Expression of human papillomavirus 16 and 18 DNA in oral lichen planus using polymerase chain reaction
Introduction: Oral lichen planus (OLP) is a chronic inflammatory disease with cell-mediated immune dysregulation. The aetiology of OLP has been studied extensively for decades. Viruses like Hepatitis C Virus (HCV), human papillomavirus (HPV), herpes simplex virus (HSV), and stress have been hypothes...
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| Veröffentlicht in: | Journal of oral and maxillofacial pathology : JOMFP 2022-10, Vol.26 (4), p.495-500 |
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| container_title | Journal of oral and maxillofacial pathology : JOMFP |
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| creator | Vijayan, Aswathy Muthukrishnan, Arvind Velayudhannair, Vivek Varun, Jayanthi Vidyadharan, Mathew James, Joseena |
| description | Introduction: Oral lichen planus (OLP) is a chronic inflammatory disease with cell-mediated immune dysregulation. The aetiology of OLP has been studied extensively for decades. Viruses like Hepatitis C Virus (HCV), human papillomavirus (HPV), herpes simplex virus (HSV), and stress have been hypothesized to play a role in the pathogenesis and malignant transformation of OLP. HPV has been proved to be an etiological agent in oropharyngeal cancers and non-tobacco-associated leukoplakia. The role of human papillomavirus in the pathogenesis of OLP has to be studied extensively.
Aim: This study aims to detect the presence of HPV 16 and HPV 18 DNA in the biopsy samples of OLP and also to determine the role played by the virus in the pathogenesis and malignant transformation of OLP.
Materials and Methods: Biopsy samples comprising 30 OLP tissues were collected. The DNA was extracted by the cetyltrimethylammonium bromide method. Polymerase chain reaction was performed by using general primers to amplify the HPV E6 gene.
Results: Twelve out of 30 (40%) OLP cases were positive for HPV DNA. A significant relation was found between HPV, site (buccal mucosa) and the type (reticular) of the lesion (P = 0.007). However, the difference between the percentage of HPV positive males and females was statistically insignificant (P = 0.852).
Conclusion: This study confirmed the presence of high-risk HPV 16 and HPV 18 DNA in OLP. The study showed a significantly higher expression of HPV in erosive OLP when compared to reticular OLP, suggesting a possible role of HPV in the malignant transformation of OLP. |
| doi_str_mv | 10.4103/jomfp.jomfp_61_22 |
| format | Article |
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Aim: This study aims to detect the presence of HPV 16 and HPV 18 DNA in the biopsy samples of OLP and also to determine the role played by the virus in the pathogenesis and malignant transformation of OLP.
Materials and Methods: Biopsy samples comprising 30 OLP tissues were collected. The DNA was extracted by the cetyltrimethylammonium bromide method. Polymerase chain reaction was performed by using general primers to amplify the HPV E6 gene.
Results: Twelve out of 30 (40%) OLP cases were positive for HPV DNA. A significant relation was found between HPV, site (buccal mucosa) and the type (reticular) of the lesion (P = 0.007). However, the difference between the percentage of HPV positive males and females was statistically insignificant (P = 0.852).
Conclusion: This study confirmed the presence of high-risk HPV 16 and HPV 18 DNA in OLP. The study showed a significantly higher expression of HPV in erosive OLP when compared to reticular OLP, suggesting a possible role of HPV in the malignant transformation of OLP.</description><identifier>ISSN: 0973-029X</identifier><identifier>EISSN: 1998-393X</identifier><identifier>DOI: 10.4103/jomfp.jomfp_61_22</identifier><identifier>PMID: 37082079</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Biopsy ; Buccal mucosa ; Deoxyribonucleic acid ; DNA ; E6 gene ; Genetic transformation ; Head & neck cancer ; Hepatitis C ; Herpes simplex ; Human papillomavirus ; Inflammatory diseases ; Leukokeratosis ; Lichen planus ; Original ; Papillomavirus infections ; Papillomaviruses ; Pathogenesis ; Polymerase chain reaction ; Throat cancer ; Viruses</subject><ispartof>Journal of oral and maxillofacial pathology : JOMFP, 2022-10, Vol.26 (4), p.495-500</ispartof><rights>Copyright: © 2022 Journal of Oral and Maxillofacial Pathology.</rights><rights>COPYRIGHT 2022 Medknow Publications and Media Pvt. Ltd.</rights><rights>2022. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2022 Journal of Oral and Maxillofacial Pathology 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423i-2701122783f35a3addf823f0eccc097c00eda030ff122be30c29956509f8f7f03</citedby><cites>FETCH-LOGICAL-c423i-2701122783f35a3addf823f0eccc097c00eda030ff122be30c29956509f8f7f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10112116/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10112116/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27435,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37082079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vijayan, Aswathy</creatorcontrib><creatorcontrib>Muthukrishnan, Arvind</creatorcontrib><creatorcontrib>Velayudhannair, Vivek</creatorcontrib><creatorcontrib>Varun, Jayanthi</creatorcontrib><creatorcontrib>Vidyadharan, Mathew</creatorcontrib><creatorcontrib>James, Joseena</creatorcontrib><title>Expression of human papillomavirus 16 and 18 DNA in oral lichen planus using polymerase chain reaction</title><title>Journal of oral and maxillofacial pathology : JOMFP</title><addtitle>J Oral Maxillofac Pathol</addtitle><description>Introduction: Oral lichen planus (OLP) is a chronic inflammatory disease with cell-mediated immune dysregulation. The aetiology of OLP has been studied extensively for decades. Viruses like Hepatitis C Virus (HCV), human papillomavirus (HPV), herpes simplex virus (HSV), and stress have been hypothesized to play a role in the pathogenesis and malignant transformation of OLP. HPV has been proved to be an etiological agent in oropharyngeal cancers and non-tobacco-associated leukoplakia. The role of human papillomavirus in the pathogenesis of OLP has to be studied extensively.
Aim: This study aims to detect the presence of HPV 16 and HPV 18 DNA in the biopsy samples of OLP and also to determine the role played by the virus in the pathogenesis and malignant transformation of OLP.
Materials and Methods: Biopsy samples comprising 30 OLP tissues were collected. The DNA was extracted by the cetyltrimethylammonium bromide method. Polymerase chain reaction was performed by using general primers to amplify the HPV E6 gene.
Results: Twelve out of 30 (40%) OLP cases were positive for HPV DNA. A significant relation was found between HPV, site (buccal mucosa) and the type (reticular) of the lesion (P = 0.007). However, the difference between the percentage of HPV positive males and females was statistically insignificant (P = 0.852).
Conclusion: This study confirmed the presence of high-risk HPV 16 and HPV 18 DNA in OLP. The study showed a significantly higher expression of HPV in erosive OLP when compared to reticular OLP, suggesting a possible role of HPV in the malignant transformation of OLP.</description><subject>Biopsy</subject><subject>Buccal mucosa</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E6 gene</subject><subject>Genetic transformation</subject><subject>Head & neck cancer</subject><subject>Hepatitis C</subject><subject>Herpes simplex</subject><subject>Human papillomavirus</subject><subject>Inflammatory diseases</subject><subject>Leukokeratosis</subject><subject>Lichen planus</subject><subject>Original</subject><subject>Papillomavirus infections</subject><subject>Papillomaviruses</subject><subject>Pathogenesis</subject><subject>Polymerase chain reaction</subject><subject>Throat cancer</subject><subject>Viruses</subject><issn>0973-029X</issn><issn>1998-393X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kkFvFCEcxYnR2G31A3gxJF68zPoHhpnhZDZrqyaNXjTpjVAGdtkyMMJO1357WbetrdGQQAK_9-CRh9ArAvOaAHu3iYMd579n2RBJ6RM0I0J0FRPs4imagWhZBVRcHKHjnDcAvKs5fY6OWAsdhVbMkD39OSaTs4sBR4vX06ACHtXovI-DunZpypg0WIUekw5_-LLAroBJeeydXpvCehUKM2UXVniM_mYwSWWD9VoVMhmlt8X7BXpmlc_m5e16gr6fnX5bfqrOv378vFycV7qmzFW0BUIobTtmGVdM9b3tKLNgtNYljAYwvQIG1hbq0jDQVAjecBC2s60FdoLeH3zH6XIwvTZhW94qx-QGlW5kVE4-PgluLVfxWpL9xYQ0xeHtrUOKPyaTt3JwWRtfYpo4ZUk74EDbWtCCvvkL3cQphZJP0pZ3lHPaNH-olfJGumBjuVjvTeWiZaRQDPZe839QZfRmcDoGY13ZfyQgB4FOMedk7H1IAnLfDnnoxYN2FM3rh79zr7irQwGWB2AX_dakfOWnnUmysFch7v7vLGvB5V2T2C9C-9A1</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Vijayan, Aswathy</creator><creator>Muthukrishnan, Arvind</creator><creator>Velayudhannair, Vivek</creator><creator>Varun, Jayanthi</creator><creator>Vidyadharan, Mathew</creator><creator>James, Joseena</creator><general>Wolters Kluwer India Pvt. 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The aetiology of OLP has been studied extensively for decades. Viruses like Hepatitis C Virus (HCV), human papillomavirus (HPV), herpes simplex virus (HSV), and stress have been hypothesized to play a role in the pathogenesis and malignant transformation of OLP. HPV has been proved to be an etiological agent in oropharyngeal cancers and non-tobacco-associated leukoplakia. The role of human papillomavirus in the pathogenesis of OLP has to be studied extensively.
Aim: This study aims to detect the presence of HPV 16 and HPV 18 DNA in the biopsy samples of OLP and also to determine the role played by the virus in the pathogenesis and malignant transformation of OLP.
Materials and Methods: Biopsy samples comprising 30 OLP tissues were collected. The DNA was extracted by the cetyltrimethylammonium bromide method. Polymerase chain reaction was performed by using general primers to amplify the HPV E6 gene.
Results: Twelve out of 30 (40%) OLP cases were positive for HPV DNA. A significant relation was found between HPV, site (buccal mucosa) and the type (reticular) of the lesion (P = 0.007). However, the difference between the percentage of HPV positive males and females was statistically insignificant (P = 0.852).
Conclusion: This study confirmed the presence of high-risk HPV 16 and HPV 18 DNA in OLP. The study showed a significantly higher expression of HPV in erosive OLP when compared to reticular OLP, suggesting a possible role of HPV in the malignant transformation of OLP.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>37082079</pmid><doi>10.4103/jomfp.jomfp_61_22</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0973-029X 1998-393X |
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| source | Medknow Open Access Journals; PubMed Central; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Biopsy Buccal mucosa Deoxyribonucleic acid DNA E6 gene Genetic transformation Head & neck cancer Hepatitis C Herpes simplex Human papillomavirus Inflammatory diseases Leukokeratosis Lichen planus Original Papillomavirus infections Papillomaviruses Pathogenesis Polymerase chain reaction Throat cancer Viruses |
| title | Expression of human papillomavirus 16 and 18 DNA in oral lichen planus using polymerase chain reaction |
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