Histologic Demonstration of Glycosaminoglycans in Inner Ear Fluids
Hyaluronic acid is a glycosaminoglycan (GAG) which has been demonstrated by biochemical and histochemical methods to be present in inner ear fluids and tissues. However, consistent labeling of hyaluronic acid or other GAGs in inner ear fluid compartments in histologic sections has not been previousl...
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| Veröffentlicht in: | Acta oto-laryngologica 1998-07, Vol.118 (4), p.505-510 |
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| creator | PAWLOWSKI, K. S WRIGHT, C. G MEYERHOFF, W. L |
| description | Hyaluronic acid is a glycosaminoglycan (GAG) which has been demonstrated by biochemical and histochemical methods to be present in inner ear fluids and tissues. However, consistent labeling of hyaluronic acid or other GAGs in inner ear fluid compartments in histologic sections has not been previously reported. Staining and characterization of GAGs in the normal inner ear fluids of humans and guinea pigs are described in this report. It was initially observed that alcian blue produces dense staining of the endolymphatic and perilymphatic fluid compartments in celloidin embedded material. Results obtained with alcian blue were subsequently compared to staining obtained with hematoxylin/eosin and periodic acid/Schiff base. Enzyme digestion was also performed with testicular hyaluronidase (bovine and ovine), streptomyces hyaluronidase, chondroitase ABC, and alpha-amylase. Results of the differential staining and enzyme digestion studies suggest that the substances in inner ear fluids that stain with alcian blue are a combination of chondroitin sulfate-4, -5 and/or -6 and hyaluronic acid. |
| doi_str_mv | 10.1080/00016489850154630 |
| format | Article |
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S ; WRIGHT, C. G ; MEYERHOFF, W. L</creator><creatorcontrib>PAWLOWSKI, K. S ; WRIGHT, C. G ; MEYERHOFF, W. L</creatorcontrib><description>Hyaluronic acid is a glycosaminoglycan (GAG) which has been demonstrated by biochemical and histochemical methods to be present in inner ear fluids and tissues. However, consistent labeling of hyaluronic acid or other GAGs in inner ear fluid compartments in histologic sections has not been previously reported. Staining and characterization of GAGs in the normal inner ear fluids of humans and guinea pigs are described in this report. It was initially observed that alcian blue produces dense staining of the endolymphatic and perilymphatic fluid compartments in celloidin embedded material. Results obtained with alcian blue were subsequently compared to staining obtained with hematoxylin/eosin and periodic acid/Schiff base. Enzyme digestion was also performed with testicular hyaluronidase (bovine and ovine), streptomyces hyaluronidase, chondroitase ABC, and alpha-amylase. Results of the differential staining and enzyme digestion studies suggest that the substances in inner ear fluids that stain with alcian blue are a combination of chondroitin sulfate-4, -5 and/or -6 and hyaluronic acid.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.1080/00016489850154630</identifier><identifier>PMID: 9726674</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>Alcian Blue ; Animals ; Biological and medical sciences ; Cattle ; Collodion ; Coloring Agents ; Ent. Stomatology ; Glycosaminoglycans - analysis ; Guinea Pigs ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Labyrinthine Fluids - chemistry ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Sheep ; Staining and Labeling ; Streptomyces ; Tissue Embedding</subject><ispartof>Acta oto-laryngologica, 1998-07, Vol.118 (4), p.505-510</ispartof><rights>1998 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-9d5f4806fd821fda271778d09d2a8f6f7b940d632383a27ddddce8557065c05c3</citedby><cites>FETCH-LOGICAL-c461t-9d5f4806fd821fda271778d09d2a8f6f7b940d632383a27ddddce8557065c05c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00016489850154630$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00016489850154630$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2315740$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9726674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PAWLOWSKI, K. S</creatorcontrib><creatorcontrib>WRIGHT, C. G</creatorcontrib><creatorcontrib>MEYERHOFF, W. L</creatorcontrib><title>Histologic Demonstration of Glycosaminoglycans in Inner Ear Fluids</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Hyaluronic acid is a glycosaminoglycan (GAG) which has been demonstrated by biochemical and histochemical methods to be present in inner ear fluids and tissues. However, consistent labeling of hyaluronic acid or other GAGs in inner ear fluid compartments in histologic sections has not been previously reported. Staining and characterization of GAGs in the normal inner ear fluids of humans and guinea pigs are described in this report. It was initially observed that alcian blue produces dense staining of the endolymphatic and perilymphatic fluid compartments in celloidin embedded material. Results obtained with alcian blue were subsequently compared to staining obtained with hematoxylin/eosin and periodic acid/Schiff base. Enzyme digestion was also performed with testicular hyaluronidase (bovine and ovine), streptomyces hyaluronidase, chondroitase ABC, and alpha-amylase. Results of the differential staining and enzyme digestion studies suggest that the substances in inner ear fluids that stain with alcian blue are a combination of chondroitin sulfate-4, -5 and/or -6 and hyaluronic acid.</description><subject>Alcian Blue</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Collodion</subject><subject>Coloring Agents</subject><subject>Ent. Stomatology</subject><subject>Glycosaminoglycans - analysis</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Labyrinthine Fluids - chemistry</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Sheep</subject><subject>Staining and Labeling</subject><subject>Streptomyces</subject><subject>Tissue Embedding</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlLxDAcxYMoOi4fwIPQg3irZmmWoheXcRQEL3ouMcsYSRNNWmS-vS0zDoiguSTh_d7jnxcADhE8RVDAMwghYpWoBYWIVozADTBBjKISY4o2wWTUyxHYAbs5v43Xgd0G2zXHjPFqAq7uXO6ij3OnihvTxpC7JDsXQxFtMfMLFbNsXYjz4ShDLlwo7kMwqZjKVNz63um8D7as9NkcrPY98Hw7fbq-Kx8eZ_fXlw-lqhjqylpTWwnIrBYYWS0xR5wLDWuNpbDM8pe6gpoRTAQZRD0sZQSlHDKqIFVkD5wsc99T_OhN7prWZWW8l8HEPjecCFEhSv4FMawpqjEeQLQEVYo5J2Ob9-RamRYNgs1YcPOr4MFztArvX1qj145Vo4N-vNJlVtLbJINyeY1hgiivxpiLJeaCjamVnzF53XRy4WP69pC_pjj_YX810nevSibTvMU-heEf_njDFyJDpqE</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>PAWLOWSKI, K. S</creator><creator>WRIGHT, C. G</creator><creator>MEYERHOFF, W. L</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>C1K</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>19980701</creationdate><title>Histologic Demonstration of Glycosaminoglycans in Inner Ear Fluids</title><author>PAWLOWSKI, K. S ; WRIGHT, C. G ; MEYERHOFF, W. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-9d5f4806fd821fda271778d09d2a8f6f7b940d632383a27ddddce8557065c05c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alcian Blue</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Collodion</topic><topic>Coloring Agents</topic><topic>Ent. Stomatology</topic><topic>Glycosaminoglycans - analysis</topic><topic>Guinea Pigs</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Labyrinthine Fluids - chemistry</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Sheep</topic><topic>Staining and Labeling</topic><topic>Streptomyces</topic><topic>Tissue Embedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PAWLOWSKI, K. S</creatorcontrib><creatorcontrib>WRIGHT, C. G</creatorcontrib><creatorcontrib>MEYERHOFF, W. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histologic Demonstration of Glycosaminoglycans in Inner Ear Fluids</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>118</volume><issue>4</issue><spage>505</spage><epage>510</epage><pages>505-510</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>Hyaluronic acid is a glycosaminoglycan (GAG) which has been demonstrated by biochemical and histochemical methods to be present in inner ear fluids and tissues. However, consistent labeling of hyaluronic acid or other GAGs in inner ear fluid compartments in histologic sections has not been previously reported. Staining and characterization of GAGs in the normal inner ear fluids of humans and guinea pigs are described in this report. It was initially observed that alcian blue produces dense staining of the endolymphatic and perilymphatic fluid compartments in celloidin embedded material. Results obtained with alcian blue were subsequently compared to staining obtained with hematoxylin/eosin and periodic acid/Schiff base. Enzyme digestion was also performed with testicular hyaluronidase (bovine and ovine), streptomyces hyaluronidase, chondroitase ABC, and alpha-amylase. Results of the differential staining and enzyme digestion studies suggest that the substances in inner ear fluids that stain with alcian blue are a combination of chondroitin sulfate-4, -5 and/or -6 and hyaluronic acid.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>9726674</pmid><doi>10.1080/00016489850154630</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Alcian Blue Animals Biological and medical sciences Cattle Collodion Coloring Agents Ent. Stomatology Glycosaminoglycans - analysis Guinea Pigs Humans Investigative techniques, diagnostic techniques (general aspects) Labyrinthine Fluids - chemistry Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Sheep Staining and Labeling Streptomyces Tissue Embedding |
| title | Histologic Demonstration of Glycosaminoglycans in Inner Ear Fluids |
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