Oestrogen and Vibration Improve Intervertebral Disc Cell Viability and Decrease Catabolism in Bovine Organ Cultures
Postmenopausal women are at an increased risk for intervertebral disc degeneration, possibly due to the decrease in oestrogen levels. Low-magnitude, high-frequency vibration (LMHFV) is applied as a therapeutic approach for postmenopausal osteoporosis; however, less is known regarding possible effect...
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| Veröffentlicht in: | International journal of molecular sciences 2023-03, Vol.24 (7), p.6143 |
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| creator | Widmayer, Franziska Neidlinger-Wilke, Cornelia Witz, Fiona Jansen, Jan U Ignatius, Anita Haffner-Luntzer, Melanie Teixeira, Graciosa Q |
| description | Postmenopausal women are at an increased risk for intervertebral disc degeneration, possibly due to the decrease in oestrogen levels. Low-magnitude, high-frequency vibration (LMHFV) is applied as a therapeutic approach for postmenopausal osteoporosis; however, less is known regarding possible effects on the intervertebral disc (IVD) and whether these may be oestrogen-dependent. The present study investigated the effect of 17β-oestradiol (E2) and LMHFV in an IVD organ culture model. Bovine IVDs (
= 6 IVDs/group) were treated with either (i) E2, (ii) LMHFV or (iii) the combination of E2 + LMHFV for 2 or 14 days. Minor changes in gene expression, cellularity and matrix metabolism were observed after E2 treatment, except for a significant increase in matrix metalloproteinase (MMP)-3 and interleukin (IL)-6 production. Interestingly, LMHFV alone induced cell loss and increased IL-6 production compared to the control. The combination of E2 + LMHFV induced a protective effect against cell loss and decreased IL-6 production compared to the LMHFV group. This indicates possible benefits of oestrogen therapy for the IVDs of postmenopausal women undergoing LMHFV exercises. |
| doi_str_mv | 10.3390/ijms24076143 |
| format | Article |
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= 6 IVDs/group) were treated with either (i) E2, (ii) LMHFV or (iii) the combination of E2 + LMHFV for 2 or 14 days. Minor changes in gene expression, cellularity and matrix metabolism were observed after E2 treatment, except for a significant increase in matrix metalloproteinase (MMP)-3 and interleukin (IL)-6 production. Interestingly, LMHFV alone induced cell loss and increased IL-6 production compared to the control. The combination of E2 + LMHFV induced a protective effect against cell loss and decreased IL-6 production compared to the LMHFV group. This indicates possible benefits of oestrogen therapy for the IVDs of postmenopausal women undergoing LMHFV exercises.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms24076143</identifier><identifier>PMID: 37047116</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Apoptosis ; Back pain ; Catabolism ; Cattle ; Cell culture ; Cell Survival ; Cell viability ; Collagen ; Degeneration ; Enzymes ; Estrogen ; Estrogens ; Estrogens - metabolism ; Estrogens - pharmacology ; Extracellular matrix ; Female ; Females ; Gene expression ; Health aspects ; Humans ; Interleukin 6 ; Interleukin-6 - metabolism ; Intervertebral Disc - metabolism ; Intervertebral Disc Degeneration - metabolism ; Intervertebral discs ; Matrix metalloproteinase ; Matrix metalloproteinases ; Metabolism ; Metalloproteinase ; Organ culture ; Organ Culture Techniques ; Osteoporosis ; Physiological aspects ; Post-menopause ; Postmenopausal women ; Vibration</subject><ispartof>International journal of molecular sciences, 2023-03, Vol.24 (7), p.6143</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c437t-5522a461aba754ffb381e5b67d851eabefaab13634546c8bd3e97c03470e67693</cites><orcidid>0000-0002-4782-1979 ; 0000-0003-4206-6326 ; 0000-0002-3333-2613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094023/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094023/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37047116$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Widmayer, Franziska</creatorcontrib><creatorcontrib>Neidlinger-Wilke, Cornelia</creatorcontrib><creatorcontrib>Witz, Fiona</creatorcontrib><creatorcontrib>Jansen, Jan U</creatorcontrib><creatorcontrib>Ignatius, Anita</creatorcontrib><creatorcontrib>Haffner-Luntzer, Melanie</creatorcontrib><creatorcontrib>Teixeira, Graciosa Q</creatorcontrib><title>Oestrogen and Vibration Improve Intervertebral Disc Cell Viability and Decrease Catabolism in Bovine Organ Cultures</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Postmenopausal women are at an increased risk for intervertebral disc degeneration, possibly due to the decrease in oestrogen levels. Low-magnitude, high-frequency vibration (LMHFV) is applied as a therapeutic approach for postmenopausal osteoporosis; however, less is known regarding possible effects on the intervertebral disc (IVD) and whether these may be oestrogen-dependent. The present study investigated the effect of 17β-oestradiol (E2) and LMHFV in an IVD organ culture model. Bovine IVDs (
= 6 IVDs/group) were treated with either (i) E2, (ii) LMHFV or (iii) the combination of E2 + LMHFV for 2 or 14 days. Minor changes in gene expression, cellularity and matrix metabolism were observed after E2 treatment, except for a significant increase in matrix metalloproteinase (MMP)-3 and interleukin (IL)-6 production. Interestingly, LMHFV alone induced cell loss and increased IL-6 production compared to the control. The combination of E2 + LMHFV induced a protective effect against cell loss and decreased IL-6 production compared to the LMHFV group. This indicates possible benefits of oestrogen therapy for the IVDs of postmenopausal women undergoing LMHFV exercises.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Back pain</subject><subject>Catabolism</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Collagen</subject><subject>Degeneration</subject><subject>Enzymes</subject><subject>Estrogen</subject><subject>Estrogens</subject><subject>Estrogens - metabolism</subject><subject>Estrogens - pharmacology</subject><subject>Extracellular matrix</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Interleukin 6</subject><subject>Interleukin-6 - metabolism</subject><subject>Intervertebral Disc - metabolism</subject><subject>Intervertebral Disc Degeneration - metabolism</subject><subject>Intervertebral discs</subject><subject>Matrix metalloproteinase</subject><subject>Matrix metalloproteinases</subject><subject>Metabolism</subject><subject>Metalloproteinase</subject><subject>Organ culture</subject><subject>Organ Culture Techniques</subject><subject>Osteoporosis</subject><subject>Physiological aspects</subject><subject>Post-menopause</subject><subject>Postmenopausal women</subject><subject>Vibration</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptks1vEzEQxS0EoqVw44wsceFAWn87PqGyhRKpUi6Fq2XvzgZHu3awdyP1v8elpU0R8sGW5zdv9J4GobeUnHJuyFnYjoUJohUV_Bk6poKxBSFKPz94H6FXpWwJYZxJ8xIdcU2EplQdo7KGMuW0gYhd7PCP4LObQop4Ne5y2gNexQnyHvIEtTLgi1Ba3MAwVNT5MITp5k_jBbQZXAHcuMn5NIQy4hDx57QPEfA6b1zEzTxMc4byGr3o3VDgzf19gr5__XLdfFtcrS9XzfnVohVcTwspGXNCUeedlqLvPV9SkF7pbikpOA-9c55yxYUUql36joPRLeFCE1BaGX6CPt3p7mY_QtdCnKoFu8thdPnGJhfs00oMP-0m7S0lxIgaVlX4cK-Q06-5JmXH6r-6dxHSXCxb1nSZoYxU9P0_6DbNOVZ_lmljlDRS00dq4wawIfapDm5vRe25ltQwTaWq1Ol_qHo6GEObIvSh_j9p-HjX0OZUSob-wSQl9nZL7OGWVPzdYTAP8N-14L8BYsi4Fg</recordid><startdate>20230324</startdate><enddate>20230324</enddate><creator>Widmayer, Franziska</creator><creator>Neidlinger-Wilke, Cornelia</creator><creator>Witz, Fiona</creator><creator>Jansen, Jan U</creator><creator>Ignatius, Anita</creator><creator>Haffner-Luntzer, Melanie</creator><creator>Teixeira, Graciosa Q</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4782-1979</orcidid><orcidid>https://orcid.org/0000-0003-4206-6326</orcidid><orcidid>https://orcid.org/0000-0002-3333-2613</orcidid></search><sort><creationdate>20230324</creationdate><title>Oestrogen and Vibration Improve Intervertebral Disc Cell Viability and Decrease Catabolism in Bovine Organ Cultures</title><author>Widmayer, Franziska ; 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Low-magnitude, high-frequency vibration (LMHFV) is applied as a therapeutic approach for postmenopausal osteoporosis; however, less is known regarding possible effects on the intervertebral disc (IVD) and whether these may be oestrogen-dependent. The present study investigated the effect of 17β-oestradiol (E2) and LMHFV in an IVD organ culture model. Bovine IVDs (
= 6 IVDs/group) were treated with either (i) E2, (ii) LMHFV or (iii) the combination of E2 + LMHFV for 2 or 14 days. Minor changes in gene expression, cellularity and matrix metabolism were observed after E2 treatment, except for a significant increase in matrix metalloproteinase (MMP)-3 and interleukin (IL)-6 production. Interestingly, LMHFV alone induced cell loss and increased IL-6 production compared to the control. The combination of E2 + LMHFV induced a protective effect against cell loss and decreased IL-6 production compared to the LMHFV group. This indicates possible benefits of oestrogen therapy for the IVDs of postmenopausal women undergoing LMHFV exercises.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37047116</pmid><doi>10.3390/ijms24076143</doi><orcidid>https://orcid.org/0000-0002-4782-1979</orcidid><orcidid>https://orcid.org/0000-0003-4206-6326</orcidid><orcidid>https://orcid.org/0000-0002-3333-2613</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Back pain Catabolism Cattle Cell culture Cell Survival Cell viability Collagen Degeneration Enzymes Estrogen Estrogens Estrogens - metabolism Estrogens - pharmacology Extracellular matrix Female Females Gene expression Health aspects Humans Interleukin 6 Interleukin-6 - metabolism Intervertebral Disc - metabolism Intervertebral Disc Degeneration - metabolism Intervertebral discs Matrix metalloproteinase Matrix metalloproteinases Metabolism Metalloproteinase Organ culture Organ Culture Techniques Osteoporosis Physiological aspects Post-menopause Postmenopausal women Vibration |
| title | Oestrogen and Vibration Improve Intervertebral Disc Cell Viability and Decrease Catabolism in Bovine Organ Cultures |
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