Site-Specific Gene Delivery in vivo through Engineered Sendai Viral Envelopes
Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery o...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-09, Vol.95 (20), p.11886-11890 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Ramani, Komal Hassan, Quamarul Venkaiah, Betapudi Hasnain, Seyed E. Sarkar, Debi P. |
| description | Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cells of BALB/c mouse in vivo. The membrane fusion-mediated high efficiency of gene transfer to liver cells was ascertained following a critical evaluation of the level of the DNA, mRNA, and relevant proteins. Furthermore, the involvement of viral glycoprotein both as a unique natural ligand and as a membrane fusogen could lead to preferential transfection of parenchymal cell types of liver. The integration of transgenes in the mouse chromosomal DNA and its stable expression up to 4 mo after single i.v. administration of this gene carrier has bolstered its efficiency and novelty. Moreover, the F-virosomes did not elicit significant humoral immune response against the fusion protein in the injected animal. The findings reported here open up the possibility for considering "F-virosomes" as a promising "vehicle" for sitespecific DNA delivery in gene therapy. |
| doi_str_mv | 10.1073/pnas.95.20.11886 |
| format | Article |
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We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cells of BALB/c mouse in vivo. The membrane fusion-mediated high efficiency of gene transfer to liver cells was ascertained following a critical evaluation of the level of the DNA, mRNA, and relevant proteins. Furthermore, the involvement of viral glycoprotein both as a unique natural ligand and as a membrane fusogen could lead to preferential transfection of parenchymal cell types of liver. The integration of transgenes in the mouse chromosomal DNA and its stable expression up to 4 mo after single i.v. administration of this gene carrier has bolstered its efficiency and novelty. Moreover, the F-virosomes did not elicit significant humoral immune response against the fusion protein in the injected animal. The findings reported here open up the possibility for considering "F-virosomes" as a promising "vehicle" for sitespecific DNA delivery in gene therapy.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.95.20.11886</identifier><identifier>PMID: 9751760</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Biological Sciences ; Cells ; Chloramphenicol O-Acetyltransferase - genetics ; Chromosomes - genetics ; DNA ; DNA - administration & dosage ; DNA - genetics ; Female ; Gene Expression ; Genes ; Genes, Reporter ; Genetic Engineering ; Genetic Vectors ; Genetics ; Hepatocytes ; Liver ; Liver - cytology ; Liver - enzymology ; Liver cells ; Luciferases - genetics ; Membrane Fusion ; Mice ; Mice, Inbred BALB C ; Plasmids ; Polymerase chain reaction ; Respirovirus - genetics ; Reverse transcriptase polymerase chain reaction ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1998-09, Vol.95 (20), p.11886-11890</ispartof><rights>Copyright 1993-1998 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 29, 1998</rights><rights>Copyright © 1998, The National Academy of Sciences 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-65eff2dce2bb6a86b83548e27eec83990e5576ca379a3e9318a040722100841a3</citedby><cites>FETCH-LOGICAL-c523t-65eff2dce2bb6a86b83548e27eec83990e5576ca379a3e9318a040722100841a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/95/20.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/49282$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/49282$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27915,27916,53782,53784,58008,58241</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9751760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramani, Komal</creatorcontrib><creatorcontrib>Hassan, Quamarul</creatorcontrib><creatorcontrib>Venkaiah, Betapudi</creatorcontrib><creatorcontrib>Hasnain, Seyed E.</creatorcontrib><creatorcontrib>Sarkar, Debi P.</creatorcontrib><title>Site-Specific Gene Delivery in vivo through Engineered Sendai Viral Envelopes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cells of BALB/c mouse in vivo. The membrane fusion-mediated high efficiency of gene transfer to liver cells was ascertained following a critical evaluation of the level of the DNA, mRNA, and relevant proteins. Furthermore, the involvement of viral glycoprotein both as a unique natural ligand and as a membrane fusogen could lead to preferential transfection of parenchymal cell types of liver. The integration of transgenes in the mouse chromosomal DNA and its stable expression up to 4 mo after single i.v. administration of this gene carrier has bolstered its efficiency and novelty. Moreover, the F-virosomes did not elicit significant humoral immune response against the fusion protein in the injected animal. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1998-09, Vol.95 (20), p.11886-11890 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological Sciences Cells Chloramphenicol O-Acetyltransferase - genetics Chromosomes - genetics DNA DNA - administration & dosage DNA - genetics Female Gene Expression Genes Genes, Reporter Genetic Engineering Genetic Vectors Genetics Hepatocytes Liver Liver - cytology Liver - enzymology Liver cells Luciferases - genetics Membrane Fusion Mice Mice, Inbred BALB C Plasmids Polymerase chain reaction Respirovirus - genetics Reverse transcriptase polymerase chain reaction Viruses |
| title | Site-Specific Gene Delivery in vivo through Engineered Sendai Viral Envelopes |
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