Orientation-dependent and sequence-specific expansions of CTG/CAG trinucleotide repeats in Saccharomyces cerevisiae

A quantitative and selective genetic assay was developed to monitor expansions of trinucleotide repeats (TNRs) in yeast. A promoter containing 25 repeats allows expression of a URA3 reporter gene and yields sensitivity to the drug 5-fluoroorotic acid. Expansion of the TNR to 30 or more repeats turns...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-10, Vol.95 (21), p.12438-12443
Hauptverfasser:	Miret, J.J. (University of Nebraska Medical Center, Omaha, NE.), Pessoa-Brandao, L, Lahue, R.S
Format:	Artikel
Sprache:	eng
Schlagworte:	5-FLUOROORTIC ACID ABERRANT DNA REPLICATION ADN Alleles Base Sequence Biological Sciences DNA DNA CONFORMATION DNA HAIRPINS DNA Primers DNA REPLICATION DRUGS EXPRESION GENICA EXPRESSION DES GENES GENE GENE EXPRESSION GENES Genetic loci Genetics LARGURA LENGTH LONGUEUR MEDICAMENT MEDICAMENTOS Microsatellite repeats NUCLEOTIDE SEQUENCE ORIENTACION ORIENTATION Plasmids Polymerase Chain Reaction PROMOTERS Quantitative genetics REPETITIVE DNA REPETITIVE DNA EXPANSIONS REPLICACION REPLICATION REPORTER GENES RESISTANCE AUX PRODUITS CHIMIQUES RESISTANCE TO CHEMICALS RESISTENCIA A PRODUCTOS QUIMICOS SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE Trinucleotide Repeats URA3 GENE Yeast Yeasts
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