pedobacter himalayensis
MTCC 6384 (MTCC6384) and respective influence on the production of biotechnologically relevant compounds. Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry with time-of-flight analyser (GC × GC-ToFMS) was applied to comprehensively study the metabolites produced by NL19 and MTCC6384 both in tryptic soy broth 100% (TSB100) and tryptic soy broth with 25% casein peptone (PC25). A total of 320 metabolites were putatively identified, which belong to different chemical families: alcohols, aldehydes, esters, ethers, hydrocarbons, ketones, nitrogen compounds, sulphur compounds, monoterpenes, and sesquiterpenes. Metabolites that were statistically different from the control (sterile medium) were selected allowing for the construction of the metabolic profile of both strains. A set of 80 metabolites was tentatively associated to the metabolic pathways such as the metabolism of fatty acids, branched-chain aminoacids, phenylalanine, methionine, aromatic compounds, and monoterpene and sesquiterpene biosynthesis. This study allowed to better understand how slight changes of the culture media and thus the composition of nutrients impair the metabolic profile of bacteria, which may be further explored for metabolomics pipeline construction or biotechnological applications. |
| doi_str_mv | 10.1007/s00216-022-04505-6 |
| format | Article |
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Pedobacter lusitanus
NL19 (NL19) and
Pedobacter himalayensis
MTCC 6384 (MTCC6384) and respective influence on the production of biotechnologically relevant compounds. Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry with time-of-flight analyser (GC × GC-ToFMS) was applied to comprehensively study the metabolites produced by NL19 and MTCC6384 both in tryptic soy broth 100% (TSB100) and tryptic soy broth with 25% casein peptone (PC25). A total of 320 metabolites were putatively identified, which belong to different chemical families: alcohols, aldehydes, esters, ethers, hydrocarbons, ketones, nitrogen compounds, sulphur compounds, monoterpenes, and sesquiterpenes. Metabolites that were statistically different from the control (sterile medium) were selected allowing for the construction of the metabolic profile of both strains. A set of 80 metabolites was tentatively associated to the metabolic pathways such as the metabolism of fatty acids, branched-chain aminoacids, phenylalanine, methionine, aromatic compounds, and monoterpene and sesquiterpene biosynthesis. This study allowed to better understand how slight changes of the culture media and thus the composition of nutrients impair the metabolic profile of bacteria, which may be further explored for metabolomics pipeline construction or biotechnological applications.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-022-04505-6</identifier><identifier>PMID: 36631573</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Alcohols ; Aldehydes ; Analytical Chemistry ; Aromatic compounds ; Biochemistry ; Biosynthesis ; Biotechnology ; Casein ; Cell culture ; Chain branching ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Composition ; Comprehensive 2D Chromatography ; Culture Media ; Esters ; Ethers ; Fatty acids ; Food Science ; Gas chromatography ; Gas Chromatography-Mass Spectrometry - methods ; Humans ; Ions ; Ketones ; Laboratory Medicine ; Mass Spectrometry ; Mass spectroscopy ; Metabolic pathways ; Metabolism ; Metabolites ; Metabolomics ; Methionine ; Microorganisms ; Monitoring/Environmental Analysis ; Monoterpenes ; Nitrogen compounds ; Nutrients ; Pedobacter ; Peptones ; Phenylalanine ; Pipeline construction ; Research Paper ; Scientific imaging ; Sesquiterpenes ; Solid phase methods ; Solid Phase Microextraction - methods ; Solid phases ; Sulfur compounds ; Volatile Organic Compounds - chemistry</subject><ispartof>Analytical and bioanalytical chemistry, 2023-05, Vol.415 (13), p.2613-2627</ispartof><rights>The Author(s) 2022</rights><rights>2022. The Author(s).</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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In that regard, the main objective of this research is to understand the impact of culture media changes in the metabolic profile of
Pedobacter lusitanus
NL19 (NL19) and
Pedobacter himalayensis
MTCC 6384 (MTCC6384) and respective influence on the production of biotechnologically relevant compounds. Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry with time-of-flight analyser (GC × GC-ToFMS) was applied to comprehensively study the metabolites produced by NL19 and MTCC6384 both in tryptic soy broth 100% (TSB100) and tryptic soy broth with 25% casein peptone (PC25). A total of 320 metabolites were putatively identified, which belong to different chemical families: alcohols, aldehydes, esters, ethers, hydrocarbons, ketones, nitrogen compounds, sulphur compounds, monoterpenes, and sesquiterpenes. Metabolites that were statistically different from the control (sterile medium) were selected allowing for the construction of the metabolic profile of both strains. A set of 80 metabolites was tentatively associated to the metabolic pathways such as the metabolism of fatty acids, branched-chain aminoacids, phenylalanine, methionine, aromatic compounds, and monoterpene and sesquiterpene biosynthesis. This study allowed to better understand how slight changes of the culture media and thus the composition of nutrients impair the metabolic profile of bacteria, which may be further explored for metabolomics pipeline construction or biotechnological applications.</description><subject>Alcohols</subject><subject>Aldehydes</subject><subject>Analytical Chemistry</subject><subject>Aromatic compounds</subject><subject>Biochemistry</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Casein</subject><subject>Cell culture</subject><subject>Chain branching</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Composition</subject><subject>Comprehensive 2D Chromatography</subject><subject>Culture Media</subject><subject>Esters</subject><subject>Ethers</subject><subject>Fatty acids</subject><subject>Food Science</subject><subject>Gas 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Pedobacter lusitanus NL19 volatile exometabolome with growth medium composition: what can we learn using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry?</title><author>Figueiredo, Gonçalo ; Costa, Carina Pedrosa ; Lourenço, Joana ; Caetano, Tânia ; Rocha, Sílvia M. ; Mendo, Sónia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-32191d443a7037b457e95a40961f0db007fb2579795e4683dc57e0ba051fa2e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alcohols</topic><topic>Aldehydes</topic><topic>Analytical Chemistry</topic><topic>Aromatic compounds</topic><topic>Biochemistry</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Casein</topic><topic>Cell culture</topic><topic>Chain branching</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials 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exometabolome with growth medium composition: what can we learn using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry?</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2023-05-01</date><risdate>2023</risdate><volume>415</volume><issue>13</issue><spage>2613</spage><epage>2627</epage><pages>2613-2627</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Microbial metabolomics allows understanding and to comprehensively analyse metabolites, and their related cellular and metabolic processes, that are produced and released to the extracellular environment under specific conditions. In that regard, the main objective of this research is to understand the impact of culture media changes in the metabolic profile of
Pedobacter lusitanus
NL19 (NL19) and
Pedobacter himalayensis
MTCC 6384 (MTCC6384) and respective influence on the production of biotechnologically relevant compounds. Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry with time-of-flight analyser (GC × GC-ToFMS) was applied to comprehensively study the metabolites produced by NL19 and MTCC6384 both in tryptic soy broth 100% (TSB100) and tryptic soy broth with 25% casein peptone (PC25). A total of 320 metabolites were putatively identified, which belong to different chemical families: alcohols, aldehydes, esters, ethers, hydrocarbons, ketones, nitrogen compounds, sulphur compounds, monoterpenes, and sesquiterpenes. Metabolites that were statistically different from the control (sterile medium) were selected allowing for the construction of the metabolic profile of both strains. A set of 80 metabolites was tentatively associated to the metabolic pathways such as the metabolism of fatty acids, branched-chain aminoacids, phenylalanine, methionine, aromatic compounds, and monoterpene and sesquiterpene biosynthesis. This study allowed to better understand how slight changes of the culture media and thus the composition of nutrients impair the metabolic profile of bacteria, which may be further explored for metabolomics pipeline construction or biotechnological applications.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36631573</pmid><doi>10.1007/s00216-022-04505-6</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-0757-187X</orcidid><orcidid>https://orcid.org/0000-0002-0396-3019</orcidid><orcidid>https://orcid.org/0000-0002-3732-774X</orcidid><orcidid>https://orcid.org/0000-0003-0301-6385</orcidid><orcidid>https://orcid.org/0000-0001-9157-8761</orcidid><orcidid>https://orcid.org/0000-0001-5057-5660</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2023-05, Vol.415 (13), p.2613-2627 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10149447 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Alcohols Aldehydes Analytical Chemistry Aromatic compounds Biochemistry Biosynthesis Biotechnology Casein Cell culture Chain branching Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography Composition Comprehensive 2D Chromatography Culture Media Esters Ethers Fatty acids Food Science Gas chromatography Gas Chromatography-Mass Spectrometry - methods Humans Ions Ketones Laboratory Medicine Mass Spectrometry Mass spectroscopy Metabolic pathways Metabolism Metabolites Metabolomics Methionine Microorganisms Monitoring/Environmental Analysis Monoterpenes Nitrogen compounds Nutrients Pedobacter Peptones Phenylalanine Pipeline construction Research Paper Scientific imaging Sesquiterpenes Solid phase methods Solid Phase Microextraction - methods Solid phases Sulfur compounds Volatile Organic Compounds - chemistry |
| title | Linking Pedobacter lusitanus NL19 volatile exometabolome with growth medium composition: what can we learn using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry? |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T00%3A01%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Linking%20Pedobacter%20lusitanus%20NL19%20volatile%20exometabolome%20with%20growth%20medium%20composition:%20what%20can%20we%20learn%20using%20comprehensive%20two-dimensional%20gas%20chromatography%20coupled%20to%20time-of-flight%20mass%20spectrometry?&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Figueiredo,%20Gon%C3%A7alo&rft.date=2023-05-01&rft.volume=415&rft.issue=13&rft.spage=2613&rft.epage=2627&rft.pages=2613-2627&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-022-04505-6&rft_dat=%3Cproquest_pubme%3E2765071546%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2807781360&rft_id=info:pmid/36631573&rfr_iscdi=true |