Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification

Elephant endotheliotropic herpesvirus (EEHV) causes a fatal hemorrhagic disease and is a significant cause of mortality in juvenile Asian elephants. A loop-mediated isothermal amplification (LAMP) method was developed to rapidly diagnose EEHV viremia. However, extracting DNA from whole blood samples...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2023, Vol.85(4), pp.459-462
Hauptverfasser:	TAKEHANA, Kazuya, MATSUNO, Keita
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Sprache:	eng
Schlagworte:	Animals Asian elephants (Elephas maximus) Deoxyribonucleic acid Diagnosis direct detection DNA elephant endotheliotropic herpes virus (EEHV) Elephants Gene amplification Hemorrhagic disease heparinized plasma Herpes viruses Herpesviridae - genetics Herpesviridae Infections - diagnosis Herpesviridae Infections - veterinary loop-mediated isothermal amplification (LAMP) Viremia Wildlife Science
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creator	TAKEHANA, Kazuya MATSUNO, Keita
description	Elephant endotheliotropic herpesvirus (EEHV) causes a fatal hemorrhagic disease and is a significant cause of mortality in juvenile Asian elephants. A loop-mediated isothermal amplification (LAMP) method was developed to rapidly diagnose EEHV viremia. However, extracting DNA from whole blood samples to perform LAMP hampers diagnosis in a field setting. Here, we established the Direct-LAMP method, using heparinized plasma without extracting the DNA to speed up and simplify the test. EEHV-positive specimens were tested using the Direct-LAMP. The detection limit was calculated to be 101.3 copies/μL using the mimetic samples, which was almost identical to the value determined in LAMP in which DNA was extracted. Hence, the Direct-LAMP provided a more rapid diagnosis to save, which could prevent elephant deaths.
doi_str_mv	10.1292/jvms.22-0578
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Vet. Med. Sci.</addtitle><description>Elephant endotheliotropic herpesvirus (EEHV) causes a fatal hemorrhagic disease and is a significant cause of mortality in juvenile Asian elephants. A loop-mediated isothermal amplification (LAMP) method was developed to rapidly diagnose EEHV viremia. However, extracting DNA from whole blood samples to perform LAMP hampers diagnosis in a field setting. Here, we established the Direct-LAMP method, using heparinized plasma without extracting the DNA to speed up and simplify the test. EEHV-positive specimens were tested using the Direct-LAMP. The detection limit was calculated to be 101.3 copies/μL using the mimetic samples, which was almost identical to the value determined in LAMP in which DNA was extracted. Hence, the Direct-LAMP provided a more rapid diagnosis to save, which could prevent elephant deaths.</description><subject>Animals</subject><subject>Asian elephants (Elephas maximus)</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>direct detection</subject><subject>DNA</subject><subject>elephant endotheliotropic herpes virus (EEHV)</subject><subject>Elephants</subject><subject>Gene amplification</subject><subject>Hemorrhagic disease</subject><subject>heparinized plasma</subject><subject>Herpes viruses</subject><subject>Herpesviridae - genetics</subject><subject>Herpesviridae Infections - diagnosis</subject><subject>Herpesviridae Infections - veterinary</subject><subject>loop-mediated isothermal amplification (LAMP)</subject><subject>Viremia</subject><subject>Wildlife Science</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUFv1DAQhSMEotvCjTOyxKVIpNhOYscnVNqFIlVwAa7WxOs0Xjl2ajsrlT_Qv42jXVaALHkkv09vZvyK4hXBF4QK-n67G-MFpSVuePukWJGq5iWvK_G0WGFBWMlpg0-K0xi3GFNSM_G8OKkYF5QSvCoer03QKqGNTrkY75DvkbZ6GsAlpN3Gp0Fb41Pwk1Fo0GHScWfCHBFB5-v1zU_yFl1_vUTGZXGCYJz5pTdoshBHQN0Dst5P5ag3BlJ-N3ExDCNYBONkTW8ULG1fFM96sFG_PNSz4sen9ferm_L22-cvV5e3pWo4SyX0tGYdbltGq7rPh3cN7RRrMO2ZaIRqFQWlgAvMoWshfxDFte4wCCC4qqqz4sPed5q7PJTSLgWwcgpmhPAgPRj5r-LMIO_8ThJMKsFblh3ODw7B3886JjmaqLS14LSfo6Sc8xozRkRG3_yHbv0cXN5P0jYHJYigPFPv9pQKPsag--M0BMslYrlELCmVS8QZf_33Bkf4T6YZ-LgHtjHBnT4CEJJRVu_d2kbWy3VwPYpqgCC1q34DnBu8RA</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>TAKEHANA, Kazuya</creator><creator>MATSUNO, Keita</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2023</creationdate><title>Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification</title><author>TAKEHANA, Kazuya ; MATSUNO, Keita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-af246b0886234f4f47b52bc6502f6959c8c2acca7907ab8a292204eb0a9a10333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Asian elephants (Elephas maximus)</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>direct detection</topic><topic>DNA</topic><topic>elephant endotheliotropic herpes virus (EEHV)</topic><topic>Elephants</topic><topic>Gene amplification</topic><topic>Hemorrhagic disease</topic><topic>heparinized plasma</topic><topic>Herpes viruses</topic><topic>Herpesviridae - genetics</topic><topic>Herpesviridae Infections - diagnosis</topic><topic>Herpesviridae Infections - veterinary</topic><topic>loop-mediated isothermal amplification (LAMP)</topic><topic>Viremia</topic><topic>Wildlife Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAKEHANA, Kazuya</creatorcontrib><creatorcontrib>MATSUNO, Keita</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAKEHANA, Kazuya</au><au>MATSUNO, Keita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2023</date><risdate>2023</risdate><volume>85</volume><issue>4</issue><spage>459</spage><epage>462</epage><pages>459-462</pages><artnum>22-0578</artnum><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Elephant endotheliotropic herpesvirus (EEHV) causes a fatal hemorrhagic disease and is a significant cause of mortality in juvenile Asian elephants. A loop-mediated isothermal amplification (LAMP) method was developed to rapidly diagnose EEHV viremia. However, extracting DNA from whole blood samples to perform LAMP hampers diagnosis in a field setting. Here, we established the Direct-LAMP method, using heparinized plasma without extracting the DNA to speed up and simplify the test. EEHV-positive specimens were tested using the Direct-LAMP. The detection limit was calculated to be 101.3 copies/μL using the mimetic samples, which was almost identical to the value determined in LAMP in which DNA was extracted. 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subjects	Animals Asian elephants (Elephas maximus) Deoxyribonucleic acid Diagnosis direct detection DNA elephant endotheliotropic herpes virus (EEHV) Elephants Gene amplification Hemorrhagic disease heparinized plasma Herpes viruses Herpesviridae - genetics Herpesviridae Infections - diagnosis Herpesviridae Infections - veterinary loop-mediated isothermal amplification (LAMP) Viremia Wildlife Science
title	Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification
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