[6] Molecular approaches to identifying ligand binding and signaling domains of C-C chemokine receptors
The construction of chimeric receptors provides a useful starting point for the identification of ligand-binding and G-protein-coupling sites on chemokine receptors. Correlation of the binding and signaling properties of a set of complementary receptor chimeras is a powerful approach for probing str...
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| Veröffentlicht in: | Methods in Enzymology 1997, Vol.288, p.70-84 |
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| container_title | Methods in Enzymology |
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| creator | Monteclaro, Felipe S. Arai, Hidenori Chard, Israel F. |
| description | The construction of chimeric receptors provides a useful starting point for the identification of ligand-binding and G-protein-coupling sites on chemokine receptors. Correlation of the binding and signaling properties of a set of complementary receptor chimeras is a powerful approach for probing structure-function relationships. Further molecular resolution can subsequently be achieved by site-directed mutagenesis and/or alanine scanning. |
| doi_str_mv | 10.1016/S0076-6879(97)88008-1 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0076-6879 |
| ispartof | Methods in Enzymology, 1997, Vol.288, p.70-84 |
| issn | 0076-6879 1557-7988 |
| language | eng |
| recordid | cdi_pubmed_primary_9356988 |
| source | ScienceDirect eBooks; MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Binding Sites Binding, Competitive Cell Line Cell Membrane - metabolism GTP-Binding Proteins - metabolism Humans Inositol Phosphates - metabolism Ligands Models, Molecular Molecular Sequence Data Polymerase Chain Reaction Protein Binding Receptors, CCR1 Receptors, CCR2 Receptors, Chemokine - chemistry Receptors, Chemokine - genetics Receptors, Chemokine - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Sequence Alignment Signal Transduction - physiology Substrate Specificity Transfection |
| title | [6] Molecular approaches to identifying ligand binding and signaling domains of C-C chemokine receptors |
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