Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae

A genomic DNA segment encoding an extracellular laccase was isolated from the thermophilic fungus Myceliophthora thermophila, and the nucleotide sequence of this gene was determined. The deduced amino acid sequence of M. thermophila laccase (MtL) shows homology to laccases from diverse fungal genera...

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Veröffentlicht in:	Applied and Environmental Microbiology 1997-08, Vol.63 (8), p.3151-3157
Hauptverfasser:	Berka, R.M. (Novo Nordisk Biotech, Inc., Davis, CA.), Schneider, P, Golightly, E.J, Brown, S.H, Madden, M, Brown, K.M, Halkier, T, Mondorf, K, Xu, F
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - genetics ASPERGILLUS ORYZAE Aspergillus oryzae - enzymology Aspergillus oryzae - genetics Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Chromatography, Gel Chromatography, Ion Exchange Cloning Cloning, Molecular Codon, Terminator Deoxyribonucleic acid DNA DNA, Fungal - genetics Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology GENBANK/T10922 Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Gene Library Genes Genetic engineering Genetics Hydrazones - metabolism Laccase Mission oriented research Mitosporic Fungi - enzymology Mitosporic Fungi - genetics MOLECULAR SEQUENCE DATA Myceliophthora thermophila NUCLEOTIDE SEQUENCE Oxidoreductases - genetics Oxidoreductases - isolation & purification Oxidoreductases - metabolism Plasmids Promoter Regions, Genetic Recombination, Genetic SECUENCIA NUCLEOTIDICA Sequence Analysis, DNA Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE Transcription, Genetic
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