Over-expression of plant 14-3-3 proteins in tobacco: enhancement of the plasmalemma K + conductance of mesophyll cells
Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed t...
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| Veröffentlicht in: | FEBS letters 1997-08, Vol.413 (2), p.294-298 |
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| creator | Saalbach, Gerhard Schwerdel, Marc Natura, Gabriel Buschmann, Peter Christov, Veselin Dahse, Ingo |
| description | Two cDNA clones encoding 14-3-3 homologous proteins were isolated from
Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different forms of ca. 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins. These forms were mainly present in the microsomal fraction. Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K
+ conductance compared to the wild type. This indicates the involvement of 14-3-3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel. |
| doi_str_mv | 10.1016/S0014-5793(97)00865-X |
| format | Article |
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Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different forms of ca. 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins. These forms were mainly present in the microsomal fraction. Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K
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Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different forms of ca. 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins. These forms were mainly present in the microsomal fraction. Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K
+ conductance compared to the wild type. This indicates the involvement of 14-3-3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel.</description><subject>14-3-3 Protein</subject><subject>14-3-3 Proteins</subject><subject>Amino Acid Sequence</subject><subject>Cell Membrane - metabolism</subject><subject>Cloning, Molecular</subject><subject>Covalent modification</subject><subject>Fabaceae - genetics</subject><subject>Gene Expression</subject><subject>Ion channel activation</subject><subject>Ion Transport</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Patch-Clamp Techniques</subject><subject>Plants, Medicinal</subject><subject>Plants, Toxic</subject><subject>Potassium Channels - physiology</subject><subject>Protein Biosynthesis</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Proteins - physiology</subject><subject>Protoplasts - metabolism</subject><subject>Recombinant Fusion Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal transduction</subject><subject>Tobacco transformation</subject><subject>Tyrosine 3-Monooxygenase</subject><subject>Vicia faba cotyledon</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LAzEQxYMotVb_hEKOiqxOkt1k14tI8QsLPdhDbyGbTOjKfrFZi_3v3bXF0zDMe483P0LmDO4YMHn_CcDiKFGZuM7UDUAqk2hzQqYsVSISsUxPyfRfck4uQviCYU9ZNiGTjKcgAKZkt9phF-FP22EIRVPTxtO2NHVPB-eQQ9uu6bGoAy1q2je5sbZ5oFhvTW2xwkE3GPotjqZQmRKrytAPekttU7tv24-yUVJhaNrtviypxbIMl-TMmzLg1XHOyPrleb14i5ar1_fF0zJCLnkfcZBprFxiGBcKvOcClU9zk4PwwkEskTPMFEu8UyAkZwZzkCJ3NvFJzMSMzA-x7XdeodNtV1Sm2-vj-8P98XDHocOuwE4HW-BQ2RUd2l67ptAM9Mhb__HWI0ydKf3HW2_ELxd_cc4</recordid><startdate>19970818</startdate><enddate>19970818</enddate><creator>Saalbach, Gerhard</creator><creator>Schwerdel, Marc</creator><creator>Natura, Gabriel</creator><creator>Buschmann, Peter</creator><creator>Christov, Veselin</creator><creator>Dahse, Ingo</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19970818</creationdate><title>Over-expression of plant 14-3-3 proteins in tobacco: enhancement of the plasmalemma K + conductance of mesophyll cells</title><author>Saalbach, Gerhard ; 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Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different forms of ca. 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins. These forms were mainly present in the microsomal fraction. Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K
+ conductance compared to the wild type. This indicates the involvement of 14-3-3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9280300</pmid><doi>10.1016/S0014-5793(97)00865-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 14-3-3 Protein 14-3-3 Proteins Amino Acid Sequence Cell Membrane - metabolism Cloning, Molecular Covalent modification Fabaceae - genetics Gene Expression Ion channel activation Ion Transport Molecular Sequence Data Molecular Weight Nicotiana - genetics Nicotiana - metabolism Patch-Clamp Techniques Plants, Medicinal Plants, Toxic Potassium Channels - physiology Protein Biosynthesis Proteins - chemistry Proteins - genetics Proteins - physiology Protoplasts - metabolism Recombinant Fusion Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Signal transduction Tobacco transformation Tyrosine 3-Monooxygenase Vicia faba cotyledon |
| title | Over-expression of plant 14-3-3 proteins in tobacco: enhancement of the plasmalemma K + conductance of mesophyll cells |
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