A Multiplex Gene Expression Assay for Direct Measurement of RNA Transcripts in Crude Lysates of the Nematode Caenorhabditis elegans Used as a Bioanalytical Tool

Gene expression profiling in Caenorhabditis elegans has been demonstrated to be a potential bioanalytical tool to detect the toxic potency of environmental contaminants. The RNA transcripts of genes responding to toxic exposure can be used as biomarkers for detecting these toxins. For routine applic...

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Veröffentlicht in:	Environmental toxicology and chemistry 2023-01, Vol.42 (1), p.130-142
Hauptverfasser:	Karengera, Antoine, Bao, Cong, Bovee, Toine F. H., Dinkla, Inez J. T., Murk, Albertinka J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Animals Assaying Biological activity Biomarkers Caenorhabditis elegans Caenorhabditis elegans - genetics Contaminants Crustaceans DNA microarrays Environmental monitoring Environmental quality Environmental Toxicology Evaluation Fingerprints Fluorescence Gene expression Gene Expression Profiling Genes Lysates multiplex assay Multiplexing nematode bioassay Nematodes Pollutants Polymerase chain reaction Ribonucleic acid RNA RNA, Messenger - metabolism Swimming Toxicity Toxicology Toxins Transcriptome Transcriptomics Wastewater water quality Water sampling
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