Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats ( Nyctalus noctula ) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this stu...
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| Veröffentlicht in: | Virus genes 2023-04, Vol.59 (2), p.323-332 |
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| creator | Dafalla, Maysaa Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan Heckel, Gerald Ulrich, Rainer G. |
| description | Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (
Nyctalus noctula
) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. |
| doi_str_mv | 10.1007/s11262-022-01952-2 |
| format | Article |
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Nyctalus noctula
) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-022-01952-2</identifier><identifier>PMID: 36542315</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Bats ; Biomedical and Life Sciences ; Biomedicine ; Brief Report ; Chiroptera ; Europe ; Genetic screening ; Hantavirus ; Hantavirus Infections - epidemiology ; Hantavirus Infections - veterinary ; Host specificity ; Medical Microbiology ; Nyctalus noctula ; Orthohantavirus - genetics ; Phylogeny ; Plant Sciences ; Polymerase chain reaction ; Reverse transcription ; RNA, Viral - genetics ; Virology</subject><ispartof>Virus genes, 2023-04, Vol.59 (2), p.323-332</ispartof><rights>The Author(s) 2022</rights><rights>2022. The Author(s).</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-fb6e51a7100e4e1c2b8ed75650564a8b3292fed5cc0a020e60359b6f917945cd3</citedby><cites>FETCH-LOGICAL-c475t-fb6e51a7100e4e1c2b8ed75650564a8b3292fed5cc0a020e60359b6f917945cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-022-01952-2$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-022-01952-2$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36542315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dafalla, Maysaa</creatorcontrib><creatorcontrib>Orłowska, Anna</creatorcontrib><creatorcontrib>Keleş, Sinan Julian</creatorcontrib><creatorcontrib>Straková, Petra</creatorcontrib><creatorcontrib>Schlottau, Kore</creatorcontrib><creatorcontrib>Jeske, Kathrin</creatorcontrib><creatorcontrib>Hoffmann, Bernd</creatorcontrib><creatorcontrib>Wibbelt, Gudrun</creatorcontrib><creatorcontrib>Smreczak, Marcin</creatorcontrib><creatorcontrib>Müller, Thomas</creatorcontrib><creatorcontrib>Freuling, Conrad Martin</creatorcontrib><creatorcontrib>Wang, Xuejing</creatorcontrib><creatorcontrib>Rola, Jerzy</creatorcontrib><creatorcontrib>Drewes, Stephan</creatorcontrib><creatorcontrib>Fereidouni, Sasan</creatorcontrib><creatorcontrib>Heckel, Gerald</creatorcontrib><creatorcontrib>Ulrich, Rainer G.</creatorcontrib><title>Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (
Nyctalus noctula
) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations.</description><subject>Animals</subject><subject>Bats</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brief Report</subject><subject>Chiroptera</subject><subject>Europe</subject><subject>Genetic screening</subject><subject>Hantavirus</subject><subject>Hantavirus Infections - epidemiology</subject><subject>Hantavirus Infections - veterinary</subject><subject>Host specificity</subject><subject>Medical Microbiology</subject><subject>Nyctalus noctula</subject><subject>Orthohantavirus - genetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Polymerase chain reaction</subject><subject>Reverse transcription</subject><subject>RNA, Viral - genetics</subject><subject>Virology</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctu1DAUhi0EotPCC7BAlti0i4Dt-BKvUBkVilTBBiR2luOczLhK7GAnRcPT45JSLgsWluXj7_zn8iP0jJKXlBD1KlPKJKsIK4dqwSr2AG2oUKzSmn95iDZEM1I1QuojdJzzNSGkaRh_jI5qKTirqdig75c2zPbGpyXjNylEPEQb1qfPeO93--GA8wTO997hOeJ5D9jFcYwBh-jmZQDc2hmffji42Q4lbY3aM2xDh7_5DvKUwHbYB7yFMCc74IslxQmeoEe9HTI8vbtP0Oe3F5-2l9XVx3fvt-dXleNKzFXfShDUqjIycKCOtQ10SkhBhOS2aWumWQ-dcI5YwghIUgvdyl5TpblwXX2CXq-609KO0Lm1CTMlP9p0MNF68_dP8HuzizemVGSCcVoUTu8UUvy6QJ7N6LODYbAB4pINK-1IRXjNCvriH_Q6LimU-QpVtq9oU8tCsZVyKeacoL_vhpLbssqs3prirfnprbmVfv7nHPcpv8wsQL0CZeM-7CD9rv0f2R8TNLDx</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Dafalla, Maysaa</creator><creator>Orłowska, Anna</creator><creator>Keleş, Sinan Julian</creator><creator>Straková, Petra</creator><creator>Schlottau, Kore</creator><creator>Jeske, Kathrin</creator><creator>Hoffmann, Bernd</creator><creator>Wibbelt, Gudrun</creator><creator>Smreczak, Marcin</creator><creator>Müller, Thomas</creator><creator>Freuling, Conrad Martin</creator><creator>Wang, Xuejing</creator><creator>Rola, Jerzy</creator><creator>Drewes, Stephan</creator><creator>Fereidouni, Sasan</creator><creator>Heckel, Gerald</creator><creator>Ulrich, Rainer G.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230401</creationdate><title>Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe</title><author>Dafalla, Maysaa ; Orłowska, Anna ; Keleş, Sinan Julian ; Straková, Petra ; Schlottau, Kore ; Jeske, Kathrin ; Hoffmann, Bernd ; Wibbelt, Gudrun ; Smreczak, Marcin ; Müller, Thomas ; Freuling, Conrad Martin ; Wang, Xuejing ; Rola, Jerzy ; Drewes, Stephan ; Fereidouni, Sasan ; Heckel, Gerald ; Ulrich, Rainer G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-fb6e51a7100e4e1c2b8ed75650564a8b3292fed5cc0a020e60359b6f917945cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bats</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brief Report</topic><topic>Chiroptera</topic><topic>Europe</topic><topic>Genetic screening</topic><topic>Hantavirus</topic><topic>Hantavirus Infections - epidemiology</topic><topic>Hantavirus Infections - veterinary</topic><topic>Host specificity</topic><topic>Medical Microbiology</topic><topic>Nyctalus noctula</topic><topic>Orthohantavirus - genetics</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Polymerase chain reaction</topic><topic>Reverse transcription</topic><topic>RNA, Viral - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dafalla, Maysaa</creatorcontrib><creatorcontrib>Orłowska, Anna</creatorcontrib><creatorcontrib>Keleş, Sinan Julian</creatorcontrib><creatorcontrib>Straková, Petra</creatorcontrib><creatorcontrib>Schlottau, Kore</creatorcontrib><creatorcontrib>Jeske, Kathrin</creatorcontrib><creatorcontrib>Hoffmann, Bernd</creatorcontrib><creatorcontrib>Wibbelt, Gudrun</creatorcontrib><creatorcontrib>Smreczak, Marcin</creatorcontrib><creatorcontrib>Müller, Thomas</creatorcontrib><creatorcontrib>Freuling, Conrad Martin</creatorcontrib><creatorcontrib>Wang, Xuejing</creatorcontrib><creatorcontrib>Rola, Jerzy</creatorcontrib><creatorcontrib>Drewes, Stephan</creatorcontrib><creatorcontrib>Fereidouni, Sasan</creatorcontrib><creatorcontrib>Heckel, Gerald</creatorcontrib><creatorcontrib>Ulrich, Rainer G.</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dafalla, Maysaa</au><au>Orłowska, Anna</au><au>Keleş, Sinan Julian</au><au>Straková, Petra</au><au>Schlottau, Kore</au><au>Jeske, Kathrin</au><au>Hoffmann, Bernd</au><au>Wibbelt, Gudrun</au><au>Smreczak, Marcin</au><au>Müller, Thomas</au><au>Freuling, Conrad Martin</au><au>Wang, Xuejing</au><au>Rola, Jerzy</au><au>Drewes, Stephan</au><au>Fereidouni, Sasan</au><au>Heckel, Gerald</au><au>Ulrich, Rainer G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2023-04-01</date><risdate>2023</risdate><volume>59</volume><issue>2</issue><spage>323</spage><epage>332</epage><pages>323-332</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (
Nyctalus noctula
) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>36542315</pmid><doi>10.1007/s11262-022-01952-2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virus genes, 2023-04, Vol.59 (2), p.323-332 |
| issn | 0920-8569 1572-994X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Bats Biomedical and Life Sciences Biomedicine Brief Report Chiroptera Europe Genetic screening Hantavirus Hantavirus Infections - epidemiology Hantavirus Infections - veterinary Host specificity Medical Microbiology Nyctalus noctula Orthohantavirus - genetics Phylogeny Plant Sciences Polymerase chain reaction Reverse transcription RNA, Viral - genetics Virology |
| title | Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
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