Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences
Background The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study aimed to determine the genetic diversity and relationships of several Indonesian local sheep populations on Java Island, Indonesia, based on the mtDNA...
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| description | Background
The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study aimed to determine the genetic diversity and relationships of several Indonesian local sheep populations on Java Island, Indonesia, based on the mtDNA Cyt b gene sequences. Blood samples were collected from forty-one individual sheep in seven populations of Indonesia local sheep breeds on Java Island (Priangan = 6, Garut = 6, Batur = 7, Wonosobo = 5, Javanese Thin-Tailed/JTT = 7, Javanese Fat-Tailed/JFT = 5, and Sapudi = 5). DNA extraction was performed on blood samples, and the mtDNA Cyt b gene was amplified using specific primers (Alek-CBF: 5'-CAACCCCACCACTTACAA-3' and Alek-CBR: 5'-CCTTGAGTCTTAGGGAGGTT-3'). The polymerase chain reaction (PCR) products were then sequenced, and data were analyzed using the MEGA version 7.0, DNA SP version 6.0, and NTSYS-pc version 2.11 software.
Results
A total of 1140 bp complete mtDNA Cyt b gene sequences in this study obtained 1134 monomorphic sites (I), six polymorphic sites (V), one segregation site (S), and five parsimony informative sites (P) with a nucleotide diversity (Pi), the average number of nucleotide differences (K), and sequence conservation (SC) were 0.00119, 1.35610, and 0.9947, respectively. There were six haplotypes consisting of two unique haplotypes and four shared haplotypes with a haplotype diversity (Hd) of 0.5415. The genetic distance within and between populations ranged from 0.0000 to 0.0016 and 0.0000 to 0.0020, respectively. Wonosobo, JFT, and Sapudi sheep have the closest relationship, and then these three breeds were close to JTT sheep, followed by Batur, Priangan, and Garut sheep. Two haplogroups have been found based on the Ovine haplogroup clustering. All Wonosobo, JTT, JFT, Sapudi sheep, and most Batur sheep were clustered into haplogroup B. In contrast, Garut sheep were mostly clustered into haplogroup A, while Priangan sheep were clustered into both haplogroups with the same percentage.
Conclusion
Seven Indonesian local sheep breeds on Java Island have a close relationship clustered into two haplogroups, namely haplogroups A and B. Most Indonesian local sheep breeds on Java Island in this study were clustered into haplogroup B, except for Garut and Priangan sheep. |
| doi_str_mv | 10.1186/s43141-023-00491-z |
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The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study aimed to determine the genetic diversity and relationships of several Indonesian local sheep populations on Java Island, Indonesia, based on the mtDNA Cyt b gene sequences. Blood samples were collected from forty-one individual sheep in seven populations of Indonesia local sheep breeds on Java Island (Priangan = 6, Garut = 6, Batur = 7, Wonosobo = 5, Javanese Thin-Tailed/JTT = 7, Javanese Fat-Tailed/JFT = 5, and Sapudi = 5). DNA extraction was performed on blood samples, and the mtDNA Cyt b gene was amplified using specific primers (Alek-CBF: 5'-CAACCCCACCACTTACAA-3' and Alek-CBR: 5'-CCTTGAGTCTTAGGGAGGTT-3'). The polymerase chain reaction (PCR) products were then sequenced, and data were analyzed using the MEGA version 7.0, DNA SP version 6.0, and NTSYS-pc version 2.11 software.
Results
A total of 1140 bp complete mtDNA Cyt b gene sequences in this study obtained 1134 monomorphic sites (I), six polymorphic sites (V), one segregation site (S), and five parsimony informative sites (P) with a nucleotide diversity (Pi), the average number of nucleotide differences (K), and sequence conservation (SC) were 0.00119, 1.35610, and 0.9947, respectively. There were six haplotypes consisting of two unique haplotypes and four shared haplotypes with a haplotype diversity (Hd) of 0.5415. The genetic distance within and between populations ranged from 0.0000 to 0.0016 and 0.0000 to 0.0020, respectively. Wonosobo, JFT, and Sapudi sheep have the closest relationship, and then these three breeds were close to JTT sheep, followed by Batur, Priangan, and Garut sheep. Two haplogroups have been found based on the Ovine haplogroup clustering. All Wonosobo, JTT, JFT, Sapudi sheep, and most Batur sheep were clustered into haplogroup B. In contrast, Garut sheep were mostly clustered into haplogroup A, while Priangan sheep were clustered into both haplogroups with the same percentage.
Conclusion
Seven Indonesian local sheep breeds on Java Island have a close relationship clustered into two haplogroups, namely haplogroups A and B. Most Indonesian local sheep breeds on Java Island in this study were clustered into haplogroup B, except for Garut and Priangan sheep.</description><identifier>ISSN: 1687-157X</identifier><identifier>EISSN: 2090-5920</identifier><identifier>DOI: 10.1186/s43141-023-00491-z</identifier><identifier>PMID: 36930372</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Bioinformatics ; Biological diversity ; Biomedical Engineering and Bioengineering ; Biotechnology ; Blood ; Clustering ; computer software ; Conserved sequence ; CytB gene ; Cytochrome ; Cytochrome b ; Cytochromes ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; Engineering ; Ethylenediaminetetraacetic acid ; Gene sequencing ; Genes ; Genetic distance ; Genetic diversity ; Genetic Engineering ; genetic profile ; Genetic variation ; Genetics and Genomics ; germplasm conservation ; Haplotypes ; Indonesia ; Indonesian local sheep ; Java (Computer program language) ; Laboratories ; Maternal origin ; mitochondria ; Mitochondrial DNA ; mtDNA Cyt b gene ; Nucleotides ; Ovis aries ; Phylogenetic ; Phylogenetics ; Polymerase chain reaction ; Populations ; Proteomics ; Sheep ; Software ; Veterinary medicine</subject><ispartof>Journal of Genetic Engineering and Biotechnology, 2023-12, Vol.21 (1), p.34-12, Article 34</ispartof><rights>The Author(s) 2023</rights><rights>2023. The Author(s).</rights><rights>COPYRIGHT 2023 Springer</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c613t-ce43059a6f82198ecdee318043731439da37ac63517ad26764f517705b7c03d23</citedby><cites>FETCH-LOGICAL-c613t-ce43059a6f82198ecdee318043731439da37ac63517ad26764f517705b7c03d23</cites><orcidid>0000-0003-1010-7429</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10023820/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10023820/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36930372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ibrahim, Alek</creatorcontrib><creatorcontrib>Baliarti, Endang</creatorcontrib><creatorcontrib>Budisatria, I Gede Suparta</creatorcontrib><creatorcontrib>Artama, Wayan Tunas</creatorcontrib><creatorcontrib>Widayanti, Rini</creatorcontrib><creatorcontrib>Maharani, Dyah</creatorcontrib><creatorcontrib>Tavares, Luis</creatorcontrib><creatorcontrib>Margawati, Endang Tri</creatorcontrib><title>Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences</title><title>Journal of Genetic Engineering and Biotechnology</title><addtitle>J Genet Eng Biotechnol</addtitle><addtitle>J Genet Eng Biotechnol</addtitle><description>Background
The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study aimed to determine the genetic diversity and relationships of several Indonesian local sheep populations on Java Island, Indonesia, based on the mtDNA Cyt b gene sequences. Blood samples were collected from forty-one individual sheep in seven populations of Indonesia local sheep breeds on Java Island (Priangan = 6, Garut = 6, Batur = 7, Wonosobo = 5, Javanese Thin-Tailed/JTT = 7, Javanese Fat-Tailed/JFT = 5, and Sapudi = 5). DNA extraction was performed on blood samples, and the mtDNA Cyt b gene was amplified using specific primers (Alek-CBF: 5'-CAACCCCACCACTTACAA-3' and Alek-CBR: 5'-CCTTGAGTCTTAGGGAGGTT-3'). The polymerase chain reaction (PCR) products were then sequenced, and data were analyzed using the MEGA version 7.0, DNA SP version 6.0, and NTSYS-pc version 2.11 software.
Results
A total of 1140 bp complete mtDNA Cyt b gene sequences in this study obtained 1134 monomorphic sites (I), six polymorphic sites (V), one segregation site (S), and five parsimony informative sites (P) with a nucleotide diversity (Pi), the average number of nucleotide differences (K), and sequence conservation (SC) were 0.00119, 1.35610, and 0.9947, respectively. There were six haplotypes consisting of two unique haplotypes and four shared haplotypes with a haplotype diversity (Hd) of 0.5415. The genetic distance within and between populations ranged from 0.0000 to 0.0016 and 0.0000 to 0.0020, respectively. Wonosobo, JFT, and Sapudi sheep have the closest relationship, and then these three breeds were close to JTT sheep, followed by Batur, Priangan, and Garut sheep. Two haplogroups have been found based on the Ovine haplogroup clustering. All Wonosobo, JTT, JFT, Sapudi sheep, and most Batur sheep were clustered into haplogroup B. In contrast, Garut sheep were mostly clustered into haplogroup A, while Priangan sheep were clustered into both haplogroups with the same percentage.
Conclusion
Seven Indonesian local sheep breeds on Java Island have a close relationship clustered into two haplogroups, namely haplogroups A and B. Most Indonesian local sheep breeds on Java Island in this study were clustered into haplogroup B, except for Garut and Priangan sheep.</description><subject>Analysis</subject><subject>Bioinformatics</subject><subject>Biological diversity</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biotechnology</subject><subject>Blood</subject><subject>Clustering</subject><subject>computer software</subject><subject>Conserved sequence</subject><subject>CytB gene</subject><subject>Cytochrome</subject><subject>Cytochrome b</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Engineering</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic distance</subject><subject>Genetic diversity</subject><subject>Genetic Engineering</subject><subject>genetic profile</subject><subject>Genetic variation</subject><subject>Genetics and Genomics</subject><subject>germplasm conservation</subject><subject>Haplotypes</subject><subject>Indonesia</subject><subject>Indonesian local sheep</subject><subject>Java (Computer program language)</subject><subject>Laboratories</subject><subject>Maternal origin</subject><subject>mitochondria</subject><subject>Mitochondrial DNA</subject><subject>mtDNA Cyt b gene</subject><subject>Nucleotides</subject><subject>Ovis aries</subject><subject>Phylogenetic</subject><subject>Phylogenetics</subject><subject>Polymerase chain reaction</subject><subject>Populations</subject><subject>Proteomics</subject><subject>Sheep</subject><subject>Software</subject><subject>Veterinary medicine</subject><issn>1687-157X</issn><issn>2090-5920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNp9UsFu1DAUjBCIVqU_wAFZ4sIlxY6d2DmhqiplUSUuIHGzHPsl61ViL3Z2pe0X8Nm8bUqhHEgOcZ5n5j2PpyheM3rBmGreZ8GZYCWteEmpaFl596w4rWhLy7qt6PPilDVKlqyW30-K85w3FJ9aKFazl8UJb1pOuaxOi583EGD2lji_h5T9fCAmOJJgNLOPIa_9lpgphoGsgosBsjeBjNGakeQ1wJZ0CcBlEgP5bPaGrPJ45HcmgzsWJz9Hu47BJY8Uezj-pTgB6ciAnUmGHzsIFvKr4kVvxgznD9-z4tvH669Xn8rbLzerq8vb0jaMz6UFwWndmqZXFWsVWAfAmaKCS_SDt85waWzDayaNqxrZiB6XktadtJS7ip8Vq0XXRbPR2-Qnkw46Gq_vCzEN2iQ0ZARNeyddIyTvay4YGKUM433bcW6wRWVR68Oitd11EzgLYU5mfCL6dCf4tR7iXjOK96YqigrvHhRSRCPyrCefLYxoIsRd1pzVnLVNIxRC3_4D3cRdCuiVrqSSUrYtrxF1saAGgyfwoY_Y2OLrYPIWL7D3WL-UAjPEhGqQUC0Em2LOCfrH8RnVx6TpJWkaJ9b3SdN3SHrz98EfKb9zhQC-ADJuhQHSn2H_I_sL9cPgMg</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Ibrahim, Alek</creator><creator>Baliarti, Endang</creator><creator>Budisatria, I Gede Suparta</creator><creator>Artama, Wayan Tunas</creator><creator>Widayanti, Rini</creator><creator>Maharani, Dyah</creator><creator>Tavares, Luis</creator><creator>Margawati, Endang Tri</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><general>Elsevier</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1010-7429</orcidid></search><sort><creationdate>20231201</creationdate><title>Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences</title><author>Ibrahim, Alek ; Baliarti, Endang ; Budisatria, I Gede Suparta ; Artama, Wayan Tunas ; Widayanti, Rini ; Maharani, Dyah ; Tavares, Luis ; Margawati, Endang Tri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c613t-ce43059a6f82198ecdee318043731439da37ac63517ad26764f517705b7c03d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Bioinformatics</topic><topic>Biological diversity</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biotechnology</topic><topic>Blood</topic><topic>Clustering</topic><topic>computer software</topic><topic>Conserved sequence</topic><topic>CytB gene</topic><topic>Cytochrome</topic><topic>Cytochrome b</topic><topic>Cytochromes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Engineering</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic distance</topic><topic>Genetic diversity</topic><topic>Genetic Engineering</topic><topic>genetic profile</topic><topic>Genetic variation</topic><topic>Genetics and Genomics</topic><topic>germplasm conservation</topic><topic>Haplotypes</topic><topic>Indonesia</topic><topic>Indonesian local sheep</topic><topic>Java (Computer program language)</topic><topic>Laboratories</topic><topic>Maternal origin</topic><topic>mitochondria</topic><topic>Mitochondrial DNA</topic><topic>mtDNA Cyt b gene</topic><topic>Nucleotides</topic><topic>Ovis aries</topic><topic>Phylogenetic</topic><topic>Phylogenetics</topic><topic>Polymerase chain reaction</topic><topic>Populations</topic><topic>Proteomics</topic><topic>Sheep</topic><topic>Software</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ibrahim, Alek</creatorcontrib><creatorcontrib>Baliarti, Endang</creatorcontrib><creatorcontrib>Budisatria, I Gede Suparta</creatorcontrib><creatorcontrib>Artama, Wayan Tunas</creatorcontrib><creatorcontrib>Widayanti, Rini</creatorcontrib><creatorcontrib>Maharani, Dyah</creatorcontrib><creatorcontrib>Tavares, Luis</creatorcontrib><creatorcontrib>Margawati, Endang Tri</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of Genetic Engineering and Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ibrahim, Alek</au><au>Baliarti, Endang</au><au>Budisatria, I Gede Suparta</au><au>Artama, Wayan Tunas</au><au>Widayanti, Rini</au><au>Maharani, Dyah</au><au>Tavares, Luis</au><au>Margawati, Endang Tri</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences</atitle><jtitle>Journal of Genetic Engineering and Biotechnology</jtitle><stitle>J Genet Eng Biotechnol</stitle><addtitle>J Genet Eng Biotechnol</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>21</volume><issue>1</issue><spage>34</spage><epage>12</epage><pages>34-12</pages><artnum>34</artnum><issn>1687-157X</issn><eissn>2090-5920</eissn><abstract>Background
The cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study aimed to determine the genetic diversity and relationships of several Indonesian local sheep populations on Java Island, Indonesia, based on the mtDNA Cyt b gene sequences. Blood samples were collected from forty-one individual sheep in seven populations of Indonesia local sheep breeds on Java Island (Priangan = 6, Garut = 6, Batur = 7, Wonosobo = 5, Javanese Thin-Tailed/JTT = 7, Javanese Fat-Tailed/JFT = 5, and Sapudi = 5). DNA extraction was performed on blood samples, and the mtDNA Cyt b gene was amplified using specific primers (Alek-CBF: 5'-CAACCCCACCACTTACAA-3' and Alek-CBR: 5'-CCTTGAGTCTTAGGGAGGTT-3'). The polymerase chain reaction (PCR) products were then sequenced, and data were analyzed using the MEGA version 7.0, DNA SP version 6.0, and NTSYS-pc version 2.11 software.
Results
A total of 1140 bp complete mtDNA Cyt b gene sequences in this study obtained 1134 monomorphic sites (I), six polymorphic sites (V), one segregation site (S), and five parsimony informative sites (P) with a nucleotide diversity (Pi), the average number of nucleotide differences (K), and sequence conservation (SC) were 0.00119, 1.35610, and 0.9947, respectively. There were six haplotypes consisting of two unique haplotypes and four shared haplotypes with a haplotype diversity (Hd) of 0.5415. The genetic distance within and between populations ranged from 0.0000 to 0.0016 and 0.0000 to 0.0020, respectively. Wonosobo, JFT, and Sapudi sheep have the closest relationship, and then these three breeds were close to JTT sheep, followed by Batur, Priangan, and Garut sheep. Two haplogroups have been found based on the Ovine haplogroup clustering. All Wonosobo, JTT, JFT, Sapudi sheep, and most Batur sheep were clustered into haplogroup B. In contrast, Garut sheep were mostly clustered into haplogroup A, while Priangan sheep were clustered into both haplogroups with the same percentage.
Conclusion
Seven Indonesian local sheep breeds on Java Island have a close relationship clustered into two haplogroups, namely haplogroups A and B. Most Indonesian local sheep breeds on Java Island in this study were clustered into haplogroup B, except for Garut and Priangan sheep.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36930372</pmid><doi>10.1186/s43141-023-00491-z</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1010-7429</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Genetic Engineering and Biotechnology, 2023-12, Vol.21 (1), p.34-12, Article 34 |
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Springer Nature OA Free Journals |
| subjects | Analysis Bioinformatics Biological diversity Biomedical Engineering and Bioengineering Biotechnology Blood Clustering computer software Conserved sequence CytB gene Cytochrome Cytochrome b Cytochromes Deoxyribonucleic acid DNA DNA sequencing Engineering Ethylenediaminetetraacetic acid Gene sequencing Genes Genetic distance Genetic diversity Genetic Engineering genetic profile Genetic variation Genetics and Genomics germplasm conservation Haplotypes Indonesia Indonesian local sheep Java (Computer program language) Laboratories Maternal origin mitochondria Mitochondrial DNA mtDNA Cyt b gene Nucleotides Ovis aries Phylogenetic Phylogenetics Polymerase chain reaction Populations Proteomics Sheep Software Veterinary medicine |
| title | Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T23%3A32%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20diversity%20and%20relationship%20among%20Indonesian%20local%20sheep%20breeds%20on%20Java%20Island%20based%20on%20mitochondrial%20cytochrome%20b%20gene%20sequences&rft.jtitle=Journal%20of%20Genetic%20Engineering%20and%20Biotechnology&rft.au=Ibrahim,%20Alek&rft.date=2023-12-01&rft.volume=21&rft.issue=1&rft.spage=34&rft.epage=12&rft.pages=34-12&rft.artnum=34&rft.issn=1687-157X&rft.eissn=2090-5920&rft_id=info:doi/10.1186/s43141-023-00491-z&rft_dat=%3Cgale_doaj_%3EA741861486%3C/gale_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2787779935&rft_id=info:pmid/36930372&rft_galeid=A741861486&rft_doaj_id=oai_doaj_org_article_0fd7d6473f5341ea88a13f9b33aac62c&rfr_iscdi=true |