Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
Background No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with...
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| Veröffentlicht in: | Digestive diseases and sciences 2023-03, Vol.68 (3), p.813-823 |
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| creator | Sugai, Tamotsu Sugimoto, Ryo Eizuka, Makoto Osakabe, Mitsumasa Yamada, Shun Yanagawa, Naoki Matsumoto, Takayuki Suzuki, Hiromu |
| description | Background
No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with the onset of cancer invasion and progression.
Aims
We hypothesized that the identification of miRs associated with CRC might be useful to detect this disease at early stages.
Methods
We conducted an integrated analysis of 79 isolated colorectal tumor glands, including adenomas, intramucosal cancers, and invasive CRCs that showed a microsatellite stable phenotype using GeneChip miRNA 4.0 microarray assays. The colorectal tumors we examined were divided into 2 cohorts (42 in the first cohort and 37 in the second cohort).
Results
First, cluster analysis was performed to stratify expression patterns of multiple miRs that were pooled according to the following criteria: fold change in expression ( 2.0),
p
|
| doi_str_mv | 10.1007/s10620-022-07576-8 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10011343</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A741190302</galeid><sourcerecordid>A741190302</sourcerecordid><originalsourceid>FETCH-LOGICAL-c608t-b89bbc24686c5010a1953c73177173b180bef9955eb22d8dd585175136643bc13</originalsourceid><addsrcrecordid>eNp9kk1v1DAQhi0EokvhD3BAkbhwSZmx44-c0GopH1IFCJUDJyvxOruuknixk4r-e2bZflCEkA-2xs-89rwzjD1HOEEA_TojKA4lcF6CllqV5gFboNSi5FKZh2wBqOiMqI7Yk5wvAKDWqB6zIyGVrupaLtj3VRx2yW_9mMOlL5Zj01_lkIvYFUNwKX79tCxOfxKRc4hj8XZOYdwU09YXX1Lc3ISJXsU-Ju-mpi_O5yGm_JQ96po--2fX-zH79u70fPWhPPv8_uNqeVY6BWYqW1O3reOVMspJQGiwlsJpgVqjFi0aaH1HX5W-5Xxt1mtpJGqJQqlKtA7FMXtz0N3N7eDXzo9Tanq7S2Fo0pWNTbD3b8awtZt4aclDRFEJUnh1rZDij9nnyQ4hO9_3zejjnC0ns0QleSUJffkXehHnRKYRpY0SQkGFd9Sm6b0NYxfpYbcXtUtdIdYggBN18g-K1tqT9XH0XaD4vQR-SKC-5Jx8d1skwr4abQ8TYWki7O-JsIaSXvxpz23KzQgQIA5A3u1769NdSf-R_QXf3b9P</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2786336041</pqid></control><display><type>article</type><title>Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Sugai, Tamotsu ; Sugimoto, Ryo ; Eizuka, Makoto ; Osakabe, Mitsumasa ; Yamada, Shun ; Yanagawa, Naoki ; Matsumoto, Takayuki ; Suzuki, Hiromu</creator><creatorcontrib>Sugai, Tamotsu ; Sugimoto, Ryo ; Eizuka, Makoto ; Osakabe, Mitsumasa ; Yamada, Shun ; Yanagawa, Naoki ; Matsumoto, Takayuki ; Suzuki, Hiromu</creatorcontrib><description>Background
No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with the onset of cancer invasion and progression.
Aims
We hypothesized that the identification of miRs associated with CRC might be useful to detect this disease at early stages.
Methods
We conducted an integrated analysis of 79 isolated colorectal tumor glands, including adenomas, intramucosal cancers, and invasive CRCs that showed a microsatellite stable phenotype using GeneChip miRNA 4.0 microarray assays. The colorectal tumors we examined were divided into 2 cohorts (42 in the first cohort and 37 in the second cohort).
Results
First, cluster analysis was performed to stratify expression patterns of multiple miRs that were pooled according to the following criteria: fold change in expression (< −2.0 or > 2.0),
p
< 0.05, and mature miRs. As a result, the expression patterns of pooled miRs were subdivided into 3 subgroups that were correlated with tumor grade. Each subgroup was characterized by specific miRs. In addition, we found that specific miRs, including
miR-140-3p
and
miR-378i
, were closely associated with cancer invasion. Finally, we analyzed paired dysregulated miRs between adenomatous and cancerous components present within the same tumor.
Discussion
We showed that several miRs were dysregulated during progression from adenoma to intramucosal cancer. Specific miRs may have key roles in progression from intramucosal tumor to invasive CRC.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1007/s10620-022-07576-8</identifier><identifier>PMID: 35674995</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenoma - diagnosis ; Analysis ; Biochemistry ; Biomarkers ; Biomarkers, Tumor - genetics ; Cancer ; Colorectal cancer ; Colorectal Neoplasms - diagnosis ; Development and progression ; Gastroenterology ; Gastrointestinal diseases ; Gene Expression Regulation, Neoplastic ; Hepatology ; Humans ; Medicine ; Medicine & Public Health ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; Oligonucleotide Array Sequence Analysis ; Oncology ; Oncology, Experimental ; Original ; Original Article ; Transplant Surgery ; Tumors</subject><ispartof>Digestive diseases and sciences, 2023-03, Vol.68 (3), p.813-823</ispartof><rights>The Author(s) 2022</rights><rights>2022. The Author(s).</rights><rights>COPYRIGHT 2023 Springer</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-b89bbc24686c5010a1953c73177173b180bef9955eb22d8dd585175136643bc13</citedby><cites>FETCH-LOGICAL-c608t-b89bbc24686c5010a1953c73177173b180bef9955eb22d8dd585175136643bc13</cites><orcidid>0000-0002-4896-3557</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10620-022-07576-8$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10620-022-07576-8$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35674995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugai, Tamotsu</creatorcontrib><creatorcontrib>Sugimoto, Ryo</creatorcontrib><creatorcontrib>Eizuka, Makoto</creatorcontrib><creatorcontrib>Osakabe, Mitsumasa</creatorcontrib><creatorcontrib>Yamada, Shun</creatorcontrib><creatorcontrib>Yanagawa, Naoki</creatorcontrib><creatorcontrib>Matsumoto, Takayuki</creatorcontrib><creatorcontrib>Suzuki, Hiromu</creatorcontrib><title>Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><addtitle>Dig Dis Sci</addtitle><description>Background
No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with the onset of cancer invasion and progression.
Aims
We hypothesized that the identification of miRs associated with CRC might be useful to detect this disease at early stages.
Methods
We conducted an integrated analysis of 79 isolated colorectal tumor glands, including adenomas, intramucosal cancers, and invasive CRCs that showed a microsatellite stable phenotype using GeneChip miRNA 4.0 microarray assays. The colorectal tumors we examined were divided into 2 cohorts (42 in the first cohort and 37 in the second cohort).
Results
First, cluster analysis was performed to stratify expression patterns of multiple miRs that were pooled according to the following criteria: fold change in expression (< −2.0 or > 2.0),
p
< 0.05, and mature miRs. As a result, the expression patterns of pooled miRs were subdivided into 3 subgroups that were correlated with tumor grade. Each subgroup was characterized by specific miRs. In addition, we found that specific miRs, including
miR-140-3p
and
miR-378i
, were closely associated with cancer invasion. Finally, we analyzed paired dysregulated miRs between adenomatous and cancerous components present within the same tumor.
Discussion
We showed that several miRs were dysregulated during progression from adenoma to intramucosal cancer. Specific miRs may have key roles in progression from intramucosal tumor to invasive CRC.</description><subject>Adenoma - diagnosis</subject><subject>Analysis</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Cancer</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - diagnosis</subject><subject>Development and progression</subject><subject>Gastroenterology</subject><subject>Gastrointestinal diseases</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hepatology</subject><subject>Humans</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Oncology</subject><subject>Oncology, Experimental</subject><subject>Original</subject><subject>Original Article</subject><subject>Transplant Surgery</subject><subject>Tumors</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kk1v1DAQhi0EokvhD3BAkbhwSZmx44-c0GopH1IFCJUDJyvxOruuknixk4r-e2bZflCEkA-2xs-89rwzjD1HOEEA_TojKA4lcF6CllqV5gFboNSi5FKZh2wBqOiMqI7Yk5wvAKDWqB6zIyGVrupaLtj3VRx2yW_9mMOlL5Zj01_lkIvYFUNwKX79tCxOfxKRc4hj8XZOYdwU09YXX1Lc3ISJXsU-Ju-mpi_O5yGm_JQ96po--2fX-zH79u70fPWhPPv8_uNqeVY6BWYqW1O3reOVMspJQGiwlsJpgVqjFi0aaH1HX5W-5Xxt1mtpJGqJQqlKtA7FMXtz0N3N7eDXzo9Tanq7S2Fo0pWNTbD3b8awtZt4aclDRFEJUnh1rZDij9nnyQ4hO9_3zejjnC0ns0QleSUJffkXehHnRKYRpY0SQkGFd9Sm6b0NYxfpYbcXtUtdIdYggBN18g-K1tqT9XH0XaD4vQR-SKC-5Jx8d1skwr4abQ8TYWki7O-JsIaSXvxpz23KzQgQIA5A3u1769NdSf-R_QXf3b9P</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Sugai, Tamotsu</creator><creator>Sugimoto, Ryo</creator><creator>Eizuka, Makoto</creator><creator>Osakabe, Mitsumasa</creator><creator>Yamada, Shun</creator><creator>Yanagawa, Naoki</creator><creator>Matsumoto, Takayuki</creator><creator>Suzuki, Hiromu</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4896-3557</orcidid></search><sort><creationdate>20230301</creationdate><title>Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors</title><author>Sugai, Tamotsu ; Sugimoto, Ryo ; Eizuka, Makoto ; Osakabe, Mitsumasa ; Yamada, Shun ; Yanagawa, Naoki ; Matsumoto, Takayuki ; Suzuki, Hiromu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c608t-b89bbc24686c5010a1953c73177173b180bef9955eb22d8dd585175136643bc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adenoma - diagnosis</topic><topic>Analysis</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Cancer</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - diagnosis</topic><topic>Development and progression</topic><topic>Gastroenterology</topic><topic>Gastrointestinal diseases</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hepatology</topic><topic>Humans</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Oncology</topic><topic>Oncology, Experimental</topic><topic>Original</topic><topic>Original Article</topic><topic>Transplant Surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugai, Tamotsu</creatorcontrib><creatorcontrib>Sugimoto, Ryo</creatorcontrib><creatorcontrib>Eizuka, Makoto</creatorcontrib><creatorcontrib>Osakabe, Mitsumasa</creatorcontrib><creatorcontrib>Yamada, Shun</creatorcontrib><creatorcontrib>Yanagawa, Naoki</creatorcontrib><creatorcontrib>Matsumoto, Takayuki</creatorcontrib><creatorcontrib>Suzuki, Hiromu</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugai, Tamotsu</au><au>Sugimoto, Ryo</au><au>Eizuka, Makoto</au><au>Osakabe, Mitsumasa</au><au>Yamada, Shun</au><au>Yanagawa, Naoki</au><au>Matsumoto, Takayuki</au><au>Suzuki, Hiromu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors</atitle><jtitle>Digestive diseases and sciences</jtitle><stitle>Dig Dis Sci</stitle><addtitle>Dig Dis Sci</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>68</volume><issue>3</issue><spage>813</spage><epage>823</epage><pages>813-823</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><abstract>Background
No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with the onset of cancer invasion and progression.
Aims
We hypothesized that the identification of miRs associated with CRC might be useful to detect this disease at early stages.
Methods
We conducted an integrated analysis of 79 isolated colorectal tumor glands, including adenomas, intramucosal cancers, and invasive CRCs that showed a microsatellite stable phenotype using GeneChip miRNA 4.0 microarray assays. The colorectal tumors we examined were divided into 2 cohorts (42 in the first cohort and 37 in the second cohort).
Results
First, cluster analysis was performed to stratify expression patterns of multiple miRs that were pooled according to the following criteria: fold change in expression (< −2.0 or > 2.0),
p
< 0.05, and mature miRs. As a result, the expression patterns of pooled miRs were subdivided into 3 subgroups that were correlated with tumor grade. Each subgroup was characterized by specific miRs. In addition, we found that specific miRs, including
miR-140-3p
and
miR-378i
, were closely associated with cancer invasion. Finally, we analyzed paired dysregulated miRs between adenomatous and cancerous components present within the same tumor.
Discussion
We showed that several miRs were dysregulated during progression from adenoma to intramucosal cancer. Specific miRs may have key roles in progression from intramucosal tumor to invasive CRC.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35674995</pmid><doi>10.1007/s10620-022-07576-8</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4896-3557</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Digestive diseases and sciences, 2023-03, Vol.68 (3), p.813-823 |
| issn | 0163-2116 1573-2568 |
| language | eng |
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| subjects | Adenoma - diagnosis Analysis Biochemistry Biomarkers Biomarkers, Tumor - genetics Cancer Colorectal cancer Colorectal Neoplasms - diagnosis Development and progression Gastroenterology Gastrointestinal diseases Gene Expression Regulation, Neoplastic Hepatology Humans Medicine Medicine & Public Health MicroRNA MicroRNAs MicroRNAs - genetics Oligonucleotide Array Sequence Analysis Oncology Oncology, Experimental Original Original Article Transplant Surgery Tumors |
| title | Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors |
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