Screening of Differentially Expressed Genes Based on the ACRG Molecular Subtypes of Gastric Cancer and the Significance and Mechanism of AGTR1 Gene Expression
The Asian Cancer Research Group (ACRG) classification is a molecular classification established based on the tissues of gastric cancer (GC) patients in Asia. Patients with different ACRG subtypes differ significantly with regard to treatment response and prognosis, which indicates that the ACRG mole...
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| Veröffentlicht in: | Journal of personalized medicine 2023-03, Vol.13 (3), p.560 |
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| creator | Zhang, Haoran Zhen, Shuman Ding, Pingan Tan, Bibo Wang, Hongyan Liu, Wenbo Tian, Yuan Zhao, Qun |
| description | The Asian Cancer Research Group (ACRG) classification is a molecular classification established based on the tissues of gastric cancer (GC) patients in Asia. Patients with different ACRG subtypes differ significantly with regard to treatment response and prognosis, which indicates that the ACRG molecular classification is more valuable than the traditional pathological classification. However, the specific differentially expressed genes (DEGs) and the value of the ACRG molecular subtypes of GC have not been studied in depth.
Through the analysis of the GEO database, the DEGs in GC tissues of different ACRG molecular subtypes were investigated. The expression and mechanism of the screened angiotensin II receptor type 1 (
) gene were bioinformatically analyzed and experimentally verified. The role of
in GC cells was mainly investigated using CCK-8, wound-healing, transwell invasion assays, qRT-PCR, and Western blotting.
The bioinformatics results showed the presence of multiple DEGs in GC tissues with different ACRG molecular subtypes. Certain DEGs in GC tissues of different ACRG molecular subtypes have prognostic significance. AGTR1 levels in tumor tissues were significantly higher than in paired paracancerous tissues. The prognosis of GC patients with high expression of
was poor (
< 0.05). The
gene in GC samples was associated with the expression of immune pathways and immune checkpoint genes. After modifying
expression in cell lines, cells' proliferation, invasion, and migration abilities and the expression of related genes changed.
There were significant DEGs in GC tissues with different ACGR molecular types, among which the increased expression of
was a molecular feature of MSS/EMT type gastric cancer. Further study found that
was closely related to tumor immune infiltration and invasion and may be a new therapeutic target gene for gastric cancer. |
| doi_str_mv | 10.3390/jpm13030560 |
| format | Article |
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Through the analysis of the GEO database, the DEGs in GC tissues of different ACRG molecular subtypes were investigated. The expression and mechanism of the screened angiotensin II receptor type 1 (
) gene were bioinformatically analyzed and experimentally verified. The role of
in GC cells was mainly investigated using CCK-8, wound-healing, transwell invasion assays, qRT-PCR, and Western blotting.
The bioinformatics results showed the presence of multiple DEGs in GC tissues with different ACRG molecular subtypes. Certain DEGs in GC tissues of different ACRG molecular subtypes have prognostic significance. AGTR1 levels in tumor tissues were significantly higher than in paired paracancerous tissues. The prognosis of GC patients with high expression of
was poor (
< 0.05). The
gene in GC samples was associated with the expression of immune pathways and immune checkpoint genes. After modifying
expression in cell lines, cells' proliferation, invasion, and migration abilities and the expression of related genes changed.
There were significant DEGs in GC tissues with different ACGR molecular types, among which the increased expression of
was a molecular feature of MSS/EMT type gastric cancer. Further study found that
was closely related to tumor immune infiltration and invasion and may be a new therapeutic target gene for gastric cancer.</description><identifier>ISSN: 2075-4426</identifier><identifier>EISSN: 2075-4426</identifier><identifier>DOI: 10.3390/jpm13030560</identifier><identifier>PMID: 36983741</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Angiotensin ; Angiotensin II ; Angiotensin II receptors ; Bioinformatics ; Cell migration ; Cell proliferation ; Cholecystokinin ; Correlation analysis ; Gastric cancer ; Gene expression ; Immune checkpoint ; Medical diagnosis ; Medical prognosis ; Medical research ; Metastases ; Precision medicine ; Prognosis ; Proteins ; Reagents ; Survival analysis ; Therapeutic targets ; Tumors ; Western blotting ; Wound healing</subject><ispartof>Journal of personalized medicine, 2023-03, Vol.13 (3), p.560</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-37c0596f37c712d1c0b47f836e9048ae2e099d4d814d93faa8a8c344401acc573</citedby><cites>FETCH-LOGICAL-c410t-37c0596f37c712d1c0b47f836e9048ae2e099d4d814d93faa8a8c344401acc573</cites><orcidid>0009-0000-3004-896X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055834/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055834/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36983741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Haoran</creatorcontrib><creatorcontrib>Zhen, Shuman</creatorcontrib><creatorcontrib>Ding, Pingan</creatorcontrib><creatorcontrib>Tan, Bibo</creatorcontrib><creatorcontrib>Wang, Hongyan</creatorcontrib><creatorcontrib>Liu, Wenbo</creatorcontrib><creatorcontrib>Tian, Yuan</creatorcontrib><creatorcontrib>Zhao, Qun</creatorcontrib><title>Screening of Differentially Expressed Genes Based on the ACRG Molecular Subtypes of Gastric Cancer and the Significance and Mechanism of AGTR1 Gene Expression</title><title>Journal of personalized medicine</title><addtitle>J Pers Med</addtitle><description>The Asian Cancer Research Group (ACRG) classification is a molecular classification established based on the tissues of gastric cancer (GC) patients in Asia. Patients with different ACRG subtypes differ significantly with regard to treatment response and prognosis, which indicates that the ACRG molecular classification is more valuable than the traditional pathological classification. However, the specific differentially expressed genes (DEGs) and the value of the ACRG molecular subtypes of GC have not been studied in depth.
Through the analysis of the GEO database, the DEGs in GC tissues of different ACRG molecular subtypes were investigated. The expression and mechanism of the screened angiotensin II receptor type 1 (
) gene were bioinformatically analyzed and experimentally verified. The role of
in GC cells was mainly investigated using CCK-8, wound-healing, transwell invasion assays, qRT-PCR, and Western blotting.
The bioinformatics results showed the presence of multiple DEGs in GC tissues with different ACRG molecular subtypes. Certain DEGs in GC tissues of different ACRG molecular subtypes have prognostic significance. AGTR1 levels in tumor tissues were significantly higher than in paired paracancerous tissues. The prognosis of GC patients with high expression of
was poor (
< 0.05). The
gene in GC samples was associated with the expression of immune pathways and immune checkpoint genes. After modifying
expression in cell lines, cells' proliferation, invasion, and migration abilities and the expression of related genes changed.
There were significant DEGs in GC tissues with different ACGR molecular types, among which the increased expression of
was a molecular feature of MSS/EMT type gastric cancer. Further study found that
was closely related to tumor immune infiltration and invasion and may be a new therapeutic target gene for gastric cancer.</description><subject>Angiotensin</subject><subject>Angiotensin II</subject><subject>Angiotensin II receptors</subject><subject>Bioinformatics</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Cholecystokinin</subject><subject>Correlation analysis</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Immune checkpoint</subject><subject>Medical diagnosis</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Metastases</subject><subject>Precision medicine</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>Reagents</subject><subject>Survival analysis</subject><subject>Therapeutic targets</subject><subject>Tumors</subject><subject>Western blotting</subject><subject>Wound healing</subject><issn>2075-4426</issn><issn>2075-4426</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1rGzEQhkVpaEKaU-9F0EuhuJVW0n6ciuOm20JCIE7PQtaObJldaSvtlvrP5LdG63zgRJcZZp55Z0aD0AdKvjJWkW_bvqOMMCJy8gadZKQQM86z_O2Bf4zOYtyS9EqRZTl5h45ZXpWs4PQE3S11AHDWrbE3-Ic1BgK4waq23eGL_32AGKHBNTiI-FxNvnd42ACeL25qfOVb0GOrAl6Oq2HXJyjJ1CoOwWq8UE5DwMo1-4qlXTtrrJ6i--AV6I1yNnZT0by-vaH7Rk99rXfv0ZFRbYSzR3uK_vy8uF38ml1e178X88uZ5pQMM1ZoIqrcJFvQrKGarHhhSpZDRXipIANSVQ1vSsqbihmlSlVqxjknVGktCnaKvj_o9uOqg0anLwiqlX2wnQo76ZWVLzPObuTa_5OUECFKxpPC50eF4P-OEAfZ2aihbZUDP0aZFVUmCOW5SOinV-jWj8Gl_SaK5gnh00hfHigdfIwBzPM0lMjp9vLg9on-eLjAM_t0aXYPJkaqZA</recordid><startdate>20230320</startdate><enddate>20230320</enddate><creator>Zhang, Haoran</creator><creator>Zhen, Shuman</creator><creator>Ding, Pingan</creator><creator>Tan, Bibo</creator><creator>Wang, Hongyan</creator><creator>Liu, Wenbo</creator><creator>Tian, Yuan</creator><creator>Zhao, Qun</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0009-0000-3004-896X</orcidid></search><sort><creationdate>20230320</creationdate><title>Screening of Differentially Expressed Genes Based on the ACRG Molecular Subtypes of Gastric Cancer and the Significance and Mechanism of AGTR1 Gene Expression</title><author>Zhang, Haoran ; Zhen, Shuman ; Ding, Pingan ; Tan, Bibo ; Wang, Hongyan ; Liu, Wenbo ; Tian, Yuan ; Zhao, Qun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-37c0596f37c712d1c0b47f836e9048ae2e099d4d814d93faa8a8c344401acc573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Angiotensin</topic><topic>Angiotensin II</topic><topic>Angiotensin II receptors</topic><topic>Bioinformatics</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Cholecystokinin</topic><topic>Correlation analysis</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Immune checkpoint</topic><topic>Medical diagnosis</topic><topic>Medical prognosis</topic><topic>Medical research</topic><topic>Metastases</topic><topic>Precision medicine</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>Reagents</topic><topic>Survival analysis</topic><topic>Therapeutic targets</topic><topic>Tumors</topic><topic>Western blotting</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Haoran</creatorcontrib><creatorcontrib>Zhen, Shuman</creatorcontrib><creatorcontrib>Ding, Pingan</creatorcontrib><creatorcontrib>Tan, Bibo</creatorcontrib><creatorcontrib>Wang, Hongyan</creatorcontrib><creatorcontrib>Liu, Wenbo</creatorcontrib><creatorcontrib>Tian, Yuan</creatorcontrib><creatorcontrib>Zhao, Qun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of personalized medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Haoran</au><au>Zhen, Shuman</au><au>Ding, Pingan</au><au>Tan, Bibo</au><au>Wang, Hongyan</au><au>Liu, Wenbo</au><au>Tian, Yuan</au><au>Zhao, Qun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of Differentially Expressed Genes Based on the ACRG Molecular Subtypes of Gastric Cancer and the Significance and Mechanism of AGTR1 Gene Expression</atitle><jtitle>Journal of personalized medicine</jtitle><addtitle>J Pers Med</addtitle><date>2023-03-20</date><risdate>2023</risdate><volume>13</volume><issue>3</issue><spage>560</spage><pages>560-</pages><issn>2075-4426</issn><eissn>2075-4426</eissn><abstract>The Asian Cancer Research Group (ACRG) classification is a molecular classification established based on the tissues of gastric cancer (GC) patients in Asia. Patients with different ACRG subtypes differ significantly with regard to treatment response and prognosis, which indicates that the ACRG molecular classification is more valuable than the traditional pathological classification. However, the specific differentially expressed genes (DEGs) and the value of the ACRG molecular subtypes of GC have not been studied in depth.
Through the analysis of the GEO database, the DEGs in GC tissues of different ACRG molecular subtypes were investigated. The expression and mechanism of the screened angiotensin II receptor type 1 (
) gene were bioinformatically analyzed and experimentally verified. The role of
in GC cells was mainly investigated using CCK-8, wound-healing, transwell invasion assays, qRT-PCR, and Western blotting.
The bioinformatics results showed the presence of multiple DEGs in GC tissues with different ACRG molecular subtypes. Certain DEGs in GC tissues of different ACRG molecular subtypes have prognostic significance. AGTR1 levels in tumor tissues were significantly higher than in paired paracancerous tissues. The prognosis of GC patients with high expression of
was poor (
< 0.05). The
gene in GC samples was associated with the expression of immune pathways and immune checkpoint genes. After modifying
expression in cell lines, cells' proliferation, invasion, and migration abilities and the expression of related genes changed.
There were significant DEGs in GC tissues with different ACGR molecular types, among which the increased expression of
was a molecular feature of MSS/EMT type gastric cancer. Further study found that
was closely related to tumor immune infiltration and invasion and may be a new therapeutic target gene for gastric cancer.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36983741</pmid><doi>10.3390/jpm13030560</doi><orcidid>https://orcid.org/0009-0000-3004-896X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Angiotensin Angiotensin II Angiotensin II receptors Bioinformatics Cell migration Cell proliferation Cholecystokinin Correlation analysis Gastric cancer Gene expression Immune checkpoint Medical diagnosis Medical prognosis Medical research Metastases Precision medicine Prognosis Proteins Reagents Survival analysis Therapeutic targets Tumors Western blotting Wound healing |
| title | Screening of Differentially Expressed Genes Based on the ACRG Molecular Subtypes of Gastric Cancer and the Significance and Mechanism of AGTR1 Gene Expression |
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