Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2
Regulatory T cell can protect against severe forms of coronaviral infections attributable to host inflammatory responses. But its role in the pathogenesis of COVID‐19 is still unclear. In this study, frequencies of total and multiple subsets of lymphocytes in peripheral blood of COVID‐19 patients an...
Gespeichert in:
| Veröffentlicht in: | Journal of leukocyte biology 2021-01, Vol.109 (1), p.91-97 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 97 |
|---|---|
| container_issue | 1 |
| container_start_page | 91 |
| container_title | Journal of leukocyte biology |
| container_volume | 109 |
| creator | Gao, Menglu Liu, Yili Guo, Mingquan Wang, Qianying Wang, Yan Fan, Jian Shen, Yinzhong Hou, Junjie Wan, Yanmin Zhu, Zhaoqin |
| description | Regulatory T cell can protect against severe forms of coronaviral infections attributable to host inflammatory responses. But its role in the pathogenesis of COVID‐19 is still unclear. In this study, frequencies of total and multiple subsets of lymphocytes in peripheral blood of COVID‐19 patients and discharged individuals were analyzed using a multicolor flow cytometry assay. Plasma concentration of IL‐10 was measured using a microsphere‐based immunoassay kit. Comparing to healthy controls, the frequencies of total lymphocytes and T cells decreased significantly in both acutely infected COVID‐19 patients and discharged individuals. The frequencies of total lymphocytes correlated negatively with the frequencies of CD3−CD56+ NK cells. The frequencies of regulatory CD8+CD25+ T cells correlated with CD4+/CD8+ T cell ratios positively, while the frequencies of regulatory CD4+CD25+CD127− T cells correlated negatively with CD4+/CD8+ T cell ratios. Ratios of CD4+/CD8+ T cells increased significantly in patients beyond age of 45 years. And accordingly, the frequencies of regulatory CD8+CD25+ T cells were also found significantly increased in these patients. Collectively, the results suggest that regulatory CD4+ and CD8+ T cells may play distinct roles in the pathogenesis of COVID‐19. Moreover, the data indicate that NK cells might contribute to the COVID‐19 associated lymphopenia.
Graphical
Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2 |
| doi_str_mv | 10.1002/JLB.5COVA0720-421RR |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10016821</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2442848390</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5346-b3c8c3c54c83bb9163a32626592164997f89575cb17806cf0a1e8027db03f4ac3</originalsourceid><addsrcrecordid>eNqNkc1OGzEUha2KqqS0T1Cp8hIJDVz_zIy9Qum0_FSRkAJla3kcTzCajFN7BpRdlyz7jH0SHAIR7LqxLft8x_feg9AXAocEgB79nHw7zKuL6zGUFDJOyXT6Do2IZCJjRcl20AhKTrKcA-yijzHeAgCjBXxAu4xKBjwXI_QwtfOh1b0PK1x95wdYd7N0EAf4ChvbthHrYHFn57p3d7ZdYeNDsAmwM3zv-psn6OgVgENS-ohdh5fpZLs-WZihX7Oua6zZkpfj6eW_P38rf51W-gm9b3Qb7efnfQ_9OvlxVZ1lk4vT82o8yUzOeJHVzAjDTM6NYHUtScF06okWuaSk4FKWjZB5mZualAIK04AmVgAtZzWwhmvD9tDxxnc51As7M6nAoFu1DG6hw0p57dTbl87dqLm_U2nmpBCUJIf9Z4fgfw829mrh4rp13Vk_REU5p4ILJiFJ2UZqgo8x2Gb7D4G1IVUpRLUNUT2FmKivr0vcMi-pJYHcCO5da1f_47m-I5Amwh4B9umqDA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2442848390</pqid></control><display><type>article</type><title>Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Gao, Menglu ; Liu, Yili ; Guo, Mingquan ; Wang, Qianying ; Wang, Yan ; Fan, Jian ; Shen, Yinzhong ; Hou, Junjie ; Wan, Yanmin ; Zhu, Zhaoqin</creator><creatorcontrib>Gao, Menglu ; Liu, Yili ; Guo, Mingquan ; Wang, Qianying ; Wang, Yan ; Fan, Jian ; Shen, Yinzhong ; Hou, Junjie ; Wan, Yanmin ; Zhu, Zhaoqin</creatorcontrib><description>Regulatory T cell can protect against severe forms of coronaviral infections attributable to host inflammatory responses. But its role in the pathogenesis of COVID‐19 is still unclear. In this study, frequencies of total and multiple subsets of lymphocytes in peripheral blood of COVID‐19 patients and discharged individuals were analyzed using a multicolor flow cytometry assay. Plasma concentration of IL‐10 was measured using a microsphere‐based immunoassay kit. Comparing to healthy controls, the frequencies of total lymphocytes and T cells decreased significantly in both acutely infected COVID‐19 patients and discharged individuals. The frequencies of total lymphocytes correlated negatively with the frequencies of CD3−CD56+ NK cells. The frequencies of regulatory CD8+CD25+ T cells correlated with CD4+/CD8+ T cell ratios positively, while the frequencies of regulatory CD4+CD25+CD127− T cells correlated negatively with CD4+/CD8+ T cell ratios. Ratios of CD4+/CD8+ T cells increased significantly in patients beyond age of 45 years. And accordingly, the frequencies of regulatory CD8+CD25+ T cells were also found significantly increased in these patients. Collectively, the results suggest that regulatory CD4+ and CD8+ T cells may play distinct roles in the pathogenesis of COVID‐19. Moreover, the data indicate that NK cells might contribute to the COVID‐19 associated lymphopenia.
Graphical
Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1002/JLB.5COVA0720-421RR</identifier><identifier>PMID: 32930458</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adult ; Aged ; Antigens, CD - blood ; Antigens, CD - immunology ; CD4+ T cell ; CD4-CD8 Ratio ; CD8+ T cell ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - metabolism ; CD8-Positive T-Lymphocytes - pathology ; COVID-19 - blood ; COVID-19 - immunology ; COVID-19 - pathology ; COVID‐19 ; Female ; Humans ; Killer Cells, Natural - immunology ; Killer Cells, Natural - pathology ; lymphopenia ; Male ; Middle Aged ; regulatory T cell ; SARS-CoV-2 - immunology ; SARS-CoV-2 - metabolism ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; T-Lymphocytes, Regulatory - pathology</subject><ispartof>Journal of leukocyte biology, 2021-01, Vol.109 (1), p.91-97</ispartof><rights>2020 Society for Leukocyte Biology</rights><rights>2020 Society for Leukocyte Biology.</rights><rights>2020 Society for Leukocyte Biology 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5346-b3c8c3c54c83bb9163a32626592164997f89575cb17806cf0a1e8027db03f4ac3</citedby><cites>FETCH-LOGICAL-c5346-b3c8c3c54c83bb9163a32626592164997f89575cb17806cf0a1e8027db03f4ac3</cites><orcidid>0000-0003-1101-5999 ; 0000-0001-5027-9423</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2FJLB.5COVA0720-421RR$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2FJLB.5COVA0720-421RR$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32930458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Menglu</creatorcontrib><creatorcontrib>Liu, Yili</creatorcontrib><creatorcontrib>Guo, Mingquan</creatorcontrib><creatorcontrib>Wang, Qianying</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Fan, Jian</creatorcontrib><creatorcontrib>Shen, Yinzhong</creatorcontrib><creatorcontrib>Hou, Junjie</creatorcontrib><creatorcontrib>Wan, Yanmin</creatorcontrib><creatorcontrib>Zhu, Zhaoqin</creatorcontrib><title>Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Regulatory T cell can protect against severe forms of coronaviral infections attributable to host inflammatory responses. But its role in the pathogenesis of COVID‐19 is still unclear. In this study, frequencies of total and multiple subsets of lymphocytes in peripheral blood of COVID‐19 patients and discharged individuals were analyzed using a multicolor flow cytometry assay. Plasma concentration of IL‐10 was measured using a microsphere‐based immunoassay kit. Comparing to healthy controls, the frequencies of total lymphocytes and T cells decreased significantly in both acutely infected COVID‐19 patients and discharged individuals. The frequencies of total lymphocytes correlated negatively with the frequencies of CD3−CD56+ NK cells. The frequencies of regulatory CD8+CD25+ T cells correlated with CD4+/CD8+ T cell ratios positively, while the frequencies of regulatory CD4+CD25+CD127− T cells correlated negatively with CD4+/CD8+ T cell ratios. Ratios of CD4+/CD8+ T cells increased significantly in patients beyond age of 45 years. And accordingly, the frequencies of regulatory CD8+CD25+ T cells were also found significantly increased in these patients. Collectively, the results suggest that regulatory CD4+ and CD8+ T cells may play distinct roles in the pathogenesis of COVID‐19. Moreover, the data indicate that NK cells might contribute to the COVID‐19 associated lymphopenia.
Graphical
Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</description><subject>Adult</subject><subject>Aged</subject><subject>Antigens, CD - blood</subject><subject>Antigens, CD - immunology</subject><subject>CD4+ T cell</subject><subject>CD4-CD8 Ratio</subject><subject>CD8+ T cell</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>CD8-Positive T-Lymphocytes - pathology</subject><subject>COVID-19 - blood</subject><subject>COVID-19 - immunology</subject><subject>COVID-19 - pathology</subject><subject>COVID‐19</subject><subject>Female</subject><subject>Humans</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - pathology</subject><subject>lymphopenia</subject><subject>Male</subject><subject>Middle Aged</subject><subject>regulatory T cell</subject><subject>SARS-CoV-2 - immunology</subject><subject>SARS-CoV-2 - metabolism</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>T-Lymphocytes, Regulatory - pathology</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1OGzEUha2KqqS0T1Cp8hIJDVz_zIy9Qum0_FSRkAJla3kcTzCajFN7BpRdlyz7jH0SHAIR7LqxLft8x_feg9AXAocEgB79nHw7zKuL6zGUFDJOyXT6Do2IZCJjRcl20AhKTrKcA-yijzHeAgCjBXxAu4xKBjwXI_QwtfOh1b0PK1x95wdYd7N0EAf4ChvbthHrYHFn57p3d7ZdYeNDsAmwM3zv-psn6OgVgENS-ohdh5fpZLs-WZihX7Oua6zZkpfj6eW_P38rf51W-gm9b3Qb7efnfQ_9OvlxVZ1lk4vT82o8yUzOeJHVzAjDTM6NYHUtScF06okWuaSk4FKWjZB5mZualAIK04AmVgAtZzWwhmvD9tDxxnc51As7M6nAoFu1DG6hw0p57dTbl87dqLm_U2nmpBCUJIf9Z4fgfw829mrh4rp13Vk_REU5p4ILJiFJ2UZqgo8x2Gb7D4G1IVUpRLUNUT2FmKivr0vcMi-pJYHcCO5da1f_47m-I5Amwh4B9umqDA</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Gao, Menglu</creator><creator>Liu, Yili</creator><creator>Guo, Mingquan</creator><creator>Wang, Qianying</creator><creator>Wang, Yan</creator><creator>Fan, Jian</creator><creator>Shen, Yinzhong</creator><creator>Hou, Junjie</creator><creator>Wan, Yanmin</creator><creator>Zhu, Zhaoqin</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1101-5999</orcidid><orcidid>https://orcid.org/0000-0001-5027-9423</orcidid></search><sort><creationdate>202101</creationdate><title>Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</title><author>Gao, Menglu ; Liu, Yili ; Guo, Mingquan ; Wang, Qianying ; Wang, Yan ; Fan, Jian ; Shen, Yinzhong ; Hou, Junjie ; Wan, Yanmin ; Zhu, Zhaoqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5346-b3c8c3c54c83bb9163a32626592164997f89575cb17806cf0a1e8027db03f4ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antigens, CD - blood</topic><topic>Antigens, CD - immunology</topic><topic>CD4+ T cell</topic><topic>CD4-CD8 Ratio</topic><topic>CD8+ T cell</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>CD8-Positive T-Lymphocytes - pathology</topic><topic>COVID-19 - blood</topic><topic>COVID-19 - immunology</topic><topic>COVID-19 - pathology</topic><topic>COVID‐19</topic><topic>Female</topic><topic>Humans</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - pathology</topic><topic>lymphopenia</topic><topic>Male</topic><topic>Middle Aged</topic><topic>regulatory T cell</topic><topic>SARS-CoV-2 - immunology</topic><topic>SARS-CoV-2 - metabolism</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>T-Lymphocytes, Regulatory - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Menglu</creatorcontrib><creatorcontrib>Liu, Yili</creatorcontrib><creatorcontrib>Guo, Mingquan</creatorcontrib><creatorcontrib>Wang, Qianying</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Fan, Jian</creatorcontrib><creatorcontrib>Shen, Yinzhong</creatorcontrib><creatorcontrib>Hou, Junjie</creatorcontrib><creatorcontrib>Wan, Yanmin</creatorcontrib><creatorcontrib>Zhu, Zhaoqin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Menglu</au><au>Liu, Yili</au><au>Guo, Mingquan</au><au>Wang, Qianying</au><au>Wang, Yan</au><au>Fan, Jian</au><au>Shen, Yinzhong</au><au>Hou, Junjie</au><au>Wan, Yanmin</au><au>Zhu, Zhaoqin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2021-01</date><risdate>2021</risdate><volume>109</volume><issue>1</issue><spage>91</spage><epage>97</epage><pages>91-97</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>Regulatory T cell can protect against severe forms of coronaviral infections attributable to host inflammatory responses. But its role in the pathogenesis of COVID‐19 is still unclear. In this study, frequencies of total and multiple subsets of lymphocytes in peripheral blood of COVID‐19 patients and discharged individuals were analyzed using a multicolor flow cytometry assay. Plasma concentration of IL‐10 was measured using a microsphere‐based immunoassay kit. Comparing to healthy controls, the frequencies of total lymphocytes and T cells decreased significantly in both acutely infected COVID‐19 patients and discharged individuals. The frequencies of total lymphocytes correlated negatively with the frequencies of CD3−CD56+ NK cells. The frequencies of regulatory CD8+CD25+ T cells correlated with CD4+/CD8+ T cell ratios positively, while the frequencies of regulatory CD4+CD25+CD127− T cells correlated negatively with CD4+/CD8+ T cell ratios. Ratios of CD4+/CD8+ T cells increased significantly in patients beyond age of 45 years. And accordingly, the frequencies of regulatory CD8+CD25+ T cells were also found significantly increased in these patients. Collectively, the results suggest that regulatory CD4+ and CD8+ T cells may play distinct roles in the pathogenesis of COVID‐19. Moreover, the data indicate that NK cells might contribute to the COVID‐19 associated lymphopenia.
Graphical
Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>32930458</pmid><doi>10.1002/JLB.5COVA0720-421RR</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1101-5999</orcidid><orcidid>https://orcid.org/0000-0001-5027-9423</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0741-5400 |
| ispartof | Journal of leukocyte biology, 2021-01, Vol.109 (1), p.91-97 |
| issn | 0741-5400 1938-3673 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10016821 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Adult Aged Antigens, CD - blood Antigens, CD - immunology CD4+ T cell CD4-CD8 Ratio CD8+ T cell CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - pathology COVID-19 - blood COVID-19 - immunology COVID-19 - pathology COVID‐19 Female Humans Killer Cells, Natural - immunology Killer Cells, Natural - pathology lymphopenia Male Middle Aged regulatory T cell SARS-CoV-2 - immunology SARS-CoV-2 - metabolism T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism T-Lymphocytes, Regulatory - pathology |
| title | Regulatory CD4+ and CD8+ T cells are negatively correlated with CD4+/CD8+ T cell ratios in patients acutely infected with SARS‐CoV‐2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T17%3A42%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulatory%20CD4+%20and%20CD8+%20T%20cells%20are%20negatively%20correlated%20with%20CD4+/CD8+%20T%20cell%20ratios%20in%20patients%20acutely%20infected%20with%20SARS%E2%80%90CoV%E2%80%902&rft.jtitle=Journal%20of%20leukocyte%20biology&rft.au=Gao,%20Menglu&rft.date=2021-01&rft.volume=109&rft.issue=1&rft.spage=91&rft.epage=97&rft.pages=91-97&rft.issn=0741-5400&rft.eissn=1938-3673&rft_id=info:doi/10.1002/JLB.5COVA0720-421RR&rft_dat=%3Cproquest_pubme%3E2442848390%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2442848390&rft_id=info:pmid/32930458&rfr_iscdi=true |