pH-sensitive imaging by low-frequency EPR: a model study for biological applications
The use of pH-sensitive nitroxides, in conjunction with low-frequency EPR, offers a unique opportunity for non-invasive assessment of pH values (in the range 0 to 14) in living animals. In the present study, we have investigated the potential use of pH-sensitive nitroxide free radicals in conjunctio...
Gespeichert in:
| Veröffentlicht in: | Physics in medicine & biology 1998-07, Vol.43 (7), p.1921-1930 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1930 |
|---|---|
| container_issue | 7 |
| container_start_page | 1921 |
| container_title | Physics in medicine & biology |
| container_volume | 43 |
| creator | Sotgiu, Antonello Mäder, Karsten Placidi, Giuseppe Colacicchi, Silvia Ursini, Cinzia Lucia Alecci, Marcello |
| description | The use of pH-sensitive nitroxides, in conjunction with low-frequency EPR, offers a unique opportunity for non-invasive assessment of pH values (in the range 0 to 14) in living animals. In the present study, we have investigated the potential use of pH-sensitive nitroxide free radicals in conjunction with EPR imaging techniques at low and very low frequencies (280 MHz-2.1 GHz). In particular, we have measured the hyperfine splitting (hfs) of a pH-sensitive probe at three different EPR frequencies: 280 MHz, 1.1 GHz and 2.1 GHz. We have also developed EPR imaging experiments with phantoms simulating in vivo conditions, using pH-sensitive probes at 280 MHz (spatial-spatial) and 1.1 GHz (spectral-spatial). Finally, we discuss the actual sensitivity/resolution limits of the EPR imaging techniques at low frequencies. Practical applications of this method in the biomedical field are suggested for the continuous and non-invasive localization of pH in vivo. |
| doi_str_mv | 10.1088/0031-9155/43/7/013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_9703055</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73834367</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-417941cc0a3090e2183c6f554e5b3a511ff75f967307f4d80ad80e5a5e804d853</originalsourceid><addsrcrecordid>eNqNkEFLwzAUx4Moc06_gCDkJHiofVmSpvUmYzphoMg8h7RNRqRtatMq_fZmbOzixUNIwvu9_0t-CF0TuCeQpjEAJVFGOI8ZjUUMhJ6gKaEJiRKewCmaHoFzdOH9JwAh6ZxN0CQTQIHzKdq0q8jrxtvefmtsa7W1zRbnI67cT2Q6_TXophjx8u39AStcu1JX2PdDOWLjOpxbV7mtLVSFVdtW4dBb1_hLdGZU5fXVYZ-hj6flZrGK1q_PL4vHdVQwmvQRIyJjpChAUchAz0lKi8RwzjTPqeKEGCO4yRJBQRhWpqDC0lxxnUK4cjpDt_vctnPhob6XtfWFrirVaDd4KWhKwyQRwPkeLDrnfaeNbLvw126UBOROpdyZkjtTklEpZFAZmm4O6UNe6_LYcnAX6tG-bl37v7y7v_xfTralob9oRYgd</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73834367</pqid></control><display><type>article</type><title>pH-sensitive imaging by low-frequency EPR: a model study for biological applications</title><source>MEDLINE</source><source>IOP Publishing Journals</source><source>Institute of Physics (IOP) Journals - HEAL-Link</source><creator>Sotgiu, Antonello ; Mäder, Karsten ; Placidi, Giuseppe ; Colacicchi, Silvia ; Ursini, Cinzia Lucia ; Alecci, Marcello</creator><creatorcontrib>Sotgiu, Antonello ; Mäder, Karsten ; Placidi, Giuseppe ; Colacicchi, Silvia ; Ursini, Cinzia Lucia ; Alecci, Marcello</creatorcontrib><description>The use of pH-sensitive nitroxides, in conjunction with low-frequency EPR, offers a unique opportunity for non-invasive assessment of pH values (in the range 0 to 14) in living animals. In the present study, we have investigated the potential use of pH-sensitive nitroxide free radicals in conjunction with EPR imaging techniques at low and very low frequencies (280 MHz-2.1 GHz). In particular, we have measured the hyperfine splitting (hfs) of a pH-sensitive probe at three different EPR frequencies: 280 MHz, 1.1 GHz and 2.1 GHz. We have also developed EPR imaging experiments with phantoms simulating in vivo conditions, using pH-sensitive probes at 280 MHz (spatial-spatial) and 1.1 GHz (spectral-spatial). Finally, we discuss the actual sensitivity/resolution limits of the EPR imaging techniques at low frequencies. Practical applications of this method in the biomedical field are suggested for the continuous and non-invasive localization of pH in vivo.</description><identifier>ISSN: 0031-9155</identifier><identifier>EISSN: 1361-6560</identifier><identifier>DOI: 10.1088/0031-9155/43/7/013</identifier><identifier>PMID: 9703055</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Animals ; Biophysical Phenomena ; Biophysics ; Electron Spin Resonance Spectroscopy - methods ; Free Radicals - metabolism ; Hydrogen-Ion Concentration ; Models, Biological ; Nitrogen Oxides - metabolism ; Phantoms, Imaging ; Radio Waves ; Rats ; Spin Labels</subject><ispartof>Physics in medicine & biology, 1998-07, Vol.43 (7), p.1921-1930</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-417941cc0a3090e2183c6f554e5b3a511ff75f967307f4d80ad80e5a5e804d853</citedby><cites>FETCH-LOGICAL-c436t-417941cc0a3090e2183c6f554e5b3a511ff75f967307f4d80ad80e5a5e804d853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/0031-9155/43/7/013/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,776,780,27901,27902,53805,53885</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9703055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sotgiu, Antonello</creatorcontrib><creatorcontrib>Mäder, Karsten</creatorcontrib><creatorcontrib>Placidi, Giuseppe</creatorcontrib><creatorcontrib>Colacicchi, Silvia</creatorcontrib><creatorcontrib>Ursini, Cinzia Lucia</creatorcontrib><creatorcontrib>Alecci, Marcello</creatorcontrib><title>pH-sensitive imaging by low-frequency EPR: a model study for biological applications</title><title>Physics in medicine & biology</title><addtitle>Phys Med Biol</addtitle><description>The use of pH-sensitive nitroxides, in conjunction with low-frequency EPR, offers a unique opportunity for non-invasive assessment of pH values (in the range 0 to 14) in living animals. In the present study, we have investigated the potential use of pH-sensitive nitroxide free radicals in conjunction with EPR imaging techniques at low and very low frequencies (280 MHz-2.1 GHz). In particular, we have measured the hyperfine splitting (hfs) of a pH-sensitive probe at three different EPR frequencies: 280 MHz, 1.1 GHz and 2.1 GHz. We have also developed EPR imaging experiments with phantoms simulating in vivo conditions, using pH-sensitive probes at 280 MHz (spatial-spatial) and 1.1 GHz (spectral-spatial). Finally, we discuss the actual sensitivity/resolution limits of the EPR imaging techniques at low frequencies. Practical applications of this method in the biomedical field are suggested for the continuous and non-invasive localization of pH in vivo.</description><subject>Animals</subject><subject>Biophysical Phenomena</subject><subject>Biophysics</subject><subject>Electron Spin Resonance Spectroscopy - methods</subject><subject>Free Radicals - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Models, Biological</subject><subject>Nitrogen Oxides - metabolism</subject><subject>Phantoms, Imaging</subject><subject>Radio Waves</subject><subject>Rats</subject><subject>Spin Labels</subject><issn>0031-9155</issn><issn>1361-6560</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFLwzAUx4Moc06_gCDkJHiofVmSpvUmYzphoMg8h7RNRqRtatMq_fZmbOzixUNIwvu9_0t-CF0TuCeQpjEAJVFGOI8ZjUUMhJ6gKaEJiRKewCmaHoFzdOH9JwAh6ZxN0CQTQIHzKdq0q8jrxtvefmtsa7W1zRbnI67cT2Q6_TXophjx8u39AStcu1JX2PdDOWLjOpxbV7mtLVSFVdtW4dBb1_hLdGZU5fXVYZ-hj6flZrGK1q_PL4vHdVQwmvQRIyJjpChAUchAz0lKi8RwzjTPqeKEGCO4yRJBQRhWpqDC0lxxnUK4cjpDt_vctnPhob6XtfWFrirVaDd4KWhKwyQRwPkeLDrnfaeNbLvw126UBOROpdyZkjtTklEpZFAZmm4O6UNe6_LYcnAX6tG-bl37v7y7v_xfTralob9oRYgd</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Sotgiu, Antonello</creator><creator>Mäder, Karsten</creator><creator>Placidi, Giuseppe</creator><creator>Colacicchi, Silvia</creator><creator>Ursini, Cinzia Lucia</creator><creator>Alecci, Marcello</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>pH-sensitive imaging by low-frequency EPR: a model study for biological applications</title><author>Sotgiu, Antonello ; Mäder, Karsten ; Placidi, Giuseppe ; Colacicchi, Silvia ; Ursini, Cinzia Lucia ; Alecci, Marcello</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-417941cc0a3090e2183c6f554e5b3a511ff75f967307f4d80ad80e5a5e804d853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biophysical Phenomena</topic><topic>Biophysics</topic><topic>Electron Spin Resonance Spectroscopy - methods</topic><topic>Free Radicals - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Models, Biological</topic><topic>Nitrogen Oxides - metabolism</topic><topic>Phantoms, Imaging</topic><topic>Radio Waves</topic><topic>Rats</topic><topic>Spin Labels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sotgiu, Antonello</creatorcontrib><creatorcontrib>Mäder, Karsten</creatorcontrib><creatorcontrib>Placidi, Giuseppe</creatorcontrib><creatorcontrib>Colacicchi, Silvia</creatorcontrib><creatorcontrib>Ursini, Cinzia Lucia</creatorcontrib><creatorcontrib>Alecci, Marcello</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Physics in medicine & biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sotgiu, Antonello</au><au>Mäder, Karsten</au><au>Placidi, Giuseppe</au><au>Colacicchi, Silvia</au><au>Ursini, Cinzia Lucia</au><au>Alecci, Marcello</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pH-sensitive imaging by low-frequency EPR: a model study for biological applications</atitle><jtitle>Physics in medicine & biology</jtitle><addtitle>Phys Med Biol</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>43</volume><issue>7</issue><spage>1921</spage><epage>1930</epage><pages>1921-1930</pages><issn>0031-9155</issn><eissn>1361-6560</eissn><abstract>The use of pH-sensitive nitroxides, in conjunction with low-frequency EPR, offers a unique opportunity for non-invasive assessment of pH values (in the range 0 to 14) in living animals. In the present study, we have investigated the potential use of pH-sensitive nitroxide free radicals in conjunction with EPR imaging techniques at low and very low frequencies (280 MHz-2.1 GHz). In particular, we have measured the hyperfine splitting (hfs) of a pH-sensitive probe at three different EPR frequencies: 280 MHz, 1.1 GHz and 2.1 GHz. We have also developed EPR imaging experiments with phantoms simulating in vivo conditions, using pH-sensitive probes at 280 MHz (spatial-spatial) and 1.1 GHz (spectral-spatial). Finally, we discuss the actual sensitivity/resolution limits of the EPR imaging techniques at low frequencies. Practical applications of this method in the biomedical field are suggested for the continuous and non-invasive localization of pH in vivo.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>9703055</pmid><doi>10.1088/0031-9155/43/7/013</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9155 |
| ispartof | Physics in medicine & biology, 1998-07, Vol.43 (7), p.1921-1930 |
| issn | 0031-9155 1361-6560 |
| language | eng |
| recordid | cdi_pubmed_primary_9703055 |
| source | MEDLINE; IOP Publishing Journals; Institute of Physics (IOP) Journals - HEAL-Link |
| subjects | Animals Biophysical Phenomena Biophysics Electron Spin Resonance Spectroscopy - methods Free Radicals - metabolism Hydrogen-Ion Concentration Models, Biological Nitrogen Oxides - metabolism Phantoms, Imaging Radio Waves Rats Spin Labels |
| title | pH-sensitive imaging by low-frequency EPR: a model study for biological applications |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T14%3A28%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=pH-sensitive%20imaging%20by%20low-frequency%20EPR:%20a%20model%20study%20for%20biological%20applications&rft.jtitle=Physics%20in%20medicine%20&%20biology&rft.au=Sotgiu,%20Antonello&rft.date=1998-07-01&rft.volume=43&rft.issue=7&rft.spage=1921&rft.epage=1930&rft.pages=1921-1930&rft.issn=0031-9155&rft.eissn=1361-6560&rft_id=info:doi/10.1088/0031-9155/43/7/013&rft_dat=%3Cproquest_pubme%3E73834367%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73834367&rft_id=info:pmid/9703055&rfr_iscdi=true |