Establishment of guinea pig kidney cell lines with potential application in the production of a classical swine fever live GPE− vaccine
Classical swine fever (CSF) live vaccine used in Japan, GPE− strain, is produced using guinea pig kidney (GPK)-derived primary culture cells. This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produce the CSF live vaccine, and alternative solution is d...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2023, Vol.85(3), pp.308-317 |
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| creator | SHIODA, Moe SHIOKAWA, Mai AOKI, Hiroshi |
| description | Classical swine fever (CSF) live vaccine used in Japan, GPE− strain, is produced using guinea pig kidney (GPK)-derived primary culture cells. This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produce the CSF live vaccine, and alternative solution is desired. Hence, we established two GPK cell lines capable of culturing the GPE− strain: spontaneously immortalized GPK (GPK-SI) cells were generated by repeated passaging of primary GPK cells, and the other cell line, artificially immortalized GPK (GPK-AI) cells, were obtained by introducing the SV40 large T antigen gene into primary GPK cells. Both cell lines were susceptible to the GPE− virus, and the virus grew more efficiently in GPK-SI cells at 37°C. When the culture temperature was set to 30°C, the virus titer reached 104.8 50% Tissue Culture Infectious Dose (TCID50)/mL in GPK-SI cells 7 days after virus inoculation at a multiplicity of infection (MOI) of 1, which was equivalent to that in cells cultured at 37°C. When the virus was inoculated at MOI |
| doi_str_mv | 10.1292/jvms.22-0385 |
| format | Article |
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This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produce the CSF live vaccine, and alternative solution is desired. Hence, we established two GPK cell lines capable of culturing the GPE− strain: spontaneously immortalized GPK (GPK-SI) cells were generated by repeated passaging of primary GPK cells, and the other cell line, artificially immortalized GPK (GPK-AI) cells, were obtained by introducing the SV40 large T antigen gene into primary GPK cells. Both cell lines were susceptible to the GPE− virus, and the virus grew more efficiently in GPK-SI cells at 37°C. When the culture temperature was set to 30°C, the virus titer reached 104.8 50% Tissue Culture Infectious Dose (TCID50)/mL in GPK-SI cells 7 days after virus inoculation at a multiplicity of infection (MOI) of 1, which was equivalent to that in cells cultured at 37°C. When the virus was inoculated at MOI <1, the virus titer 7 days after inoculation was higher when cultured at 30°C than when cultured at 37°C in both cell lines, reaching 105.63 TCID50/mL in GPK-SI cells. These results indicate that GPK-SI and GPK-AI cells can potentially replace primary GPK cells for the production of CSF live vaccines. This could also contribute to stable CSF vaccine production and animal welfare.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.22-0385</identifier><identifier>PMID: 36631081</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animal welfare ; Animals ; Antibodies, Viral ; Antigen T (large) ; Cell culture ; Cell Line ; Cell lines ; Classical Swine Fever ; Classical Swine Fever Virus - genetics ; Fever ; guinea pig kidney cells ; Guinea Pigs ; Hog cholera ; Immunization ; Inoculation ; Kidney ; Kidneys ; live vaccine ; Multiplicity of infection ; Swine ; Swine Diseases ; Tissue culture ; vaccine production ; Vaccines ; Vaccines, Attenuated ; Viral Vaccines ; Virology ; Viruses</subject><ispartof>Journal of Veterinary Medical Science, 2023, Vol.85(3), pp.308-317</ispartof><rights>2023 by the Japanese Society of Veterinary Science</rights><rights>2023. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). 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Vet. Med. Sci.</addtitle><description>Classical swine fever (CSF) live vaccine used in Japan, GPE− strain, is produced using guinea pig kidney (GPK)-derived primary culture cells. This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produce the CSF live vaccine, and alternative solution is desired. Hence, we established two GPK cell lines capable of culturing the GPE− strain: spontaneously immortalized GPK (GPK-SI) cells were generated by repeated passaging of primary GPK cells, and the other cell line, artificially immortalized GPK (GPK-AI) cells, were obtained by introducing the SV40 large T antigen gene into primary GPK cells. Both cell lines were susceptible to the GPE− virus, and the virus grew more efficiently in GPK-SI cells at 37°C. When the culture temperature was set to 30°C, the virus titer reached 104.8 50% Tissue Culture Infectious Dose (TCID50)/mL in GPK-SI cells 7 days after virus inoculation at a multiplicity of infection (MOI) of 1, which was equivalent to that in cells cultured at 37°C. When the virus was inoculated at MOI <1, the virus titer 7 days after inoculation was higher when cultured at 30°C than when cultured at 37°C in both cell lines, reaching 105.63 TCID50/mL in GPK-SI cells. These results indicate that GPK-SI and GPK-AI cells can potentially replace primary GPK cells for the production of CSF live vaccines. This could also contribute to stable CSF vaccine production and animal welfare.</description><subject>Animal welfare</subject><subject>Animals</subject><subject>Antibodies, Viral</subject><subject>Antigen T (large)</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Classical Swine Fever</subject><subject>Classical Swine Fever Virus - genetics</subject><subject>Fever</subject><subject>guinea pig kidney cells</subject><subject>Guinea Pigs</subject><subject>Hog cholera</subject><subject>Immunization</subject><subject>Inoculation</subject><subject>Kidney</subject><subject>Kidneys</subject><subject>live vaccine</subject><subject>Multiplicity of infection</subject><subject>Swine</subject><subject>Swine Diseases</subject><subject>Tissue culture</subject><subject>vaccine production</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated</subject><subject>Viral Vaccines</subject><subject>Virology</subject><subject>Viruses</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc9u1DAQhyMEokvhxhlZ4sKBFP-LHZ9QqZaCVAkOcLYc29l4ceJgJ6n6BnDlEXkSHHZZARePNPPpk2d-RfEUwQuEBX61X_p0gXEJSV3dKzaIUF5ySsT9YgMFYiXHFTwrHqW0hxAjysTD4owwRhCs0ab4vk2TarxLXW-HCYQW7GY3WAVGtwNfnBnsHdDWe-BzN4FbN3VgDFNmnfJAjaN3Wk0uDMANYOosGGMws_7dyTIFtFcpZcaDdJsVoLWLjdm2WHD9cfvz2w-wKK3z5HHxoFU-2SfHel58frv9dPWuvPlw_f7q8qbUlIqppAYybBgWFWmbqoLQakMrRnHNDUOaCdwaLbjlmgqmoeYmH4Y3DaaGamw0OS9eH7zj3PTW6LxKVF6O0fUq3smgnPx3MrhO7sIiEYScobrOhhdHQwxfZ5sm2bu0HkkNNsxJYs4qyAlhJKPP_0P3YY5D3k_iGiKEBIc0Uy8PlI4hpWjb028QlGvIcg1ZYizXkDP-7O8NTvCfVDPw5gDsc7g7ewJUnJz29mCrK0nW52g9DXWnorQD-QWBir5A</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>SHIODA, Moe</creator><creator>SHIOKAWA, Mai</creator><creator>AOKI, Hiroshi</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230301</creationdate><title>Establishment of guinea pig kidney cell lines with potential application in the production of a classical swine fever live GPE− vaccine</title><author>SHIODA, Moe ; SHIOKAWA, Mai ; AOKI, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-4d062d62953fb5500ecd4564287d61c692fdc97e7c496c0c7d0387bb24d4c2dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal welfare</topic><topic>Animals</topic><topic>Antibodies, Viral</topic><topic>Antigen T (large)</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Classical Swine Fever</topic><topic>Classical Swine Fever Virus - genetics</topic><topic>Fever</topic><topic>guinea pig kidney cells</topic><topic>Guinea Pigs</topic><topic>Hog cholera</topic><topic>Immunization</topic><topic>Inoculation</topic><topic>Kidney</topic><topic>Kidneys</topic><topic>live vaccine</topic><topic>Multiplicity of infection</topic><topic>Swine</topic><topic>Swine Diseases</topic><topic>Tissue culture</topic><topic>vaccine production</topic><topic>Vaccines</topic><topic>Vaccines, Attenuated</topic><topic>Viral Vaccines</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHIODA, Moe</creatorcontrib><creatorcontrib>SHIOKAWA, Mai</creatorcontrib><creatorcontrib>AOKI, Hiroshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHIODA, Moe</au><au>SHIOKAWA, Mai</au><au>AOKI, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of guinea pig kidney cell lines with potential application in the production of a classical swine fever live GPE− vaccine</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>85</volume><issue>3</issue><spage>308</spage><epage>317</epage><pages>308-317</pages><artnum>22-0385</artnum><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Classical swine fever (CSF) live vaccine used in Japan, GPE− strain, is produced using guinea pig kidney (GPK)-derived primary culture cells. This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produce the CSF live vaccine, and alternative solution is desired. Hence, we established two GPK cell lines capable of culturing the GPE− strain: spontaneously immortalized GPK (GPK-SI) cells were generated by repeated passaging of primary GPK cells, and the other cell line, artificially immortalized GPK (GPK-AI) cells, were obtained by introducing the SV40 large T antigen gene into primary GPK cells. Both cell lines were susceptible to the GPE− virus, and the virus grew more efficiently in GPK-SI cells at 37°C. When the culture temperature was set to 30°C, the virus titer reached 104.8 50% Tissue Culture Infectious Dose (TCID50)/mL in GPK-SI cells 7 days after virus inoculation at a multiplicity of infection (MOI) of 1, which was equivalent to that in cells cultured at 37°C. When the virus was inoculated at MOI <1, the virus titer 7 days after inoculation was higher when cultured at 30°C than when cultured at 37°C in both cell lines, reaching 105.63 TCID50/mL in GPK-SI cells. These results indicate that GPK-SI and GPK-AI cells can potentially replace primary GPK cells for the production of CSF live vaccines. This could also contribute to stable CSF vaccine production and animal welfare.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>36631081</pmid><doi>10.1292/jvms.22-0385</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal welfare Animals Antibodies, Viral Antigen T (large) Cell culture Cell Line Cell lines Classical Swine Fever Classical Swine Fever Virus - genetics Fever guinea pig kidney cells Guinea Pigs Hog cholera Immunization Inoculation Kidney Kidneys live vaccine Multiplicity of infection Swine Swine Diseases Tissue culture vaccine production Vaccines Vaccines, Attenuated Viral Vaccines Virology Viruses |
| title | Establishment of guinea pig kidney cell lines with potential application in the production of a classical swine fever live GPE− vaccine |
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