High-throughput line-illumination Raman microscopy with multislit detection

Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We developed a multiline-illumination Raman microscope to achieve ul...

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Veröffentlicht in:Biomedical optics express 2023-03, Vol.14 (3), p.1015-1026
Hauptverfasser: Mochizuki, Kentaro, Kumamoto, Yasuaki, Maeda, Shunsuke, Tanuma, Masato, Kasai, Atsushi, Takemura, Masashi, Harada, Yoshinori, Hashimoto, Hitoshi, Tanaka, Hideo, Smith, Nicholas Isaac, Fujita, Katsumasa
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container_issue 3
container_start_page 1015
container_title Biomedical optics express
container_volume 14
creator Mochizuki, Kentaro
Kumamoto, Yasuaki
Maeda, Shunsuke
Tanuma, Masato
Kasai, Atsushi
Takemura, Masashi
Harada, Yoshinori
Hashimoto, Hitoshi
Tanaka, Hideo
Smith, Nicholas Isaac
Fujita, Katsumasa
description Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We developed a multiline-illumination Raman microscope to achieve ultrafast Raman spectral imaging. A spectrophotometer equipped with a periodic array of confocal slits detects Raman spectra from a sample irradiated by multiple line illuminations. A comb-like Raman hyperspectral image is formed on a two-dimensional detector in the spectrophotometer, and a hyperspectral Raman image is acquired by scanning the sample with multiline illumination array. By irradiating a sample with 21 simultaneous illumination lines, we achieved high-throughput Raman hyperspectral imaging of mouse brain tissue, acquiring 1108800 spectra in 11.4 min. We also measured mouse kidney and liver tissue as well as conducted label-free live-cell molecular imaging. The ultrafast Raman hyperspectral imaging enabled by the presented technique will expand the possible applications of Raman microscopy in biological and medical fields.
doi_str_mv 10.1364/BOE.480611
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title High-throughput line-illumination Raman microscopy with multislit detection
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