Generation of up-regulated allosteric variants of potato ADP-glucose pyrophosphorylase by reversion genetics

Mutagenesis of the large subunit (LS) of the potato ADP-glucose pyrophosphorylase generated an enzyme, P52L, that was insensitive to 3-phosphoglycerate (3-PGA). To identify additional residues involved in 3-PGA interaction, we subjected P52L LS DNA to a second round of mutagenesis and identified sec...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-08, Vol.95 (17), p.10322-10327
Hauptverfasser:	Greene, T.W. (Washington State University, Pullman, WA.), Kavakli, I.H, Kahn, M.L, Okita, T.W
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Allosteric regulation Allosteric Site - genetics Amino Acid Sequence Base Sequence BINDING Biological Sciences CARBOHYDRATE METABOLISM DNA Primers - genetics Enzyme Activation - drug effects Enzyme Activation - genetics ENZYME INHIBITORS Enzyme Stability - genetics Enzymes ENZYMIC ACTIVITY FOSFATOS (ESTERES) FOSFORO GENE GENES Genes, Plant Genetic mutation Genetic Variation Genetics GLICOGENO Glucose-1-Phosphate Adenylyltransferase GLYCERATE 3-PHOSPHATE Glyceric Acids - metabolism Glyceric Acids - pharmacology GLYCOGEN GLYCOGENE Hot Temperature INDUCED MUTATION INHIBIDORES DE ENZIMAS INHIBITEUR D'ENZYME KINASES Kinetics METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Molecular Sequence Data MUTACION MUTACION INDUCIDA Mutagenesis Mutagenesis, Site-Directed MUTANT MUTANTES MUTANTS MUTATION MUTATION PROVOQUEE Nucleotidyltransferases - chemistry Nucleotidyltransferases - genetics Nucleotidyltransferases - metabolism ORTHOPHOSPHATE PHOSPHATE (ESTER) Phosphates PHOSPHATES (ESTERS) PHOSPHORE PHOSPHORUS Plants Plasmids Potatoes RESISTANCE Sequence Homology, Amino Acid SOLANUM TUBEROSUM Solanum tuberosum - enzymology Solanum tuberosum - genetics Starch - biosynthesis Starches TARGETED MUTAGENESIS TRANSFERASAS TRANSFERASE TRANSFERASES Up-Regulation
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