A Permeabilized Cell System that Assembles Filamentous Bacteriophage
A permeabilized cell system has been developed that is capable of assembling filamentous phage only upon addition of exogenous thioredoxin. The in vitro system exhibits the same component requirements seen in vivo: functional thioredoxin, an intact packaging signal in the substrate DNA, and the asse...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1997-04, Vol.94 (8), p.4068-4073 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Feng, Jian-Nong Russel, Marjorie Model, Peter |
| description | A permeabilized cell system has been developed that is capable of assembling filamentous phage only upon addition of exogenous thioredoxin. The in vitro system exhibits the same component requirements seen in vivo: functional thioredoxin, an intact packaging signal in the substrate DNA, and the assembly protein, pIV. This crude in vitro system is insensitive to inhibitors of protein or DNA synthesis, demonstrating that particle assembly uses components that had accumulated before cell permeabilization. The temporal separation of the synthetic period, during which phage proteins and DNA accumulate, from the assembly period enabled us to examine the energy requirement for assembly. We show here that ATP hydrolysis is required for filamentous phage assembly and that the proton motive force is also important. |
| doi_str_mv | 10.1073/pnas.94.8.4068 |
| format | Article |
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The in vitro system exhibits the same component requirements seen in vivo: functional thioredoxin, an intact packaging signal in the substrate DNA, and the assembly protein, pIV. This crude in vitro system is insensitive to inhibitors of protein or DNA synthesis, demonstrating that particle assembly uses components that had accumulated before cell permeabilization. The temporal separation of the synthetic period, during which phage proteins and DNA accumulate, from the assembly period enabled us to examine the energy requirement for assembly. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1997-04, Vol.94 (8), p.4068-4073 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; Full-Text Journals in Chemistry (Open access); PubMed Central; Alma/SFX Local Collection; JSTOR |
| subjects | Arsenic Bacteriophages Bacteriophages - physiology Biological Sciences Capsid proteins Cell Culture Techniques - methods Cell Membrane - virology Cells Cellular biology Deoxyribonucleic acid DNA DNA Replication DNA, Viral Hydrolysis Nucleotides Packaging Proteins Proton-Motive Force Radiation counters Thioredoxin Virus Replication |
| title | A Permeabilized Cell System that Assembles Filamentous Bacteriophage |
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