A Synthetic cryIC Gene, Encoding a Bacillus thuringiensis δ -endotoxin, Confers Spodoptera resistance in Alfalfa and Tobacco

Spodoptera species, representing widespread polyphagous insect pests, are resistant to Bacillus thuringiensis δ -endotoxins used thus far as insecticides in transgenic plants. Here we describe the chemical synthesis of a cryIC gene by a novel template directed ligation-PCR method. This simple and ec...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1996-12, Vol.93 (26), p.15012-15017
Hauptverfasser:	Strizhov, Nicolai, Keller, Menachem, Mathur, Jaideep, Koncz-Kalman, Zsuzsanna, Bosch, Dirk, Prudovsky, Evgenia, Schell, Jeff, Sneh, Baruch, Koncz, Csaba, Zilberstein, Aviah
Format:	Artikel
Sprache:	eng
Schlagworte:	Alfalfa Amino Acid Sequence Animals Arabidopsis - physiology Bacillus thuringiensis Toxins Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacterial Toxins Base Sequence Biological Sciences Centrum voor Plantenveredelings- en Reproduktieonderzoek DNA DNA Primers Endotoxins - biosynthesis Endotoxins - genetics Genes, Bacterial Genes, Synthetic Genetic vectors Hemolysin Proteins Insect larvae Leaves Medicago sativa - microbiology Medicago sativa - physiology Molecular Sequence Data Moths Nicotiana - microbiology Nicotiana - physiology Oligonucleotides Pest Control, Biological Plants Plants, Genetically Modified Plants, Toxic Polymerase Chain Reaction Recombinant Proteins - biosynthesis Spodoptera Toxins Transgenic plants
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