Calcium transport in microsomes isolated from rat parotid gland. Effects of ADP and an ATP-regenerating system
Calcium loading of a rat parotid microsomal fraction is greatly increased by an ATP-regenerating system (phosphocreatine and creatine phosphokinase). This effect is neither a consequence of a rise in the ATP concentration nor of an increased formation of inorganic phosphate originating from hydrolys...
Gespeichert in:
| Veröffentlicht in: | Archives of physiology and biochemistry 1996-12, Vol.104 (7), p.826 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | fre |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 7 |
| container_start_page | 826 |
| container_title | Archives of physiology and biochemistry |
| container_volume | 104 |
| creator | Eboué, D Sezan, A Rossignol, B |
| description | Calcium loading of a rat parotid microsomal fraction is greatly increased by an ATP-regenerating system (phosphocreatine and creatine phosphokinase). This effect is neither a consequence of a rise in the ATP concentration nor of an increased formation of inorganic phosphate originating from hydrolysis of ATP or phosphocreatine. Addition of ADP to the incubation medium provokes an inhibition of Ca2+ influx and a stimulation of Ca2+ efflux by the microsomal fraction. These results suggest that the stimulation of Ca2+ uptake by the ATP-regenerating system is due, at least in part, to an increase of Ca2+ influx and a slowing down of Ca2+ efflux as consequence of a decrease of ADP availability. It is proposed that the effect of ADP on Ca2+ movements could account for the action of certain agonists on intracellular Ca2+ concentration. Moreover, the InsP3 responsive Ca2+ pool was also shown to be enlarged by the ATP-regenerating system without modification of InsP3 sensitivity. |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_9221105</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>9221105</sourcerecordid><originalsourceid>FETCH-pubmed_primary_92211053</originalsourceid><addsrcrecordid>eNqFjr0OgjAUhTtoEH8eweS-AIaCJDAS1Dg6uJsKt6SGtuTeMvj2MrA7nJzkO_mSsxKxzEuZ5Oei2Igt8ydNZVZWMhJRlWVSpkUsXKOG1kwWAinHo6cAxoE1LXn2FhkM-0EF7ECTt0AqwKjIB9NBPyjXneCqNbaBwWuoLw-Y2Ryon4-EsEeHs2JcD_zlgHYv1loNjIeld-J4uz6bezJOb4vdayRjFX1fy7_83_4DVQVGJA</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Calcium transport in microsomes isolated from rat parotid gland. Effects of ADP and an ATP-regenerating system</title><source>MEDLINE</source><source>Taylor & Francis Medical Library - CRKN</source><source>Taylor & Francis Journals Complete</source><creator>Eboué, D ; Sezan, A ; Rossignol, B</creator><creatorcontrib>Eboué, D ; Sezan, A ; Rossignol, B</creatorcontrib><description>Calcium loading of a rat parotid microsomal fraction is greatly increased by an ATP-regenerating system (phosphocreatine and creatine phosphokinase). This effect is neither a consequence of a rise in the ATP concentration nor of an increased formation of inorganic phosphate originating from hydrolysis of ATP or phosphocreatine. Addition of ADP to the incubation medium provokes an inhibition of Ca2+ influx and a stimulation of Ca2+ efflux by the microsomal fraction. These results suggest that the stimulation of Ca2+ uptake by the ATP-regenerating system is due, at least in part, to an increase of Ca2+ influx and a slowing down of Ca2+ efflux as consequence of a decrease of ADP availability. It is proposed that the effect of ADP on Ca2+ movements could account for the action of certain agonists on intracellular Ca2+ concentration. Moreover, the InsP3 responsive Ca2+ pool was also shown to be enlarged by the ATP-regenerating system without modification of InsP3 sensitivity.</description><identifier>ISSN: 1381-3455</identifier><identifier>PMID: 9221105</identifier><language>fre</language><publisher>England</publisher><subject>Adenosine Diphosphate - pharmacology ; Adenosine Triphosphatases - metabolism ; Adenosine Triphosphate - metabolism ; Animals ; Biological Transport ; Calcium - metabolism ; Calcium Radioisotopes - metabolism ; Creatine Kinase - metabolism ; Inositol 1,4,5-Trisphosphate - metabolism ; Ionomycin - pharmacology ; Ionophores - pharmacology ; Male ; Microsomes - metabolism ; Parotid Gland - metabolism ; Parotid Gland - ultrastructure ; Phosphocreatine - metabolism ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Archives of physiology and biochemistry, 1996-12, Vol.104 (7), p.826</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9221105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eboué, D</creatorcontrib><creatorcontrib>Sezan, A</creatorcontrib><creatorcontrib>Rossignol, B</creatorcontrib><title>Calcium transport in microsomes isolated from rat parotid gland. Effects of ADP and an ATP-regenerating system</title><title>Archives of physiology and biochemistry</title><addtitle>Arch Physiol Biochem</addtitle><description>Calcium loading of a rat parotid microsomal fraction is greatly increased by an ATP-regenerating system (phosphocreatine and creatine phosphokinase). This effect is neither a consequence of a rise in the ATP concentration nor of an increased formation of inorganic phosphate originating from hydrolysis of ATP or phosphocreatine. Addition of ADP to the incubation medium provokes an inhibition of Ca2+ influx and a stimulation of Ca2+ efflux by the microsomal fraction. These results suggest that the stimulation of Ca2+ uptake by the ATP-regenerating system is due, at least in part, to an increase of Ca2+ influx and a slowing down of Ca2+ efflux as consequence of a decrease of ADP availability. It is proposed that the effect of ADP on Ca2+ movements could account for the action of certain agonists on intracellular Ca2+ concentration. Moreover, the InsP3 responsive Ca2+ pool was also shown to be enlarged by the ATP-regenerating system without modification of InsP3 sensitivity.</description><subject>Adenosine Diphosphate - pharmacology</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Calcium - metabolism</subject><subject>Calcium Radioisotopes - metabolism</subject><subject>Creatine Kinase - metabolism</subject><subject>Inositol 1,4,5-Trisphosphate - metabolism</subject><subject>Ionomycin - pharmacology</subject><subject>Ionophores - pharmacology</subject><subject>Male</subject><subject>Microsomes - metabolism</subject><subject>Parotid Gland - metabolism</subject><subject>Parotid Gland - ultrastructure</subject><subject>Phosphocreatine - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>1381-3455</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFjr0OgjAUhTtoEH8eweS-AIaCJDAS1Dg6uJsKt6SGtuTeMvj2MrA7nJzkO_mSsxKxzEuZ5Oei2Igt8ydNZVZWMhJRlWVSpkUsXKOG1kwWAinHo6cAxoE1LXn2FhkM-0EF7ECTt0AqwKjIB9NBPyjXneCqNbaBwWuoLw-Y2Ryon4-EsEeHs2JcD_zlgHYv1loNjIeld-J4uz6bezJOb4vdayRjFX1fy7_83_4DVQVGJA</recordid><startdate>199612</startdate><enddate>199612</enddate><creator>Eboué, D</creator><creator>Sezan, A</creator><creator>Rossignol, B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199612</creationdate><title>Calcium transport in microsomes isolated from rat parotid gland. Effects of ADP and an ATP-regenerating system</title><author>Eboué, D ; Sezan, A ; Rossignol, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_92211053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>fre</language><creationdate>1996</creationdate><topic>Adenosine Diphosphate - pharmacology</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Calcium - metabolism</topic><topic>Calcium Radioisotopes - metabolism</topic><topic>Creatine Kinase - metabolism</topic><topic>Inositol 1,4,5-Trisphosphate - metabolism</topic><topic>Ionomycin - pharmacology</topic><topic>Ionophores - pharmacology</topic><topic>Male</topic><topic>Microsomes - metabolism</topic><topic>Parotid Gland - metabolism</topic><topic>Parotid Gland - ultrastructure</topic><topic>Phosphocreatine - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eboué, D</creatorcontrib><creatorcontrib>Sezan, A</creatorcontrib><creatorcontrib>Rossignol, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Archives of physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eboué, D</au><au>Sezan, A</au><au>Rossignol, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium transport in microsomes isolated from rat parotid gland. Effects of ADP and an ATP-regenerating system</atitle><jtitle>Archives of physiology and biochemistry</jtitle><addtitle>Arch Physiol Biochem</addtitle><date>1996-12</date><risdate>1996</risdate><volume>104</volume><issue>7</issue><spage>826</spage><pages>826-</pages><issn>1381-3455</issn><abstract>Calcium loading of a rat parotid microsomal fraction is greatly increased by an ATP-regenerating system (phosphocreatine and creatine phosphokinase). This effect is neither a consequence of a rise in the ATP concentration nor of an increased formation of inorganic phosphate originating from hydrolysis of ATP or phosphocreatine. Addition of ADP to the incubation medium provokes an inhibition of Ca2+ influx and a stimulation of Ca2+ efflux by the microsomal fraction. These results suggest that the stimulation of Ca2+ uptake by the ATP-regenerating system is due, at least in part, to an increase of Ca2+ influx and a slowing down of Ca2+ efflux as consequence of a decrease of ADP availability. It is proposed that the effect of ADP on Ca2+ movements could account for the action of certain agonists on intracellular Ca2+ concentration. Moreover, the InsP3 responsive Ca2+ pool was also shown to be enlarged by the ATP-regenerating system without modification of InsP3 sensitivity.</abstract><cop>England</cop><pmid>9221105</pmid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1381-3455 |
| ispartof | Archives of physiology and biochemistry, 1996-12, Vol.104 (7), p.826 |
| issn | 1381-3455 |
| language | fre |
| recordid | cdi_pubmed_primary_9221105 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Adenosine Diphosphate - pharmacology Adenosine Triphosphatases - metabolism Adenosine Triphosphate - metabolism Animals Biological Transport Calcium - metabolism Calcium Radioisotopes - metabolism Creatine Kinase - metabolism Inositol 1,4,5-Trisphosphate - metabolism Ionomycin - pharmacology Ionophores - pharmacology Male Microsomes - metabolism Parotid Gland - metabolism Parotid Gland - ultrastructure Phosphocreatine - metabolism Rats Rats, Sprague-Dawley |
| title | Calcium transport in microsomes isolated from rat parotid gland. Effects of ADP and an ATP-regenerating system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T16%3A22%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Calcium%20transport%20in%20microsomes%20isolated%20from%20rat%20parotid%20gland.%20Effects%20of%20ADP%20and%20an%20ATP-regenerating%20system&rft.jtitle=Archives%20of%20physiology%20and%20biochemistry&rft.au=Ebou%C3%A9,%20D&rft.date=1996-12&rft.volume=104&rft.issue=7&rft.spage=826&rft.pages=826-&rft.issn=1381-3455&rft_id=info:doi/&rft_dat=%3Cpubmed%3E9221105%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/9221105&rfr_iscdi=true |