Influence of Fabrication Technique on Adhesion and Biofilm Formation of Candida albicans to Conventional, Milled, and 3D-Printed Denture Base Resin Materials: A Comparative In Vitro Study
The aim of this study was to evaluate the adhesion and biofilm formation of ( ) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamination during clinical use. Specimens were incubated with (ATCC 10231) for 1 an...
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| Veröffentlicht in: | Polymers 2023-04, Vol.15 (8), p.1836 |
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| creator | Osman, Reham B Khoder, Ghalia Fayed, Bahgat Kedia, Reena Arora Elkareimi, Yaser Alharbi, Nawal |
| description | The aim of this study was to evaluate the adhesion and biofilm formation of
(
) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamination during clinical use. Specimens were incubated with
(ATCC 10231) for 1 and 24 h. Adhesion and biofilm formation of
were assessed using the field emission scanning electron microscopy (FESEM). The XTT (2,3-(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) assay was used for the quantification of fungal adhesion and biofilm formation. The data were analyzed using GraphPad Prism 8.02 for windows. One-way ANOVA with Tukey's post hoc testing were performed with a statistical significance level set at α = 0.05. The quantitative XTT biofilm assay revealed significant differences in the biofilm formation of
between the three groups in the 24 h incubation period. The highest proportion of biofilm formation was observed in the 3D-printed group, followed by the conventional group, while the lowest candida biofilm formation was observed in the milled group. The difference in biofilm formation among the three tested dentures was statistically significant (
< 0.001). The manufacturing technique has an influence on the surface topography and microbiological properties of the fabricated denture base resin material. Additive 3D-printing technology results in increased candida adhesion and the roughest surface topography of maxillary resin denture base as compared to conventional flask compression and CAD/CAM milling techniques. In a clinical setting, patients wearing additively manufactured maxillary complete dentures are thus more susceptible to the development of candida-associated denture stomatitis and accordingly, strict oral hygiene measures and maintenance programs should be emphasized to patients. |
| doi_str_mv | 10.3390/polym15081836 |
| format | Article |
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(
) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamination during clinical use. Specimens were incubated with
(ATCC 10231) for 1 and 24 h. Adhesion and biofilm formation of
were assessed using the field emission scanning electron microscopy (FESEM). The XTT (2,3-(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) assay was used for the quantification of fungal adhesion and biofilm formation. The data were analyzed using GraphPad Prism 8.02 for windows. One-way ANOVA with Tukey's post hoc testing were performed with a statistical significance level set at α = 0.05. The quantitative XTT biofilm assay revealed significant differences in the biofilm formation of
between the three groups in the 24 h incubation period. The highest proportion of biofilm formation was observed in the 3D-printed group, followed by the conventional group, while the lowest candida biofilm formation was observed in the milled group. The difference in biofilm formation among the three tested dentures was statistically significant (
< 0.001). The manufacturing technique has an influence on the surface topography and microbiological properties of the fabricated denture base resin material. Additive 3D-printing technology results in increased candida adhesion and the roughest surface topography of maxillary resin denture base as compared to conventional flask compression and CAD/CAM milling techniques. In a clinical setting, patients wearing additively manufactured maxillary complete dentures are thus more susceptible to the development of candida-associated denture stomatitis and accordingly, strict oral hygiene measures and maintenance programs should be emphasized to patients.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym15081836</identifier><identifier>PMID: 37111983</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biofilms ; CAD/CAM ; CAM ; Carbonyls ; Computer aided manufacturing ; Dental materials ; Dentures ; Edentulous ; Emission analysis ; Field emission microscopy ; Heat ; Manufacturers ; Manufacturing ; Mechanical properties ; Oral hygiene ; Prostheses ; Prosthodontics ; Resins ; Three dimensional printing ; Topography</subject><ispartof>Polymers, 2023-04, Vol.15 (8), p.1836</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-a7fe167da9b07569aa9624badc2c2cbe06d163e1a021d89bcfdb81d67d472f753</citedby><cites>FETCH-LOGICAL-c416t-a7fe167da9b07569aa9624badc2c2cbe06d163e1a021d89bcfdb81d67d472f753</cites><orcidid>0000-0002-6036-1022 ; 0000-0003-0442-9325 ; 0000-0001-9101-3285</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146129/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10146129/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37111983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osman, Reham B</creatorcontrib><creatorcontrib>Khoder, Ghalia</creatorcontrib><creatorcontrib>Fayed, Bahgat</creatorcontrib><creatorcontrib>Kedia, Reena Arora</creatorcontrib><creatorcontrib>Elkareimi, Yaser</creatorcontrib><creatorcontrib>Alharbi, Nawal</creatorcontrib><title>Influence of Fabrication Technique on Adhesion and Biofilm Formation of Candida albicans to Conventional, Milled, and 3D-Printed Denture Base Resin Materials: A Comparative In Vitro Study</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>The aim of this study was to evaluate the adhesion and biofilm formation of
(
) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamination during clinical use. Specimens were incubated with
(ATCC 10231) for 1 and 24 h. Adhesion and biofilm formation of
were assessed using the field emission scanning electron microscopy (FESEM). The XTT (2,3-(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) assay was used for the quantification of fungal adhesion and biofilm formation. The data were analyzed using GraphPad Prism 8.02 for windows. One-way ANOVA with Tukey's post hoc testing were performed with a statistical significance level set at α = 0.05. The quantitative XTT biofilm assay revealed significant differences in the biofilm formation of
between the three groups in the 24 h incubation period. The highest proportion of biofilm formation was observed in the 3D-printed group, followed by the conventional group, while the lowest candida biofilm formation was observed in the milled group. The difference in biofilm formation among the three tested dentures was statistically significant (
< 0.001). The manufacturing technique has an influence on the surface topography and microbiological properties of the fabricated denture base resin material. Additive 3D-printing technology results in increased candida adhesion and the roughest surface topography of maxillary resin denture base as compared to conventional flask compression and CAD/CAM milling techniques. In a clinical setting, patients wearing additively manufactured maxillary complete dentures are thus more susceptible to the development of candida-associated denture stomatitis and accordingly, strict oral hygiene measures and maintenance programs should be emphasized to patients.</description><subject>Biofilms</subject><subject>CAD/CAM</subject><subject>CAM</subject><subject>Carbonyls</subject><subject>Computer aided manufacturing</subject><subject>Dental materials</subject><subject>Dentures</subject><subject>Edentulous</subject><subject>Emission analysis</subject><subject>Field emission microscopy</subject><subject>Heat</subject><subject>Manufacturers</subject><subject>Manufacturing</subject><subject>Mechanical properties</subject><subject>Oral hygiene</subject><subject>Prostheses</subject><subject>Prosthodontics</subject><subject>Resins</subject><subject>Three dimensional printing</subject><subject>Topography</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkk1v1DAQhiMEolXpkSuyxIVDA_5I7IQL2m67sFIrUFu4RpN4wrpy7MVOVtrfxp-rt1uqFvtgy-8z74xHk2VvGf0oRE0_rb3dDqykFauEfJEdcqpEXghJXz65H2THMd7StIpSSqZeZwdCMcbqShxmf5eutxO6DonvyQLaYDoYjXfkBruVM3-mJDgy0yuMu1dwmpwa3xs7kIUPw55NofOkGA0EbJscXCSjJ3PvNuh2BNgTcmmsRX1ybyHO8h_BuBE1OUvEFJCcQkRylbI4cgkjBgM2fiazZDKsIaQ8GyRLR36ZMXhyPU56-yZ71ScIjx_Oo-zn4vxm_i2_-P51OZ9d5F3B5JiD6pFJpaFuqSplDVBLXrSgO552i1RqJgUyoJzpqm67XrcV0ymiULxXpTjKvux911M7oO5SwQFssw5mgLBtPJjmueLMqvntNw2jrJCM18nhw4ND8KmjcWwGEzu0Fhz6KTa8oqrmvFQsoe__Q2_9FFID7ylZ1oVUPFH5nuqCjzFg_1gNo81uNJpno5H4d0-_8Ej_GwRxB2EXt_k</recordid><startdate>20230410</startdate><enddate>20230410</enddate><creator>Osman, Reham B</creator><creator>Khoder, Ghalia</creator><creator>Fayed, Bahgat</creator><creator>Kedia, Reena Arora</creator><creator>Elkareimi, Yaser</creator><creator>Alharbi, Nawal</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6036-1022</orcidid><orcidid>https://orcid.org/0000-0003-0442-9325</orcidid><orcidid>https://orcid.org/0000-0001-9101-3285</orcidid></search><sort><creationdate>20230410</creationdate><title>Influence of Fabrication Technique on Adhesion and Biofilm Formation of Candida albicans to Conventional, Milled, and 3D-Printed Denture Base Resin Materials: A Comparative In Vitro Study</title><author>Osman, Reham B ; 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(
) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamination during clinical use. Specimens were incubated with
(ATCC 10231) for 1 and 24 h. Adhesion and biofilm formation of
were assessed using the field emission scanning electron microscopy (FESEM). The XTT (2,3-(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) assay was used for the quantification of fungal adhesion and biofilm formation. The data were analyzed using GraphPad Prism 8.02 for windows. One-way ANOVA with Tukey's post hoc testing were performed with a statistical significance level set at α = 0.05. The quantitative XTT biofilm assay revealed significant differences in the biofilm formation of
between the three groups in the 24 h incubation period. The highest proportion of biofilm formation was observed in the 3D-printed group, followed by the conventional group, while the lowest candida biofilm formation was observed in the milled group. The difference in biofilm formation among the three tested dentures was statistically significant (
< 0.001). The manufacturing technique has an influence on the surface topography and microbiological properties of the fabricated denture base resin material. Additive 3D-printing technology results in increased candida adhesion and the roughest surface topography of maxillary resin denture base as compared to conventional flask compression and CAD/CAM milling techniques. In a clinical setting, patients wearing additively manufactured maxillary complete dentures are thus more susceptible to the development of candida-associated denture stomatitis and accordingly, strict oral hygiene measures and maintenance programs should be emphasized to patients.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37111983</pmid><doi>10.3390/polym15081836</doi><orcidid>https://orcid.org/0000-0002-6036-1022</orcidid><orcidid>https://orcid.org/0000-0003-0442-9325</orcidid><orcidid>https://orcid.org/0000-0001-9101-3285</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Biofilms CAD/CAM CAM Carbonyls Computer aided manufacturing Dental materials Dentures Edentulous Emission analysis Field emission microscopy Heat Manufacturers Manufacturing Mechanical properties Oral hygiene Prostheses Prosthodontics Resins Three dimensional printing Topography |
| title | Influence of Fabrication Technique on Adhesion and Biofilm Formation of Candida albicans to Conventional, Milled, and 3D-Printed Denture Base Resin Materials: A Comparative In Vitro Study |
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