Structural basis for intrinsic transcription termination
Efficient and accurate termination is required for gene transcription in all living organisms 1 , 2 . Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway 3 , 4 —called intrinsic termination transcription in bacte...
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| Veröffentlicht in: | Nature (London) 2023-01, Vol.613 (7945), p.783-789 |
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| creator | You, Linlin Omollo, Expery O. Yu, Chengzhi Mooney, Rachel A. Shi, Jing Shen, Liqiang Wu, Xiaoxian Wen, Aijia He, Dingwei Zeng, Yuan Feng, Yu Landick, Robert Zhang, Yu |
| description | Efficient and accurate termination is required for gene transcription in all living organisms
1
,
2
. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway
3
,
4
—called intrinsic termination transcription in bacteria—in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of
Escherichia coli
transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.
Structural studies of
Escherichia coli
transcription intrinsic termination complexes representing distinct intermediates using cryo-electron microscopy provide insights into the steps and mechanism of transcription termination. |
| doi_str_mv | 10.1038/s41586-022-05604-1 |
| format | Article |
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1
,
2
. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway
3
,
4
—called intrinsic termination transcription in bacteria—in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of
Escherichia coli
transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.
Structural studies of
Escherichia coli
transcription intrinsic termination complexes representing distinct intermediates using cryo-electron microscopy provide insights into the steps and mechanism of transcription termination.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/s41586-022-05604-1</identifier><identifier>PMID: 36631609</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 631/337/572 ; 631/45/535 ; 82/83 ; Bacteria ; Cryoelectron Microscopy ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - metabolism ; DNA, Bacterial - ultrastructure ; DNA-directed RNA polymerase ; DNA-Directed RNA Polymerases - chemistry ; DNA-Directed RNA Polymerases - metabolism ; DNA-Directed RNA Polymerases - ultrastructure ; E coli ; Electron microscopy ; Escherichia coli - chemistry ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli - ultrastructure ; Eukaryotes ; Gene sequencing ; Humanities and Social Sciences ; Macromolecules ; Microscopy ; multidisciplinary ; Nucleotides ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA, Bacterial - chemistry ; RNA, Bacterial - genetics ; RNA, Bacterial - metabolism ; RNA, Bacterial - ultrastructure ; Science ; Science (multidisciplinary) ; Terminator Regions, Genetic - genetics ; Transcription termination ; Transcription Termination, Genetic</subject><ispartof>Nature (London), 2023-01, Vol.613 (7945), p.783-789</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature Limited.</rights><rights>Copyright Nature Publishing Group Jan 26, 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-935d552e5dd857b222a121ca7ff4a72122b89c52d433d96d2950f3afe0e643e53</citedby><cites>FETCH-LOGICAL-c475t-935d552e5dd857b222a121ca7ff4a72122b89c52d433d96d2950f3afe0e643e53</cites><orcidid>0000-0002-2317-9416 ; 0000-0001-5949-3442 ; 0000-0002-1778-8389 ; 0000-0002-5042-0383 ; 0000-0003-4239-8320</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/s41586-022-05604-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/s41586-022-05604-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36631609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>You, Linlin</creatorcontrib><creatorcontrib>Omollo, Expery O.</creatorcontrib><creatorcontrib>Yu, Chengzhi</creatorcontrib><creatorcontrib>Mooney, Rachel A.</creatorcontrib><creatorcontrib>Shi, Jing</creatorcontrib><creatorcontrib>Shen, Liqiang</creatorcontrib><creatorcontrib>Wu, Xiaoxian</creatorcontrib><creatorcontrib>Wen, Aijia</creatorcontrib><creatorcontrib>He, Dingwei</creatorcontrib><creatorcontrib>Zeng, Yuan</creatorcontrib><creatorcontrib>Feng, Yu</creatorcontrib><creatorcontrib>Landick, Robert</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><title>Structural basis for intrinsic transcription termination</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Efficient and accurate termination is required for gene transcription in all living organisms
1
,
2
. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway
3
,
4
—called intrinsic termination transcription in bacteria—in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of
Escherichia coli
transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.
Structural studies of
Escherichia coli
transcription intrinsic termination complexes representing distinct intermediates using cryo-electron microscopy provide insights into the steps and mechanism of transcription termination.</description><subject>101/28</subject><subject>631/337/572</subject><subject>631/45/535</subject><subject>82/83</subject><subject>Bacteria</subject><subject>Cryoelectron Microscopy</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Bacterial - ultrastructure</subject><subject>DNA-directed RNA polymerase</subject><subject>DNA-Directed RNA Polymerases - chemistry</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>DNA-Directed RNA Polymerases - ultrastructure</subject><subject>E coli</subject><subject>Electron microscopy</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli - ultrastructure</subject><subject>Eukaryotes</subject><subject>Gene sequencing</subject><subject>Humanities and Social Sciences</subject><subject>Macromolecules</subject><subject>Microscopy</subject><subject>multidisciplinary</subject><subject>Nucleotides</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Bacterial - metabolism</subject><subject>RNA, Bacterial - ultrastructure</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Terminator Regions, Genetic - genetics</subject><subject>Transcription termination</subject><subject>Transcription Termination, 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termination is required for gene transcription in all living organisms
1
,
2
. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway
3
,
4
—called intrinsic termination transcription in bacteria—in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of
Escherichia coli
transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.
Structural studies of
Escherichia coli
transcription intrinsic termination complexes representing distinct intermediates using cryo-electron microscopy provide insights into the steps and mechanism of transcription termination.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>36631609</pmid><doi>10.1038/s41586-022-05604-1</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-2317-9416</orcidid><orcidid>https://orcid.org/0000-0001-5949-3442</orcidid><orcidid>https://orcid.org/0000-0002-1778-8389</orcidid><orcidid>https://orcid.org/0000-0002-5042-0383</orcidid><orcidid>https://orcid.org/0000-0003-4239-8320</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 2023-01, Vol.613 (7945), p.783-789 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10091898 |
| source | MEDLINE; Nature; SpringerLink Journals - AutoHoldings |
| subjects | 101/28 631/337/572 631/45/535 82/83 Bacteria Cryoelectron Microscopy Deoxyribonucleic acid DNA DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - metabolism DNA, Bacterial - ultrastructure DNA-directed RNA polymerase DNA-Directed RNA Polymerases - chemistry DNA-Directed RNA Polymerases - metabolism DNA-Directed RNA Polymerases - ultrastructure E coli Electron microscopy Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli - ultrastructure Eukaryotes Gene sequencing Humanities and Social Sciences Macromolecules Microscopy multidisciplinary Nucleotides Ribonucleic acid RNA RNA polymerase RNA, Bacterial - chemistry RNA, Bacterial - genetics RNA, Bacterial - metabolism RNA, Bacterial - ultrastructure Science Science (multidisciplinary) Terminator Regions, Genetic - genetics Transcription termination Transcription Termination, Genetic |
| title | Structural basis for intrinsic transcription termination |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T04%3A38%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Structural%20basis%20for%20intrinsic%20transcription%20termination&rft.jtitle=Nature%20(London)&rft.au=You,%20Linlin&rft.date=2023-01-26&rft.volume=613&rft.issue=7945&rft.spage=783&rft.epage=789&rft.pages=783-789&rft.issn=0028-0836&rft.eissn=1476-4687&rft_id=info:doi/10.1038/s41586-022-05604-1&rft_dat=%3Cproquest_pubme%3E2765070209%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2770363848&rft_id=info:pmid/36631609&rfr_iscdi=true |