Detection of enterovirus specific RNA sequences in muscle biopsy specimens from patients with adult onset myositis
A subgenomic cDNA probe with broad specificity for a range of enteroviruses was used to test by an in situ hybridisation technique for the presence of enterovirus specific genomic sequences in muscle biopsy samples obtained from patients with chronic adult myositis. Virus specific RNA sequences were...
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| Veröffentlicht in: | Annals of the rheumatic diseases 1990-05, Vol.49 (5), p.310-315 |
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| container_title | Annals of the rheumatic diseases |
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| creator | Yousef, G E Isenberg, D A Mowbray, J F |
| description | A subgenomic cDNA probe with broad specificity for a range of enteroviruses was used to test by an in situ hybridisation technique for the presence of enterovirus specific genomic sequences in muscle biopsy samples obtained from patients with chronic adult myositis. Virus specific RNA sequences were detected in 6/13 (46%) patients with idiopathic polymyositis or dermatomyositis. Control samples obtained from an equal number of patients suffering from other muscle disorders were negative. A monoclonal antibody specific for an enterovirus group was used to probe for viral antigens by indirect immunoperoxidase staining; all biopsy samples from test and control groups were negative. |
| doi_str_mv | 10.1136/ard.49.5.310 |
| format | Article |
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Virus specific RNA sequences were detected in 6/13 (46%) patients with idiopathic polymyositis or dermatomyositis. Control samples obtained from an equal number of patients suffering from other muscle disorders were negative. A monoclonal antibody specific for an enterovirus group was used to probe for viral antigens by indirect immunoperoxidase staining; all biopsy samples from test and control groups were negative.</description><identifier>ISSN: 0003-4967</identifier><identifier>EISSN: 1468-2060</identifier><identifier>DOI: 10.1136/ard.49.5.310</identifier><identifier>PMID: 2160802</identifier><identifier>CODEN: ARDIAO</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and European League Against Rheumatism</publisher><subject>Adult ; Biological and medical sciences ; DNA Probes ; Enterovirus - genetics ; Humans ; Immunoenzyme Techniques ; Medical sciences ; Molecular Probe Techniques ; Muscles - analysis ; Muscles - pathology ; myositis ; Myositis - metabolism ; Myositis - pathology ; RNA ; RNA, Viral - analysis ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><ispartof>Annals of the rheumatic diseases, 1990-05, Vol.49 (5), p.310-315</ispartof><rights>1991 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD May 1990</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b538t-75c453b9b7d4a965147cfe7ff3bc3be4ee46e6f71f41015ef9dc0fc5e3171fd53</citedby><cites>FETCH-LOGICAL-b538t-75c453b9b7d4a965147cfe7ff3bc3be4ee46e6f71f41015ef9dc0fc5e3171fd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1004075/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1004075/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19362342$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2160802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yousef, G E</creatorcontrib><creatorcontrib>Isenberg, D A</creatorcontrib><creatorcontrib>Mowbray, J F</creatorcontrib><title>Detection of enterovirus specific RNA sequences in muscle biopsy specimens from patients with adult onset myositis</title><title>Annals of the rheumatic diseases</title><addtitle>Ann Rheum Dis</addtitle><description>A subgenomic cDNA probe with broad specificity for a range of enteroviruses was used to test by an in situ hybridisation technique for the presence of enterovirus specific genomic sequences in muscle biopsy samples obtained from patients with chronic adult myositis. Virus specific RNA sequences were detected in 6/13 (46%) patients with idiopathic polymyositis or dermatomyositis. Control samples obtained from an equal number of patients suffering from other muscle disorders were negative. A monoclonal antibody specific for an enterovirus group was used to probe for viral antigens by indirect immunoperoxidase staining; all biopsy samples from test and control groups were negative.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>DNA Probes</subject><subject>Enterovirus - genetics</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Medical sciences</subject><subject>Molecular Probe Techniques</subject><subject>Muscles - analysis</subject><subject>Muscles - pathology</subject><subject>myositis</subject><subject>Myositis - metabolism</subject><subject>Myositis - pathology</subject><subject>RNA</subject><subject>RNA, Viral - analysis</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. 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Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. 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| ispartof | Annals of the rheumatic diseases, 1990-05, Vol.49 (5), p.310-315 |
| issn | 0003-4967 1468-2060 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1004075 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences DNA Probes Enterovirus - genetics Humans Immunoenzyme Techniques Medical sciences Molecular Probe Techniques Muscles - analysis Muscles - pathology myositis Myositis - metabolism Myositis - pathology RNA RNA, Viral - analysis Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis |
| title | Detection of enterovirus specific RNA sequences in muscle biopsy specimens from patients with adult onset myositis |
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