Flow cytometric evaluation of the neutrophil compartment in COVID‐19 at hospital presentation: A normal response to an abnormal situation
Coronavirus disease 2019 (COVID‐19) is a rapidly emerging pandemic disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Critical COVID‐19 is thought to be associated with a hyper‐inflammatory process that can develop into acute respiratory distress syndrome, a critical...
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| Veröffentlicht in: | Journal of leukocyte biology 2021-01, Vol.109 (1), p.99-114 |
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| creator | Spijkerman, Roy Bongers, Suzanne H. Bindels, Bas J. J. Tinnevelt, Gerjen H. Giustarini, Giulio Jorritsma, Nikita K. N. Buitenwerf, Wiebe Spengler, Daan E. J. Delemarre, Eveline M. Nierkens, Stefan Goor, Harriët M. R. Jansen, Jeroen J. Vrisekoop, Nienke Hietbrink, Falco Leenen, Luke P. H. Kaasjager, Karin A. H. Koenderman, Leo |
| description | Coronavirus disease 2019 (COVID‐19) is a rapidly emerging pandemic disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Critical COVID‐19 is thought to be associated with a hyper‐inflammatory process that can develop into acute respiratory distress syndrome, a critical disease normally mediated by dysfunctional neutrophils. This study tested the hypothesis whether the neutrophil compartment displays characteristics of hyperinflammation in COVID‐19 patients. Therefore, a prospective study was performed on all patients with suspected COVID‐19 presenting at the emergency room of a large academic hospital. Blood drawn within 2 d after hospital presentation was analyzed by point‐of‐care automated flow cytometry and compared with blood samples collected at later time points. COVID‐19 patients did not exhibit neutrophilia or eosinopenia. Unexpectedly neutrophil activation markers (CD11b, CD16, CD10, and CD62L) did not differ between COVID‐19‐positive patients and COVID‐19‐negative patients diagnosed with other bacterial/viral infections, or between COVID‐19 severity groups. In all patients, a decrease was found in the neutrophil maturation markers indicating an inflammation‐induced left shift of the neutrophil compartment. In COVID‐19 this was associated with disease severity.
Graphical
COVID‐19 is associated with a decrease in maturation markers in the neutrophil compartment without clear signs of activation at hospital presentation (figure made with the use of Biorender software). |
| doi_str_mv | 10.1002/JLB.5COVA0820-520RRR |
| format | Article |
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Graphical
COVID‐19 is associated with a decrease in maturation markers in the neutrophil compartment without clear signs of activation at hospital presentation (figure made with the use of Biorender software).</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1002/JLB.5COVA0820-520RRR</identifier><identifier>PMID: 33617030</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>activation ; Aged ; Antigens, CD - blood ; Antigens, CD - immunology ; CD10 ; COVID-19 - blood ; COVID-19 - immunology ; COVID-19 - pathology ; Female ; Flow Cytometry ; Hospitals ; Humans ; Inflammation - blood ; Inflammation - immunology ; Inflammation - pathology ; Male ; Middle Aged ; neprilysin ; neutrophil ; Neutrophil Activation ; Neutrophils - immunology ; Neutrophils - metabolism ; Neutrophils - pathology ; SARS-CoV-2 - immunology ; SARS-CoV-2 - metabolism ; SARS‐CoV‐2</subject><ispartof>Journal of leukocyte biology, 2021-01, Vol.109 (1), p.99-114</ispartof><rights>2020 Society for Leukocyte Biology</rights><rights>2020 Society for Leukocyte Biology.</rights><rights>2020 The Authors. published by Wiley Periodicals LLC on behalf of Society for Leukocyte Biology 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5110-c2b519f00ed964c10f471b0ddecd6cd918bce1ba24f4433987d67cdda8f3ecd13</citedby><cites>FETCH-LOGICAL-c5110-c2b519f00ed964c10f471b0ddecd6cd918bce1ba24f4433987d67cdda8f3ecd13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2FJLB.5COVA0820-520RRR$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2FJLB.5COVA0820-520RRR$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33617030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spijkerman, Roy</creatorcontrib><creatorcontrib>Bongers, Suzanne H.</creatorcontrib><creatorcontrib>Bindels, Bas J. J.</creatorcontrib><creatorcontrib>Tinnevelt, Gerjen H.</creatorcontrib><creatorcontrib>Giustarini, Giulio</creatorcontrib><creatorcontrib>Jorritsma, Nikita K. N.</creatorcontrib><creatorcontrib>Buitenwerf, Wiebe</creatorcontrib><creatorcontrib>Spengler, Daan E. J.</creatorcontrib><creatorcontrib>Delemarre, Eveline M.</creatorcontrib><creatorcontrib>Nierkens, Stefan</creatorcontrib><creatorcontrib>Goor, Harriët M. R.</creatorcontrib><creatorcontrib>Jansen, Jeroen J.</creatorcontrib><creatorcontrib>Vrisekoop, Nienke</creatorcontrib><creatorcontrib>Hietbrink, Falco</creatorcontrib><creatorcontrib>Leenen, Luke P. H.</creatorcontrib><creatorcontrib>Kaasjager, Karin A. H.</creatorcontrib><creatorcontrib>Koenderman, Leo</creatorcontrib><creatorcontrib>COVPACH study group</creatorcontrib><creatorcontrib>the COVPACH study group</creatorcontrib><title>Flow cytometric evaluation of the neutrophil compartment in COVID‐19 at hospital presentation: A normal response to an abnormal situation</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Coronavirus disease 2019 (COVID‐19) is a rapidly emerging pandemic disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Critical COVID‐19 is thought to be associated with a hyper‐inflammatory process that can develop into acute respiratory distress syndrome, a critical disease normally mediated by dysfunctional neutrophils. This study tested the hypothesis whether the neutrophil compartment displays characteristics of hyperinflammation in COVID‐19 patients. Therefore, a prospective study was performed on all patients with suspected COVID‐19 presenting at the emergency room of a large academic hospital. Blood drawn within 2 d after hospital presentation was analyzed by point‐of‐care automated flow cytometry and compared with blood samples collected at later time points. COVID‐19 patients did not exhibit neutrophilia or eosinopenia. Unexpectedly neutrophil activation markers (CD11b, CD16, CD10, and CD62L) did not differ between COVID‐19‐positive patients and COVID‐19‐negative patients diagnosed with other bacterial/viral infections, or between COVID‐19 severity groups. In all patients, a decrease was found in the neutrophil maturation markers indicating an inflammation‐induced left shift of the neutrophil compartment. In COVID‐19 this was associated with disease severity.
Graphical
COVID‐19 is associated with a decrease in maturation markers in the neutrophil compartment without clear signs of activation at hospital presentation (figure made with the use of Biorender software).</description><subject>activation</subject><subject>Aged</subject><subject>Antigens, CD - blood</subject><subject>Antigens, CD - immunology</subject><subject>CD10</subject><subject>COVID-19 - blood</subject><subject>COVID-19 - immunology</subject><subject>COVID-19 - pathology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Inflammation - blood</subject><subject>Inflammation - immunology</subject><subject>Inflammation - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>neprilysin</subject><subject>neutrophil</subject><subject>Neutrophil Activation</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>Neutrophils - pathology</subject><subject>SARS-CoV-2 - immunology</subject><subject>SARS-CoV-2 - metabolism</subject><subject>SARS‐CoV‐2</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAURS1ERaeFP0DISzYpz3biJN2gYaClaKRKI2BrOY7DGDlxsJ1Ws2PfDd_Il9RthhHsWFm6Pu_ep3cRekngjADQN5_W786K1fXXJVQUsoLCZrN5ghakZlXGeMmeogWUOcmKHOAYnYTwHQAY5fAMHTPGSQkMFujuwrpbrHbR9Tp6o7C-kXaS0bgBuw7HrcaDnqJ349ZYrFw_Sh97PURsBpzSr97__vmL1FhGvHVhNFFaPHodEvFoco6XeHC-T3JSRzcEjaPDcsCy2evBxDnwOTrqpA36xf49RV8uPnxefczW15dXq-U6UwUhkCnaFKTuAHRb81wR6PKSNNC2WrVctTWpGqVJI2ne5TljdVW2vFRtK6uOJYSwU_R29h2nptetSrt6acXoTS_9TjhpxL8_g9mKb-5GpLsTXvEiObzeO3j3Y9Ihit4Epa2Vg3ZTEDSvKa2grHhC8xlV3oXgdXfIIfBgSEUqUhyKFHORaezV3zsehv40l4DzGbg1Vu_-y_RBJFBxYPdj6LEc</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Spijkerman, Roy</creator><creator>Bongers, Suzanne H.</creator><creator>Bindels, Bas J. 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H.</creator><creator>Koenderman, Leo</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>202101</creationdate><title>Flow cytometric evaluation of the neutrophil compartment in COVID‐19 at hospital presentation: A normal response to an abnormal situation</title><author>Spijkerman, Roy ; Bongers, Suzanne H. ; Bindels, Bas J. J. ; Tinnevelt, Gerjen H. ; Giustarini, Giulio ; Jorritsma, Nikita K. N. ; Buitenwerf, Wiebe ; Spengler, Daan E. J. ; Delemarre, Eveline M. ; Nierkens, Stefan ; Goor, Harriët M. R. ; Jansen, Jeroen J. ; Vrisekoop, Nienke ; Hietbrink, Falco ; Leenen, Luke P. H. ; Kaasjager, Karin A. 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H.</creatorcontrib><creatorcontrib>Koenderman, Leo</creatorcontrib><creatorcontrib>COVPACH study group</creatorcontrib><creatorcontrib>the COVPACH study group</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spijkerman, Roy</au><au>Bongers, Suzanne H.</au><au>Bindels, Bas J. J.</au><au>Tinnevelt, Gerjen H.</au><au>Giustarini, Giulio</au><au>Jorritsma, Nikita K. N.</au><au>Buitenwerf, Wiebe</au><au>Spengler, Daan E. J.</au><au>Delemarre, Eveline M.</au><au>Nierkens, Stefan</au><au>Goor, Harriët M. R.</au><au>Jansen, Jeroen J.</au><au>Vrisekoop, Nienke</au><au>Hietbrink, Falco</au><au>Leenen, Luke P. H.</au><au>Kaasjager, Karin A. H.</au><au>Koenderman, Leo</au><aucorp>COVPACH study group</aucorp><aucorp>the COVPACH study group</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow cytometric evaluation of the neutrophil compartment in COVID‐19 at hospital presentation: A normal response to an abnormal situation</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2021-01</date><risdate>2021</risdate><volume>109</volume><issue>1</issue><spage>99</spage><epage>114</epage><pages>99-114</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>Coronavirus disease 2019 (COVID‐19) is a rapidly emerging pandemic disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Critical COVID‐19 is thought to be associated with a hyper‐inflammatory process that can develop into acute respiratory distress syndrome, a critical disease normally mediated by dysfunctional neutrophils. This study tested the hypothesis whether the neutrophil compartment displays characteristics of hyperinflammation in COVID‐19 patients. Therefore, a prospective study was performed on all patients with suspected COVID‐19 presenting at the emergency room of a large academic hospital. Blood drawn within 2 d after hospital presentation was analyzed by point‐of‐care automated flow cytometry and compared with blood samples collected at later time points. COVID‐19 patients did not exhibit neutrophilia or eosinopenia. Unexpectedly neutrophil activation markers (CD11b, CD16, CD10, and CD62L) did not differ between COVID‐19‐positive patients and COVID‐19‐negative patients diagnosed with other bacterial/viral infections, or between COVID‐19 severity groups. In all patients, a decrease was found in the neutrophil maturation markers indicating an inflammation‐induced left shift of the neutrophil compartment. In COVID‐19 this was associated with disease severity.
Graphical
COVID‐19 is associated with a decrease in maturation markers in the neutrophil compartment without clear signs of activation at hospital presentation (figure made with the use of Biorender software).</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33617030</pmid><doi>10.1002/JLB.5COVA0820-520RRR</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | activation Aged Antigens, CD - blood Antigens, CD - immunology CD10 COVID-19 - blood COVID-19 - immunology COVID-19 - pathology Female Flow Cytometry Hospitals Humans Inflammation - blood Inflammation - immunology Inflammation - pathology Male Middle Aged neprilysin neutrophil Neutrophil Activation Neutrophils - immunology Neutrophils - metabolism Neutrophils - pathology SARS-CoV-2 - immunology SARS-CoV-2 - metabolism SARS‐CoV‐2 |
| title | Flow cytometric evaluation of the neutrophil compartment in COVID‐19 at hospital presentation: A normal response to an abnormal situation |
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