Rapid and precise genotyping of transgene zygosity in mice using an allele-specific method

Precise determination of transgene zygosity is essential for use of transgenic mice in research. Because integration loci of transgenes are usually unknown due to their random insertion, assessment of transgene zygosity remains a challenge. Current zygosity genotyping methods (progeny testing, qPCR,...

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Veröffentlicht in:	Life science alliance 2023-06, Vol.6 (6), p.e202201729
Hauptverfasser:	Yang, Jianqi, DeVore, Alison N, Fu, Daniel A, Spicer, Mackenzie M, Guo, Mengcheng, Thompson, Samantha G, Ahlers-Dannen, Katelin E, Polato, Federica, Nussenzweig, Andre, Fisher, Rory A
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Animals Base Sequence Genotype Methods Mice Mice, Transgenic Transgenes - genetics
Online-Zugang:	Volltext
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