Keratinocyte Growth Factor and Receptor mRNA Expression in Cholesteatoma of the Middle Ear
Keratinocyte growth factor (KGF) is synthesized and secreted exclusively by stromal cells in epithelialized organs, and specifically promotes proliferation of cells of epithelial origin, including keratinocytes. Cholesteatoma is a proliferative form of keratinocyte dysregulation with aggressive grow...
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| Veröffentlicht in: | Acta oto-laryngologica 1997, Vol.117 (5), p.714-718 |
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| creator | Ishibashi, Toshio Shinogami, Masanobu Kaga, Kimitaka Fukaya, Takashi |
| description | Keratinocyte growth factor (KGF) is synthesized and secreted exclusively by stromal cells in epithelialized organs, and specifically promotes proliferation of cells of epithelial origin, including keratinocytes. Cholesteatoma is a proliferative form of keratinocyte dysregulation with aggressive growth that often leads to destruction of adjacent bone. We examined the expression of KGF, closely related peptides and their receptors in the development of cholesteatoma by comparing cholesteatoma with normal ear skin using the reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. All surgical specimens of cholesteatoma and most normal ear skin samples expressed detectable levels of KGF and KGF receptor (KGFR). Semiquantitative RT-PCR using β-actin mRNA as an internal standard revealed significantly higher expression of KGF nvRNA in cholesteatoma than in normal skin, while no significant difference in KGFR mRNA expression was noted between cholesteatoma tissue and normal skin. These results suggest that excessive KGF synthesis may contribute to the hyperproliferative state in cholesteatoma. |
| doi_str_mv | 10.3109/00016489709113465 |
| format | Article |
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Cholesteatoma is a proliferative form of keratinocyte dysregulation with aggressive growth that often leads to destruction of adjacent bone. We examined the expression of KGF, closely related peptides and their receptors in the development of cholesteatoma by comparing cholesteatoma with normal ear skin using the reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. All surgical specimens of cholesteatoma and most normal ear skin samples expressed detectable levels of KGF and KGF receptor (KGFR). Semiquantitative RT-PCR using β-actin mRNA as an internal standard revealed significantly higher expression of KGF nvRNA in cholesteatoma than in normal skin, while no significant difference in KGFR mRNA expression was noted between cholesteatoma tissue and normal skin. These results suggest that excessive KGF synthesis may contribute to the hyperproliferative state in cholesteatoma.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.3109/00016489709113465</identifier><identifier>PMID: 9349868</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>Biological and medical sciences ; Biotechnology ; Blotting, Southern ; Cholesteatoma ; Cholesteatoma, Middle Ear - metabolism ; Fibroblast Growth Factor 10 ; Fibroblast Growth Factor 7 ; Fibroblast Growth Factors ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Growth Substances - biosynthesis ; Humans ; In vitro gene amplification. Pcr technique ; Keratinocytes - metabolism ; KGF ; KGF receptor ; Methods. Procedures. Technologies ; mRNA ; Polymerase Chain Reaction ; Receptor, Fibroblast Growth Factor, Type 2 ; Receptors, Fibroblast Growth Factor ; Receptors, Growth Factor - biosynthesis ; RNA, Messenger - biosynthesis ; RT-PCR ; Transcription, Genetic</subject><ispartof>Acta oto-laryngologica, 1997, Vol.117 (5), p.714-718</ispartof><rights>1997 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-4c458451ea5210c150e7f911d736ad58d57816eeeeb66b407037b1e58de7f3df3</citedby><cites>FETCH-LOGICAL-c496t-4c458451ea5210c150e7f911d736ad58d57816eeeeb66b407037b1e58de7f3df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00016489709113465$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00016489709113465$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,777,781,4010,27904,27905,27906,59626,59732,60415,60521,61200,61235,61381,61416</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2818505$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9349868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishibashi, Toshio</creatorcontrib><creatorcontrib>Shinogami, Masanobu</creatorcontrib><creatorcontrib>Kaga, Kimitaka</creatorcontrib><creatorcontrib>Fukaya, Takashi</creatorcontrib><title>Keratinocyte Growth Factor and Receptor mRNA Expression in Cholesteatoma of the Middle Ear</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Keratinocyte growth factor (KGF) is synthesized and secreted exclusively by stromal cells in epithelialized organs, and specifically promotes proliferation of cells of epithelial origin, including keratinocytes. Cholesteatoma is a proliferative form of keratinocyte dysregulation with aggressive growth that often leads to destruction of adjacent bone. We examined the expression of KGF, closely related peptides and their receptors in the development of cholesteatoma by comparing cholesteatoma with normal ear skin using the reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. All surgical specimens of cholesteatoma and most normal ear skin samples expressed detectable levels of KGF and KGF receptor (KGFR). Semiquantitative RT-PCR using β-actin mRNA as an internal standard revealed significantly higher expression of KGF nvRNA in cholesteatoma than in normal skin, while no significant difference in KGFR mRNA expression was noted between cholesteatoma tissue and normal skin. These results suggest that excessive KGF synthesis may contribute to the hyperproliferative state in cholesteatoma.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>Cholesteatoma</subject><subject>Cholesteatoma, Middle Ear - metabolism</subject><subject>Fibroblast Growth Factor 10</subject><subject>Fibroblast Growth Factor 7</subject><subject>Fibroblast Growth Factors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Growth Substances - biosynthesis</subject><subject>Humans</subject><subject>In vitro gene amplification. Pcr technique</subject><subject>Keratinocytes - metabolism</subject><subject>KGF</subject><subject>KGF receptor</subject><subject>Methods. Procedures. Technologies</subject><subject>mRNA</subject><subject>Polymerase Chain Reaction</subject><subject>Receptor, Fibroblast Growth Factor, Type 2</subject><subject>Receptors, Fibroblast Growth Factor</subject><subject>Receptors, Growth Factor - biosynthesis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RT-PCR</subject><subject>Transcription, Genetic</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFr3DAQhUVpSbdpf0APBR1Kb24ky5JlkktYNmlJmkBoL72YWWmMHWRrI2lJ999XZreBUIguYnjfe8w8Qj5y9lVw1pwwxriqdFOzhnNRKfmKLLiSvChLyV-TxawXM_CWvIvxfh4bLY_IUSOqRiu9IL-vMEAaJm92Cell8I-ppxdgkg8UJkvv0OBmHsa7m3O6-rMJGOPgJzpMdNl7hzEhJD8C9R1NPdIfg7UO6QrCe_KmAxfxw-E_Jr8uVj-X34rr28vvy_PrwlSNSkVlKqkryRFkyZnhkmHd5XNsLRRYqa2sNVeY31qpdcVqJuo1xyxkTthOHJMv-9xN8A_bvFA7DtGgczCh38a2boSSpeIZ5HvQBB9jwK7dhGGEsGs5a-c-2__6zJ5Ph_DtekT75DgUmPXPBx2iAdcFmMwQn7BScy3ZHHO2x4ap82GERx-cbRPsnA__POKlLU6f2XsEl3oDAdt7vw1TrveFG_4Cqu-hew</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Ishibashi, Toshio</creator><creator>Shinogami, Masanobu</creator><creator>Kaga, Kimitaka</creator><creator>Fukaya, Takashi</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>1997</creationdate><title>Keratinocyte Growth Factor and Receptor mRNA Expression in Cholesteatoma of the Middle Ear</title><author>Ishibashi, Toshio ; Shinogami, Masanobu ; Kaga, Kimitaka ; Fukaya, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-4c458451ea5210c150e7f911d736ad58d57816eeeeb66b407037b1e58de7f3df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>Cholesteatoma</topic><topic>Cholesteatoma, Middle Ear - metabolism</topic><topic>Fibroblast Growth Factor 10</topic><topic>Fibroblast Growth Factor 7</topic><topic>Fibroblast Growth Factors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Growth Substances - biosynthesis</topic><topic>Humans</topic><topic>In vitro gene amplification. Pcr technique</topic><topic>Keratinocytes - metabolism</topic><topic>KGF</topic><topic>KGF receptor</topic><topic>Methods. Procedures. Technologies</topic><topic>mRNA</topic><topic>Polymerase Chain Reaction</topic><topic>Receptor, Fibroblast Growth Factor, Type 2</topic><topic>Receptors, Fibroblast Growth Factor</topic><topic>Receptors, Growth Factor - biosynthesis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RT-PCR</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishibashi, Toshio</creatorcontrib><creatorcontrib>Shinogami, Masanobu</creatorcontrib><creatorcontrib>Kaga, Kimitaka</creatorcontrib><creatorcontrib>Fukaya, Takashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Acta oto-laryngologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishibashi, Toshio</au><au>Shinogami, Masanobu</au><au>Kaga, Kimitaka</au><au>Fukaya, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Keratinocyte Growth Factor and Receptor mRNA Expression in Cholesteatoma of the Middle Ear</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>1997</date><risdate>1997</risdate><volume>117</volume><issue>5</issue><spage>714</spage><epage>718</epage><pages>714-718</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>Keratinocyte growth factor (KGF) is synthesized and secreted exclusively by stromal cells in epithelialized organs, and specifically promotes proliferation of cells of epithelial origin, including keratinocytes. Cholesteatoma is a proliferative form of keratinocyte dysregulation with aggressive growth that often leads to destruction of adjacent bone. We examined the expression of KGF, closely related peptides and their receptors in the development of cholesteatoma by comparing cholesteatoma with normal ear skin using the reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. All surgical specimens of cholesteatoma and most normal ear skin samples expressed detectable levels of KGF and KGF receptor (KGFR). Semiquantitative RT-PCR using β-actin mRNA as an internal standard revealed significantly higher expression of KGF nvRNA in cholesteatoma than in normal skin, while no significant difference in KGFR mRNA expression was noted between cholesteatoma tissue and normal skin. These results suggest that excessive KGF synthesis may contribute to the hyperproliferative state in cholesteatoma.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>9349868</pmid><doi>10.3109/00016489709113465</doi><tpages>5</tpages></addata></record> |
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| subjects | Biological and medical sciences Biotechnology Blotting, Southern Cholesteatoma Cholesteatoma, Middle Ear - metabolism Fibroblast Growth Factor 10 Fibroblast Growth Factor 7 Fibroblast Growth Factors Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Growth Substances - biosynthesis Humans In vitro gene amplification. Pcr technique Keratinocytes - metabolism KGF KGF receptor Methods. Procedures. Technologies mRNA Polymerase Chain Reaction Receptor, Fibroblast Growth Factor, Type 2 Receptors, Fibroblast Growth Factor Receptors, Growth Factor - biosynthesis RNA, Messenger - biosynthesis RT-PCR Transcription, Genetic |
| title | Keratinocyte Growth Factor and Receptor mRNA Expression in Cholesteatoma of the Middle Ear |
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