MINSTED nanoscopy enters the Ångström localization range

Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the fluor...

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Veröffentlicht in:	Nature biotechnology 2023-04, Vol.41 (4), p.569-576
Hauptverfasser:	Weber, Michael, von der Emde, Henrik, Leutenegger, Marcel, Gunkel, Philip, Sambandan, Sivakumar, Khan, Taukeer A., Keller-Findeisen, Jan, Cordes, Volker C., Hell, Stefan W.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/328/2238 639/624/1107/328/2238 Agriculture Animals Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Chemical compounds Deoxyribonucleic acid Depletion DNA Fluorescence Fluorescent Dyes Fluorophores Hybridization Life Sciences Light microscopy Localization Macromolecules Mammalian cells Mammals Microscopy, Fluorescence - methods Nuclear pores Optical microscopy Photons Room temperature Stimulated emission
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