Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from the strawberry (Fragaria x ananassa)
We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region,...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-07, Vol.62 (7), p.1358-1363 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon) Chung, W.I |
| description | We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. The ApxSC genomic DNA contains 10 exons and 9 introns, which is similar to the structure of pea ApxI. A primer extension analysis suggested that the transcription of ApxSC gene was started at three s tart sites with different degrees. The promoter region of ApxSC gene contains a sequence or structure distinct from other reported plant ascorbate peroxidase genes, though with several known functional elements such as a TATA box |
| doi_str_mv | 10.1271/bbb.62.1358 |
| format | Article |
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(Korea Advanced Inst. of Science and Technology, Taejon) ; Chung, W.I</creator><creatorcontrib>Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon) ; Chung, W.I</creatorcontrib><description>We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. The ApxSC genomic DNA contains 10 exons and 9 introns, which is similar to the structure of pea ApxI. A primer extension analysis suggested that the transcription of ApxSC gene was started at three s tart sites with different degrees. The promoter region of ApxSC gene contains a sequence or structure distinct from other reported plant ascorbate peroxidase genes, though with several known functional elements such as a TATA box</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.62.1358</identifier><identifier>PMID: 9720218</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ACIDE ASCORBIQUE ; ACIDO ASCORBICO ; ADN ; Amino Acid Sequence ; Arabidopsis - enzymology ; Ascorbate Peroxidases ; ASCORBIC ACID ; Base Sequence ; Biological and medical sciences ; Cytosol - enzymology ; cytosolic ascorbate peroxidase ; DNA ; DNA, Plant - chemistry ; DNA, Plant - isolation & purification ; FRAGARIA ANANASSA ; Fruit - enzymology ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes. Genome ; Genome, Plant ; genomic DNA ; Isoenzymes - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molecular Weight ; PEROXIDASAS ; PEROXIDASES ; Peroxidases - genetics ; PEROXYDASE ; Pisum sativum - enzymology ; promoter ; Restriction Mapping ; strawberry (Fragaria x ananassa) ; Transcription, Genetic - genetics</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1998-07, Vol.62 (7), p.1358-1363</ispartof><rights>1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c639t-987e8b9d60625c721a4ff3547514ce59b54f4d3a4d20f3709bcc2cd29b324b5a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2417948$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9720218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon)</creatorcontrib><creatorcontrib>Chung, W.I</creatorcontrib><title>Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from the strawberry (Fragaria x ananassa)</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. The ApxSC genomic DNA contains 10 exons and 9 introns, which is similar to the structure of pea ApxI. A primer extension analysis suggested that the transcription of ApxSC gene was started at three s tart sites with different degrees. The promoter region of ApxSC gene contains a sequence or structure distinct from other reported plant ascorbate peroxidase genes, though with several known functional elements such as a TATA box</description><subject>ACIDE ASCORBIQUE</subject><subject>ACIDO ASCORBICO</subject><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis - enzymology</subject><subject>Ascorbate Peroxidases</subject><subject>ASCORBIC ACID</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cytosol - enzymology</subject><subject>cytosolic ascorbate peroxidase</subject><subject>DNA</subject><subject>DNA, Plant - chemistry</subject><subject>DNA, Plant - isolation & purification</subject><subject>FRAGARIA ANANASSA</subject><subject>Fruit - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes. Genome</subject><subject>Genome, Plant</subject><subject>genomic DNA</subject><subject>Isoenzymes - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>PEROXIDASAS</subject><subject>PEROXIDASES</subject><subject>Peroxidases - genetics</subject><subject>PEROXYDASE</subject><subject>Pisum sativum - enzymology</subject><subject>promoter</subject><subject>Restriction Mapping</subject><subject>strawberry (Fragaria x ananassa)</subject><subject>Transcription, Genetic - genetics</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90s-LEzEUB_BBlLWunjwLAUV2kamT35Njqa6uLCqo5_CSSeosM0lNpmyL_7wZWvfgQXII5H1eEvJNVT3HzRITid8aY5aCLDHl7YNqgSmTtVBMPqwWjcKibhnHj6snOd82TVng-Kw6U5I0BLeL6vd1jgNMfQwoerRxIY69Re8-r5CNYYI-9GGDANnDFAssJcg2JgOTQ1uX4r7vILu5z6GL1Xb_bX2JfIojmn46lKcEd8aldEAXVwk2kHpAewShjJzh8mn1yMOQ3bPTfF79uHr_ff2xvvny4Xq9uqmtoGqqVStda1QnGkG4lQQD855yJjlm1nFlOPOso8A60ngqG2WsJbYjylDCDAd6Xr0-7rtN8dfO5UmPfbZuGCC4uMta0pZzRVSBL_-Bt3GXQrmbxowpRlrFRFFvjsqmmHNyXm9TP0I6aNzoORBdAtGC6DmQol-c9tyZ0XX39pRAqb861cvLwuATBNvne0YYlorNTBxZH3xMI9zFNHR6gsMQ098e-t_zPUQNm1Tcp69YKTV_hlbQP5okrbw</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon)</creator><creator>Chung, W.I</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from the strawberry (Fragaria x ananassa)</title><author>Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon) ; Chung, W.I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c639t-987e8b9d60625c721a4ff3547514ce59b54f4d3a4d20f3709bcc2cd29b324b5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ACIDE ASCORBIQUE</topic><topic>ACIDO ASCORBICO</topic><topic>ADN</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis - enzymology</topic><topic>Ascorbate Peroxidases</topic><topic>ASCORBIC ACID</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cytosol - enzymology</topic><topic>cytosolic ascorbate peroxidase</topic><topic>DNA</topic><topic>DNA, Plant - chemistry</topic><topic>DNA, Plant - isolation & purification</topic><topic>FRAGARIA ANANASSA</topic><topic>Fruit - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes. Genome</topic><topic>Genome, Plant</topic><topic>genomic DNA</topic><topic>Isoenzymes - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>PEROXIDASAS</topic><topic>PEROXIDASES</topic><topic>Peroxidases - genetics</topic><topic>PEROXYDASE</topic><topic>Pisum sativum - enzymology</topic><topic>promoter</topic><topic>Restriction Mapping</topic><topic>strawberry (Fragaria x ananassa)</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon)</creatorcontrib><creatorcontrib>Chung, W.I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, I.J. (Korea Advanced Inst. of Science and Technology, Taejon)</au><au>Chung, W.I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from the strawberry (Fragaria x ananassa)</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>62</volume><issue>7</issue><spage>1358</spage><epage>1363</epage><pages>1358-1363</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. The ApxSC genomic DNA contains 10 exons and 9 introns, which is similar to the structure of pea ApxI. A primer extension analysis suggested that the transcription of ApxSC gene was started at three s tart sites with different degrees. The promoter region of ApxSC gene contains a sequence or structure distinct from other reported plant ascorbate peroxidase genes, though with several known functional elements such as a TATA box</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>9720218</pmid><doi>10.1271/bbb.62.1358</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1998-07, Vol.62 (7), p.1358-1363 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pubmed_primary_9720218 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | ACIDE ASCORBIQUE ACIDO ASCORBICO ADN Amino Acid Sequence Arabidopsis - enzymology Ascorbate Peroxidases ASCORBIC ACID Base Sequence Biological and medical sciences Cytosol - enzymology cytosolic ascorbate peroxidase DNA DNA, Plant - chemistry DNA, Plant - isolation & purification FRAGARIA ANANASSA Fruit - enzymology Fundamental and applied biological sciences. Psychology GENE GENES Genes. Genome Genome, Plant genomic DNA Isoenzymes - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecular Weight PEROXIDASAS PEROXIDASES Peroxidases - genetics PEROXYDASE Pisum sativum - enzymology promoter Restriction Mapping strawberry (Fragaria x ananassa) Transcription, Genetic - genetics |
| title | Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from the strawberry (Fragaria x ananassa) |
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