Identification of Essential Ionizable Groups in Active Site of Aspergillus niger α-Glucosidase

A kinetic study was done to identify the ionizable groups in the active site of Aspergillus niger α-glucosidase (ANGase). From dependence of V and K m values on pH, we obtained the ionization constants of essential ionizable groups 1 and 2 of free enzyme; pKe 1 = 3.2 and pKe 2 = 6.4. When the dielec...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1997-03, Vol.61 (3), p.475-479
Hauptverfasser:	Kimura, Atsuo, Somoto, Akishige, Mori, Haruhide, Sakai, Osamu, Matsui, Hirokazu, Chiba, Seiya
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Glucosidases - chemistry alpha-Glucosidases - metabolism Aspergillus niger Aspergillus niger - enzymology Bacterial Proteins - chemistry Bacterial Proteins - metabolism Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Carbodiimides - chemistry chemical modification Cysteine - analysis Cysteine - chemistry essential ionizable groups Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hydrogen-Ion Concentration Kinetics Maltose - metabolism Methanol - chemistry Microbiology Mission oriented research Molecular Weight Mycology Physiology and metabolism Substrate Specificity α-glucosidase
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