Expression of neurotrophic factors in cultured human retinal pigment epithelial cells
PURPOSE. To see if cultured human retinal pigment epithelial (RPE) cells have the capacity to synthesize neurotrophins, including nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin-3 (NT-3). METHODS. Expression of mRNAs for the neurotrophins was studied by the reverse tr...
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| Veröffentlicht in: | Current eye research 1997, Vol.16 (2), p.96-101 |
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| creator | Ishida, Kazuhiro Yoshimura, Nagahisa Yoshida, Munenori Honda, Yoshihito Murase, Katsuhito Hayashi, Kyozo |
| description | PURPOSE. To see if cultured human retinal pigment epithelial (RPE) cells have the capacity to synthesize neurotrophins, including nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin-3 (NT-3). METHODS. Expression of mRNAs for the neurotrophins was studied by the reverse transcription polymerase chain reaction (PCR) method. Quantitative analysis of the gene expression was done by using a semiquantitative PCR method. Secretion of NGF-like immunoreactivity (NGF-LI) into the culture medium was analyzed by enzyme immunoassay (EIA). RESULTS. Cultured human RPE cells were found to express mRNAs for NGF, BDNF and NT-3. In the conditioned culture medium of the human RPE, 9.44 ± 0.62 pg/ml (mean ±SEM, n = 6) NGF-LI was found. Pretreatment of human RPE cells with interleukin-1 (IL-1) (20 ng/ml), phorbol myristate acetate (PMA) (100 ng/ml) or tumor necrosis factor-a (TNF-a) (40 ng/ml) was found to increase the mRNA expression of neurotrophins and also to increase secretion of NGF-LI into the culture medium. CONCLUSIONS. Our data demonstrate that cultured human RPE cells have the capacity to synthesize neurotrophins, and that various stimulations can up-regulate gene and protein expression of NGF by these cells. |
| doi_str_mv | 10.1076/ceyr.16.2.96.5093 |
| format | Article |
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To see if cultured human retinal pigment epithelial (RPE) cells have the capacity to synthesize neurotrophins, including nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin-3 (NT-3). METHODS. Expression of mRNAs for the neurotrophins was studied by the reverse transcription polymerase chain reaction (PCR) method. Quantitative analysis of the gene expression was done by using a semiquantitative PCR method. Secretion of NGF-like immunoreactivity (NGF-LI) into the culture medium was analyzed by enzyme immunoassay (EIA). RESULTS. Cultured human RPE cells were found to express mRNAs for NGF, BDNF and NT-3. In the conditioned culture medium of the human RPE, 9.44 ± 0.62 pg/ml (mean ±SEM, n = 6) NGF-LI was found. Pretreatment of human RPE cells with interleukin-1 (IL-1) (20 ng/ml), phorbol myristate acetate (PMA) (100 ng/ml) or tumor necrosis factor-a (TNF-a) (40 ng/ml) was found to increase the mRNA expression of neurotrophins and also to increase secretion of NGF-LI into the culture medium. CONCLUSIONS. Our data demonstrate that cultured human RPE cells have the capacity to synthesize neurotrophins, and that various stimulations can up-regulate gene and protein expression of NGF by these cells.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.1076/ceyr.16.2.96.5093</identifier><identifier>PMID: 9068939</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Brain-Derived Neurotrophic Factor - genetics ; Cells, Cultured ; Fetus ; Gene Expression ; Humans ; Immunoenzyme Techniques ; Nerve Growth Factors - genetics ; Nerve Growth Factors - metabolism ; Neurotrophin 3 ; Pigment Epithelium of Eye - cytology ; Pigment Epithelium of Eye - metabolism</subject><ispartof>Current eye research, 1997, Vol.16 (2), p.96-101</ispartof><rights>1997 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1997</rights><rights>Copyright Oxford University Press Feb 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-835a3192f8008e9129889e06840c1f257382560c97d0c9c5312bfe30436964093</citedby><cites>FETCH-LOGICAL-c494t-835a3192f8008e9129889e06840c1f257382560c97d0c9c5312bfe30436964093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1076/ceyr.16.2.96.5093$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1076/ceyr.16.2.96.5093$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9068939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishida, Kazuhiro</creatorcontrib><creatorcontrib>Yoshimura, Nagahisa</creatorcontrib><creatorcontrib>Yoshida, Munenori</creatorcontrib><creatorcontrib>Honda, Yoshihito</creatorcontrib><creatorcontrib>Murase, Katsuhito</creatorcontrib><creatorcontrib>Hayashi, Kyozo</creatorcontrib><title>Expression of neurotrophic factors in cultured human retinal pigment epithelial cells</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>PURPOSE. To see if cultured human retinal pigment epithelial (RPE) cells have the capacity to synthesize neurotrophins, including nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin-3 (NT-3). METHODS. Expression of mRNAs for the neurotrophins was studied by the reverse transcription polymerase chain reaction (PCR) method. Quantitative analysis of the gene expression was done by using a semiquantitative PCR method. Secretion of NGF-like immunoreactivity (NGF-LI) into the culture medium was analyzed by enzyme immunoassay (EIA). RESULTS. Cultured human RPE cells were found to express mRNAs for NGF, BDNF and NT-3. In the conditioned culture medium of the human RPE, 9.44 ± 0.62 pg/ml (mean ±SEM, n = 6) NGF-LI was found. Pretreatment of human RPE cells with interleukin-1 (IL-1) (20 ng/ml), phorbol myristate acetate (PMA) (100 ng/ml) or tumor necrosis factor-a (TNF-a) (40 ng/ml) was found to increase the mRNA expression of neurotrophins and also to increase secretion of NGF-LI into the culture medium. CONCLUSIONS. Our data demonstrate that cultured human RPE cells have the capacity to synthesize neurotrophins, and that various stimulations can up-regulate gene and protein expression of NGF by these cells.</description><subject>Brain-Derived Neurotrophic Factor - genetics</subject><subject>Cells, Cultured</subject><subject>Fetus</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Nerve Growth Factors - genetics</subject><subject>Nerve Growth Factors - metabolism</subject><subject>Neurotrophin 3</subject><subject>Pigment Epithelium of Eye - cytology</subject><subject>Pigment Epithelium of Eye - metabolism</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UE1rFTEUDaLUZ_UHuBCCi-5mzMdMJkE3UuoHFLpp1yHNu3FSMpMxyaDv3zfDe4gKurkX7j3ncM5B6DUlLSWDeGfhkFoqWtYq0fZE8SdoRztBGsYIe4p2hA204ULy5-hFzg-EbIfuDJ0pIqTiaofurn4uCXL2ccbR4RnWFEuKy-gtdsaWmDL2M7ZrKGuCPR7Xycw4QfGzCXjx3yaYC4bFlxGCrycLIeSX6JkzIcOr0z5Hd5-ubi-_NNc3n79efrxubKe60kjeG04Vc5IQCYoyJaWCaq0jljrWD1yyXhCrhn0dtueU3TvgpONCia7GPUcXR90lxe8r5KInnzcHZoa4Zj1IWcVoV4Fv_wI-xDXVCFlT1TPGOZMVRI8gm2LOCZxekp9MOmhK9Na33vrWVGimldBb35Xz5iS83k-w_8U4FVz_H45_P7uYJvMjprDXxRxCTC6Z2fqs-f_k3_9BH8GEMlqT4LcA_2Q_ArDUpBc</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Ishida, Kazuhiro</creator><creator>Yoshimura, Nagahisa</creator><creator>Yoshida, Munenori</creator><creator>Honda, Yoshihito</creator><creator>Murase, Katsuhito</creator><creator>Hayashi, Kyozo</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Swets & Zeitlinger bv</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Expression of neurotrophic factors in cultured human retinal pigment epithelial cells</title><author>Ishida, Kazuhiro ; Yoshimura, Nagahisa ; Yoshida, Munenori ; Honda, Yoshihito ; Murase, Katsuhito ; Hayashi, Kyozo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-835a3192f8008e9129889e06840c1f257382560c97d0c9c5312bfe30436964093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Brain-Derived Neurotrophic Factor - genetics</topic><topic>Cells, Cultured</topic><topic>Fetus</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Nerve Growth Factors - genetics</topic><topic>Nerve Growth Factors - metabolism</topic><topic>Neurotrophin 3</topic><topic>Pigment Epithelium of Eye - cytology</topic><topic>Pigment Epithelium of Eye - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishida, Kazuhiro</creatorcontrib><creatorcontrib>Yoshimura, Nagahisa</creatorcontrib><creatorcontrib>Yoshida, Munenori</creatorcontrib><creatorcontrib>Honda, Yoshihito</creatorcontrib><creatorcontrib>Murase, Katsuhito</creatorcontrib><creatorcontrib>Hayashi, Kyozo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishida, Kazuhiro</au><au>Yoshimura, Nagahisa</au><au>Yoshida, Munenori</au><au>Honda, Yoshihito</au><au>Murase, Katsuhito</au><au>Hayashi, Kyozo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of neurotrophic factors in cultured human retinal pigment epithelial cells</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>1997</date><risdate>1997</risdate><volume>16</volume><issue>2</issue><spage>96</spage><epage>101</epage><pages>96-101</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><abstract>PURPOSE. To see if cultured human retinal pigment epithelial (RPE) cells have the capacity to synthesize neurotrophins, including nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin-3 (NT-3). METHODS. Expression of mRNAs for the neurotrophins was studied by the reverse transcription polymerase chain reaction (PCR) method. Quantitative analysis of the gene expression was done by using a semiquantitative PCR method. Secretion of NGF-like immunoreactivity (NGF-LI) into the culture medium was analyzed by enzyme immunoassay (EIA). RESULTS. Cultured human RPE cells were found to express mRNAs for NGF, BDNF and NT-3. In the conditioned culture medium of the human RPE, 9.44 ± 0.62 pg/ml (mean ±SEM, n = 6) NGF-LI was found. Pretreatment of human RPE cells with interleukin-1 (IL-1) (20 ng/ml), phorbol myristate acetate (PMA) (100 ng/ml) or tumor necrosis factor-a (TNF-a) (40 ng/ml) was found to increase the mRNA expression of neurotrophins and also to increase secretion of NGF-LI into the culture medium. CONCLUSIONS. Our data demonstrate that cultured human RPE cells have the capacity to synthesize neurotrophins, and that various stimulations can up-regulate gene and protein expression of NGF by these cells.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>9068939</pmid><doi>10.1076/ceyr.16.2.96.5093</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Brain-Derived Neurotrophic Factor - genetics Cells, Cultured Fetus Gene Expression Humans Immunoenzyme Techniques Nerve Growth Factors - genetics Nerve Growth Factors - metabolism Neurotrophin 3 Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - metabolism |
| title | Expression of neurotrophic factors in cultured human retinal pigment epithelial cells |
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