Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase

The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level...

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Veröffentlicht in:	Journal of Bacteriology 1997-09, Vol.179 (18), p.5963-5966
Hauptverfasser:	Zheng, L, White, R.H, Dean, D.R
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE AZOTOBACTER VINELANDII Azotobacter vinelandii - enzymology Azotobacter vinelandii - genetics Azotobacter vinelandii - metabolism Bacteriology Biochemistry BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Dimerization ENZYMIC ACTIVITY Escherichia coli Gas Chromatography-Mass Spectrometry Genes LACTONAS LACTONE LACTONES LIASAS LYASE LYASES OXO-ACID-LYASES Oxo-Acid-Lyases - genetics Oxo-Acid-Lyases - isolation & purification Oxo-Acid-Lyases - metabolism PURIFICACION PURIFICATION Substrate Specificity
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