Pathways of DNA repair in T4 phage: II. Sedimentation analysis of intracellular DNA in repair-defective mutants
The function of various pathways of repair was assessed by sucrose gradient centrifugation of uv-irradiated intracellular T4 DNA. Mutation in the v gene inhibits the excision repair pathway and results in a slower endonucleolytic reaction, a delay in “joining” of fragments and a decreased rate of DN...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1976, Vol.73 (1), p.155-164 |
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| container_issue | 1 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 73 |
| creator | Wakem, L.P. Ebisuzaki, K. |
| description | The function of various pathways of repair was assessed by sucrose gradient centrifugation of uv-irradiated intracellular T4 DNA. Mutation in the
v gene inhibits the excision repair pathway and results in a slower endonucleolytic reaction, a delay in “joining” of fragments and a decreased rate of DNA replication following uv irradiation. Mutations in the
x or
y genes inhibit an alternate pathway and result in the failure to “join” single-strand breaks after irradiation. In the absence of uv irradiation, the
x and
y mutations lead to the accumulation of DNA with single-strand breaks and a decrease in the amount of concatemers. We postulate that the
x and
y genes are involved in a “joining” reaction involving DNA fragments. |
| doi_str_mv | 10.1016/0042-6822(76)90069-6 |
| format | Article |
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v gene inhibits the excision repair pathway and results in a slower endonucleolytic reaction, a delay in “joining” of fragments and a decreased rate of DNA replication following uv irradiation. Mutations in the
x or
y genes inhibit an alternate pathway and result in the failure to “join” single-strand breaks after irradiation. In the absence of uv irradiation, the
x and
y mutations lead to the accumulation of DNA with single-strand breaks and a decrease in the amount of concatemers. We postulate that the
x and
y genes are involved in a “joining” reaction involving DNA fragments.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(76)90069-6</identifier><identifier>PMID: 960557</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987) ; BACTERIOPHAGES ; BIOLOGICAL RECOVERY ; BIOLOGICAL REPAIR ; Coliphages - metabolism ; Coliphages - radiation effects ; DNA ; DNA Repair ; DNA REPLICATION ; DNA, Viral - biosynthesis ; DNA, Viral - radiation effects ; ELECTROMAGNETIC RADIATION ; Genes ; IRRADIATION ; MICROORGANISMS ; Mutation ; MUTATIONS ; NUCLEIC ACID REPLICATION ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; PARASITES ; Radiation Effects ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RECOVERY ; REPAIR ; ULTRAVIOLET RADIATION ; Ultraviolet Rays ; VIRUSES</subject><ispartof>Virology (New York, N.Y.), 1976, Vol.73 (1), p.155-164</ispartof><rights>1976</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(76)90069-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,885,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/960557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7325867$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakem, L.P.</creatorcontrib><creatorcontrib>Ebisuzaki, K.</creatorcontrib><creatorcontrib>Univ. of Western Ontario, London</creatorcontrib><title>Pathways of DNA repair in T4 phage: II. Sedimentation analysis of intracellular DNA in repair-defective mutants</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The function of various pathways of repair was assessed by sucrose gradient centrifugation of uv-irradiated intracellular T4 DNA. Mutation in the
v gene inhibits the excision repair pathway and results in a slower endonucleolytic reaction, a delay in “joining” of fragments and a decreased rate of DNA replication following uv irradiation. Mutations in the
x or
y genes inhibit an alternate pathway and result in the failure to “join” single-strand breaks after irradiation. In the absence of uv irradiation, the
x and
y mutations lead to the accumulation of DNA with single-strand breaks and a decrease in the amount of concatemers. We postulate that the
x and
y genes are involved in a “joining” reaction involving DNA fragments.</description><subject>560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)</subject><subject>BACTERIOPHAGES</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>Coliphages - metabolism</subject><subject>Coliphages - radiation effects</subject><subject>DNA</subject><subject>DNA Repair</subject><subject>DNA REPLICATION</subject><subject>DNA, Viral - biosynthesis</subject><subject>DNA, Viral - radiation effects</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>Genes</subject><subject>IRRADIATION</subject><subject>MICROORGANISMS</subject><subject>Mutation</subject><subject>MUTATIONS</subject><subject>NUCLEIC ACID REPLICATION</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>PARASITES</subject><subject>Radiation Effects</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RECOVERY</subject><subject>REPAIR</subject><subject>ULTRAVIOLET RADIATION</subject><subject>Ultraviolet Rays</subject><subject>VIRUSES</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtPwzAQhC3EqxT-AQeLExxS1nZiJxyQqvKqVAES5WxtHYcapU6VuEX99yQN4rQa7eyn0SwhlwxGDJi8BYh5JFPOr5W8yQBkFskDMmCQyQhEzA7J4N9ySs6a5htarRSckONMQpKoAaneMSx_cNfQqqAPr2Na2zW6mjpP5zFdL_HL3tHpdEQ_bO5W1gcMrvIUPZa7xu2vnA81GluWmxLrPaM97jFRbgtrgttautoE9KE5J0cFlo29-JtD8vn0OJ-8RLO35-lkPIssBx4ixCQ1hUgLYxdgFKLMAZlKgLfSxDwVWRILAQK4ZJkBgVmuijhNEwVsIbgYkqueWzXB6ca4YM3SVN63cbQSPEmlak2XvWm9Waxsrte1W2G903057fq-X9s26NbZuuNYb9om6g6TV04z0N0vdFe07orWSur9L7QUv6_jeXQ</recordid><startdate>1976</startdate><enddate>1976</enddate><creator>Wakem, L.P.</creator><creator>Ebisuzaki, K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>OTOTI</scope></search><sort><creationdate>1976</creationdate><title>Pathways of DNA repair in T4 phage: II. Sedimentation analysis of intracellular DNA in repair-defective mutants</title><author>Wakem, L.P. ; Ebisuzaki, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e202t-aa58cf38fceb0c7aa6d0a17502b0cc4283954330302619c03a9d7f4885701b323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)</topic><topic>BACTERIOPHAGES</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>Coliphages - metabolism</topic><topic>Coliphages - radiation effects</topic><topic>DNA</topic><topic>DNA Repair</topic><topic>DNA REPLICATION</topic><topic>DNA, Viral - biosynthesis</topic><topic>DNA, Viral - radiation effects</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>Genes</topic><topic>IRRADIATION</topic><topic>MICROORGANISMS</topic><topic>Mutation</topic><topic>MUTATIONS</topic><topic>NUCLEIC ACID REPLICATION</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>PARASITES</topic><topic>Radiation Effects</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>RECOVERY</topic><topic>REPAIR</topic><topic>ULTRAVIOLET RADIATION</topic><topic>Ultraviolet Rays</topic><topic>VIRUSES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wakem, L.P.</creatorcontrib><creatorcontrib>Ebisuzaki, K.</creatorcontrib><creatorcontrib>Univ. of Western Ontario, London</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>OSTI.GOV</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakem, L.P.</au><au>Ebisuzaki, K.</au><aucorp>Univ. of Western Ontario, London</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathways of DNA repair in T4 phage: II. Sedimentation analysis of intracellular DNA in repair-defective mutants</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1976</date><risdate>1976</risdate><volume>73</volume><issue>1</issue><spage>155</spage><epage>164</epage><pages>155-164</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The function of various pathways of repair was assessed by sucrose gradient centrifugation of uv-irradiated intracellular T4 DNA. Mutation in the
v gene inhibits the excision repair pathway and results in a slower endonucleolytic reaction, a delay in “joining” of fragments and a decreased rate of DNA replication following uv irradiation. Mutations in the
x or
y genes inhibit an alternate pathway and result in the failure to “join” single-strand breaks after irradiation. In the absence of uv irradiation, the
x and
y mutations lead to the accumulation of DNA with single-strand breaks and a decrease in the amount of concatemers. We postulate that the
x and
y genes are involved in a “joining” reaction involving DNA fragments.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>960557</pmid><doi>10.1016/0042-6822(76)90069-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1976, Vol.73 (1), p.155-164 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_pubmed_primary_960557 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier) |
| subjects | 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987) BACTERIOPHAGES BIOLOGICAL RECOVERY BIOLOGICAL REPAIR Coliphages - metabolism Coliphages - radiation effects DNA DNA Repair DNA REPLICATION DNA, Viral - biosynthesis DNA, Viral - radiation effects ELECTROMAGNETIC RADIATION Genes IRRADIATION MICROORGANISMS Mutation MUTATIONS NUCLEIC ACID REPLICATION NUCLEIC ACIDS ORGANIC COMPOUNDS PARASITES Radiation Effects RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RECOVERY REPAIR ULTRAVIOLET RADIATION Ultraviolet Rays VIRUSES |
| title | Pathways of DNA repair in T4 phage: II. Sedimentation analysis of intracellular DNA in repair-defective mutants |
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