Over-expression of plant 14-3-3 proteins in tobacco: enhancement of the plasmalemma K + conductance of mesophyll cells

Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed t...

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Veröffentlicht in:	FEBS letters 1997-08, Vol.413 (2), p.294-298
Hauptverfasser:	Saalbach, Gerhard, Schwerdel, Marc, Natura, Gabriel, Buschmann, Peter, Christov, Veselin, Dahse, Ingo
Format:	Artikel
Sprache:	eng
Schlagworte:	14-3-3 Protein 14-3-3 Proteins Amino Acid Sequence Cell Membrane - metabolism Cloning, Molecular Covalent modification Fabaceae - genetics Gene Expression Ion channel activation Ion Transport Molecular Sequence Data Molecular Weight Nicotiana - genetics Nicotiana - metabolism Patch-Clamp Techniques Plants, Medicinal Plants, Toxic Potassium Channels - physiology Protein Biosynthesis Proteins - chemistry Proteins - genetics Proteins - physiology Protoplasts - metabolism Recombinant Fusion Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Signal transduction Tobacco transformation Tyrosine 3-Monooxygenase Vicia faba cotyledon
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